The residue level, dissipation behavior, and dietary intake risk of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley (Petroselinum crispum) were investigated under field conditions. Extraction and determination of pesticide residues were carried out by a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and a gas chromatography/tandem mass spectrometry (GC/MS/MS) system, respectively. Dissipation of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley followed the first-order kinetics with a half-life (t1/2) of 3.33, 3.30, 2.94, 3.52, 4.10, and 3.38 days, respectively. Based on the dissipation pattern and the maximum residue limits (MRL), preharvest intervals (PHI) of 25, 13, 18, 24, 1, and 16 days are suggested for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley, respectively. The estimated daily intake (EDI) of pesticides ranged from 7.37E-05 (dimethoate) to 8.00E-04 (metalaxyl) mg/kg. The chronic risk assessment showed that the hazard quotient (HQ) was <1 and Hazard Index (HI, indicating the cumulative exposure to pesticide residues) was <100%, demonstrating that an intake of pesticide residues from parsley was safe for humans.
Key words: half-life, parsley, field conditions, post-harvest interval, GC/MS/MS, method validation
*Corresponding Author: Amin Mousavi Khaneghah, [email protected]
Received: 17-05-2020| Accepted: 09-07-2020| Published: 21-07-2020
© 2020 Codon Publications
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). License (http://creativecommons.org/licenses/by-nc-sa/4.0/)
Parsley (Petroselinum crispum) with bright green color and a mild, bitter flavor is one of the world’s most popular flowering herbs (Charles, 2012; Farzaei et al., 2013). It is mainly cultured as an annual culinary herb in Europe and Western Asia (Charles, 2012). This vegetable is used as a fresh product or a dried spice. It is an excellent source of antioxidants, antibacterial, and antifungal components (Farzaei et al., 2013). The effects of the ingested antioxidant compounds by the consumption of this vegetable on the reduction of cardiovascular disease and some types of cancer have been documented (Brizzolari et al., 2019; Frączek et al., 2019; Fredotović and Puizina, 2019; Shin et al., 2018). Leaves of parsley had various pharmacological attributes, including anticancer, hepatoprotective, neuroprotective, immunosuppressant, anti-diabetic/coagulant so on, and are used to treat gastrointestinal, disorders, kidney stones, inflammation, amenorrhea, and halitosis (Agyare et al., 2017; Charles, 2012; Farzaei et al., 2013).
The use of pesticides is one of the ways to enhance production efficiency (Badawy et al., 2019; Heshmati et al., 2019; Heshmati and Nazemi, 2018). Pesticides are mixtures of chemical compounds for killing, destroying, or mitigating the threat of pests (Pallarés et al., 2020; Serefoglu and Serefoglu, 2016). Although, pesticides prevent or minimize crop loss due to pests, their residues in agricultural products are a major concern with regard to food safety (Aydin and Ulvi, 2019; Hamidi et al., 2019; Nazemi et al., 2016; Shoeibi et al., 2013). In order to maintain human health, pesticide residues in agricultural products and foodstuff should be lower than the maximum residue limit (MRL) set by the legal authority of European Commission (European Commission, 2005; Razzaghi et al., 2018).
As different pests, such as insects and fungi, can grow on parsley (Hershman et al., 1986; Webb, 2006), it is crucial to apply pesticides in order to prevent its yield loss. The occurrence of pesticide residues in parsley has been previously documented (El-Shahawi, 1997; Esturk et al., 2014; Horskáet al., 2020; Szpyrka et al., 2015). Quick, easy, cheap, effective, rugged, and safe sample preparation (QuEChERS) method was one of the most important methods for the extraction of pesticide residues from different food products, including parsley samples (Razzaghi et al., 2018). The advantages of the QuEChERS method are the low cost and fast sample preparation, reduced requirements for reagent, and the simultaneous extraction of several pesticide residues (Kolberg et al., 2011; Lehotay et al., 2010). According to previous studies, gas chromatography (GC), gas chromatography/tandem mass spectrometry (GC/MS/MS), or liquid chromatography/tandem mass spectrometry (LC/MS/MS), are the most commonly used methods or techniques to look for pesticide residues in parsley (Esturk et al., 2014; Horskáet al., 2020). Chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite are commonly used in parsley cultivation in Iran and some other countries (Horskáet al., 2020). While no information (except on metalaxyl) is available regarding persistence and the waiting periods or pre-harvest interval (PHI) of these pesticide residues in parsley. PHI is number of days between the actual application of a pesticide and when a crop can be harvested such that the pesticide residue level has reached below MRL (Horská et al., 2020).Therefore, dissipation studies will help to determine the waiting periods for these pesticide residues in parsley.
In this regard, this study was aimed to determine the dissipation behavior of insecticides (chlorpyrifos-methyl, dimethoate, and permethrin), fungicides (iprodione and metalaxyl) and acaricides (propargite) in parsley under filed conditions, and to assess their risk for the benefit of parsley consumers.
The reference substances of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite were bought from Dr. Ehrenstorfer Co. (Augsburg, Germany). For spraying of parsley, trade forms of mentioned pesticides were purchased from Mahan Inc. (Tehran, Iran). Acetonitrile, sodium chloride, magnesium sulfate all in the analytical grade were purchased from Merck (Darmstadt, Germany). Supelco (Bellefonte, USA) donated the primary secondary amine (PSA), graphitized carbon black (GCB), perfluorotributylamine (PFTBA), and triphenylmethane (TPM). Other chemicals were of analytical grade and were purchased from Merck (Darmstadt, Germany). TMP was utilized as an internal standard. The mass spectrometer performance was checked daily using PFTBA according to the manufacturer’s tuning procedures.
The field experiment was conducted in September of 2018 in Hamadan, Iran. Parsley was cultivated in several separate plots. Each plot had an area of 16 m2 and was sprayed with one type of pesticide using a portable sprayer (Ronix, Model: RH-6005, Tehran, Iran) with standard flat-fan nozzles and a water volume of 12 L. Chlorpyrifos-methyl (Dursban 40.8% water emulsifiable concentrates or EC), dimethoate (Roxion 40% EC), permethrin (Ambush 10% EC), iprodione (52.5% wettable powder or WP), metalaxyl (Ridomil 5 granule or GR), and propargite (Omite 57% water-based emulsion or EW) were separately applied to three test plots at the recommended dosages of 400, 816, 100, 525, 100, and 570 g of active ingredient per hectare (g.a.i./ha), respectively. During the culturing of parsley and the field trial, no rain was reported, and the mean daily temperature, air humidity, and wind speed were 22°C, 32%, and 10 km/h, respectively. Parsley samples were collected at 2 h, and after 2, 4, 6, 10, 15, and 20 days after spraying. During sampling, approximately 1.5 kg of parsley was collected from each plot and transferred to the laboratory. After the addition of dry ice to parsley samples, they were ground with a mill (Romer mill, Stylemaster Drive, USA). Fifty grams of milled samples were weighted and utilized for pesticide residue analysis.
To extract pesticide residue from the parsley sample, the QuEChERS method was applied (Badawy et al., 2020; Heshmati et al., 2019; Hua et al., 2019) according to Figure 1.
Figure 1. The diagram of QuEChERS method used for the extraction of pesticide.
A GC/MS/MS (model 7000C, Agilent, Santa Clara, CA, USA) equipped with an autosampler (model Agilent 7693, Agilent, Santa Clara, CA, USA) and an HP-5 capillary column (30 m × 0.25 mm I.D., 0.25 μm film thicknesses) was used to determine pesticide residue concentration. The oven temperature was programmed as follows: the initial temperature was 75°C, then it was increased to 120°C with a 25°C/min ramp, afterward it was increased to 300°C with a 5°C/min ramp, and this temperature was maintained for 11 min. The helium (purity of 99.99%) was utilized as carrier gas (He) with a flow rate of 1 mL/min. Other conditions of tandem mass spectrometer include the injector temperature, 250°C; the quadrupole temperature, 150°C; and the ion source temperature, 230°C; and ionization energy, 70 eV. The mass spectrometer was operated in the electron ionization (EI) mode and involved multi-reaction monitoring (MRM), while a GC/MS/MS injector was used in the splitless mode. The optimized GC/MS/MS parameters are shown in Table 1.
Table 1. The retention time, diagnostic ions, and the selected quantification ion for the pesticide residue analysis.
Name of pesticide | Retention time | Diagnostic ions | Quantification ion | Collision energy (eV) | Product ion (m/z) | Parent ion (m/z) |
---|---|---|---|---|---|---|
Chlorpyrifos-methyl | 30.04 | 314 (545), 257.8, 197 (999), 199 (956) | 314 | 10 | 168.9 | 196.8 |
Dimethoate | 16.901 | 86.9 (999), 93 (618), 124.8 (502) | 124.8 | 5 | 198.9 | 203 |
Permethrin | 33.930 | 183.0 (999), 164.9 (172), 162.9 (204) | 183.0 | 10 | 149 | 391 |
Iprodion | 29.860 | 186.8 (570), 313.9 (965), 243.7 (150) | 186.8 | 10 | 245 | 313 |
Metalaxyl | 20.710 | 206.1 (999), 159.9 (650), 132.0 (649), 249.0 (499) | 206.1 | 10 | 117 | 132 |
Propargite | 29.113 | 350.2 (20), 201 (150), 134.9 (999), 173 (252) | 350.2 | 15 | 107.1 | 134.8 |
For method validation, linearity, recovery, the limit of detection (LOD), and the limit of quantification (LOQ) were determined. External calibration curves, along with matrix-matched methods, were performed for pesticide residues in the concentration of 0.005–0.400 mg/kg. To assess the accuracy and precision of the analysis method, the recovery experiments were performed. For recovery, blank parsley samples were fortified with 0.005, 0.010, and 0.020 mg/kg of a standard solution of each pesticide. Then, the pesticide residue of spiked samples was extracted and measured following the above procedures. At each fortification level, five replicates were carried out. This experiment was repeated three consecutive days to obtain test precision. Recovery was calculated from the dividing of found pesticide content in spiked samples to real content. LOD and LOQ were considered as signal-to-noise ratio (S/N) of 3:1 and 10:1, respectively (Maznah et al., 2018).
For long-term risk assessment, the estimated daily intake (EDI), HQ (Hazard Quotient), and Hazard Index (HI) were determined according to the following equations (Cámara et al., 2020; Gui et al., 2019; Milinčićet al., 2020; Pallarés et al., 2020; Tandon, 2016; Yang et al., 2020).
supervised trials median residue (STMR) (mg/kg), FI (food intake, kg/day), bw (body weight, kg), and the accepted daily intake (ADI) (mg/kg bw/day) indicated the supervised trials’ median residue (Dong et al., 2017), daily per capita consumption of parsley (58 g) in Iran (Heshmati et al., 2020a; ISIRI, 2010; Mehri et al., 2019), the bodyweight of adults (70 kg), and the ADI, respectively. The STMR is the median of pesticide residues’ level in the edible portion of a food substance applied in supervised trials according to maximum good agricultural practice (GAP) conditions (FAO, 2002). HI was the cumulative exposure to pesticide residues, which is estimated by summing HQ of each pesticide residue. If HI exceeds 100%, the risk is not acceptable (Cámara et al., 2020; Milinčićet al., 2020; Yang et al., 2020).
To determine the degradation kinetics of each pesticide, its residue level versus time was plotted, and the maximum R2 (the squared correlation coefficients) for different curves was determined to obtain the best curve for degradation kinetics. The degradation of whole pesticides followed first-order rate equation, that is, Ct = C0e−kt, where C0 and Ct, indicated the level of the pesticide residues after 2 h of spraying on day t, while K and t are the degradation rate constant and time, respectively (Galietta et al., 2010). The t1/2 (necessary time for the reduction of pesticide residues’ concentration to 50% of its initial level) and PHI were determined with the following equations (Chen et al., 2016):
K and t are the degradation rate constant and time, respectively.
MRL of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley was considered as 0.02, 0.02, 0.05, 0.02, 3, and 0.02 mg/kg, respectively (Commission Regulation, 2017a, 2017b, 2017c, 2018a, 2018b, 2019).
The data of method validation is presented in Table 2. The recovery range of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite was 99.02–102.71, 92.73–98.53, 98.53–114.98, 90.69–98.37, 93.15–98.90, and 94.36–104.06%, respectively.
Table 2. Validation method date, including recovery, relative standard deviations (RSDs), calibration equation, determination coefficient (R2), limit of detection (LOD), and limit of quantification (LOQ).
Parameters | Chlorpyrifos-methyl | Dimethoate | Permethrin | Iprodion | Metalaxyl | Propargite | |||
---|---|---|---|---|---|---|---|---|---|
Recovery (%) and RSD (%) | Spiking level (mg/kg) | 0.005 | Mean (%) | 101.32 | 92.73 | 106.16 | 98.37 | 98.84 | 102.46 |
RSD (%) | 1.89 | 2.54 | 3.15 | 4.2 | 2.51 | 3.4 | |||
0.01 | Mean (%) | 102.71 | 98.53 | 114.98 | 90.69 | 98.9 | 104.06 | ||
RSD (%) | 3.1 | 2.98 | 4.55 | 1.09 | 1.41 | 2.65 | |||
0.02 | Mean (%) | 99.02 | 96.23 | 98.53 | 95.4 | 93.15 | 94.36 | ||
RSD (%) | 5.09 | 2.08 | 3.64 | 4.45 | 1.68 | 2.56 | |||
Calibration curve equation | y = 27270x – 276434 | y = 6012/8x – 22339 | y = 23819x – 512182 | y = 24735x – 150292 | y = 29303x + 24821 | y = 21426x – 469851 | |||
95% Confidence intervals of the calibration curve equation | Slope | 26183 to 28358 | 5547 to 6479 | 21059 to 26579 | 23523 to 25946 | 26659 to 31947 | 19208 to 23644 | ||
Y-intercept | –481319 to -71549 | –110092 to 65413 | –1032187 to 7823 | –378530 to 77946 | –473387 to 523029 | –887780 to –51923 | |||
X-intercept | 2.698 to 17.19 | –11.51 to 17.41 | –0.3570 to 40.41 | –3.262 to 14.82 | –19.08 to 15.24 | 2.609 to 38.91 | |||
R2 | 0.9992 | 0.9969 | 0.9931 | 0.9988 | 0.9958 | 0.9945 | |||
LOD (µg/kg) | 1.78 | 0.12 | 3.82 | 1.11 | 1.35 | 2.44 | |||
LOQ (µg/kg) | 5.3 | 0.37 | 11.47 | 3.33 | 4.05 | 7.33 |
The LOD for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite was 1.78, 0.12, 3.82, 1.11, 1.35, and 2.44 µg/kg, respectively. The LOQ values obtained for pesticides were lower than their MRL, and coefficients of determination (R2) of the calibration curve were >0.999. Therefore, the established method was suitable and satisfactory for analysis.
The dissipation curves of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite under field conditions are presented in Figure 2. Two hours after pesticide application, the initial deposits of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite were 4.748, 0.259, 2.757, 2.098, 3.348 and 0.474 mg/kg, respectively. The data regarding the dissipation behavior of the analyzed pesticide in parsley, such as kinetic equation, half-lives, and R2,are given in Table 3. Half-life values for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite were 3.33, 3.30, 2.94, 3.52, 4.10, and 3.38 days, respectively. The highest PHI (25 days) was related to chlorpyrifos-methyl, while metalaxyl had the lowest PHI (1 day). After 28 days in field conditions, residue levels of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite decreased to 98.59, 98.46, 99.20, 98.33, 95.31, and 98.31%, respectively.
Figure 2. Dissipation of A: chlorpyrifos methyl, B: dimethoate, C: permethrin, D: iprodione, E: metalaxyl, and F: propargite in parsley under field conditions.
Table 3. The initial deposits (mg/kg), regression equation, R2, half-life (t1/2), and preharvest intervals (PHI) of pesticides in parsley.
Chlorpyrifos-methyl | Dimethoate | Permethrin | Iprodione | Metalaxyl | Propargite | |
---|---|---|---|---|---|---|
Initial deposits (mg/kg) | 4.748 | 0.259 | 2.757 | 2.098 | 3.348 | 0.474 |
Regression equation | y = 4.0061e–0.208x | y = 0.3347e–0.21 | y = 3.3444e–0.2367x | y = 2.4335e–0.197x | y = 3.455e–0.1694x | y = 0.5031e–0.205x |
R2 | 0.9675 | 0.97683 | 0.9801 | 0.9763 | 0.968 | 0.9776 |
t1/2 (day) | 3.33 | 3.30 | 2.94 | 3.52 | 4.10 | 3.38 |
PHI (day) | 25 | 13 | 18 | 24 | 1 | 16 |
The dissipation pattern of dimethoate in other crops was partly different from our findings. In papaya and guava, t1/2 of dimethoate was reported as 3.07–5.04 and 2.8–3.3 days; both these findings were similar to our results, while t1/2 of this pesticide in chili fruits (1.94 days) and mango (2 days) was lower than the findings (3.30 days) of the present study (Ahuja et al., 2005; Bhattacherjee and Dikshit, 2016; Khan et al., 2009; Varghese et al., 2011). The half-life of dimethoate in our study (3.30 days) was lower than that reported for chilly (4.7 days) and okra (5.21 days) (Waghulde et al., 2011). The PHI of dimethoate in parsley (132 days) was higher than that in papaya (3–5 days), guava (3 days), and mango (6–7 days), while it was lower than that in chili (13.63 days) and pomegranate (31.5 days at the standard dose of application and 43.0 days at a double dose of application) (Ahuja et al., 2005; Bhattacherjee and Dikshit, 2016; Khan et al., 2009; Utture et al., 2012; Varghese et al., 2011).
The half-life (3.33 days) and PHI (25 days) for chlorpyrifos obtained in this study were longer than those reported for green mustard (t1/2: 1.1–1.5 days and PHI: 4 days) (Chai et al., 2009). In other studies dependent on chlorpyrifos formulates, its t1/2 in orange, peach, tomato, table grape, and wine grape was 8–13, 9.1–14, 6.9–9.1, 7.7–13.9, and 6.6–13.3 days, respectively (Angioni et al., 2011).
The dissipation of metalaxyl has previously been investigated (Chen et al., 2010; Liu et al., 2012; Malhat, 2017; Ramezani and Shahriari, 2015; Rattan and Sharma, 2012). The t1/2 of this pesticide on watermelon, tomato, and cucumber was reported as 3.2–3.5, 1.18, and 3–35 days, respectively. While these findings were lower than that of the current study (4.10 days) (Chen et al., 2010; Malhat, 2017; Rattan and Sharma, 2012); a higher t1/2 (4.9 days) was reported for grape samples (Liu et al., 2012). The calculated waiting period of 1 day for the safe consumption of parsley sprayed with metalaxyl in the current study was similar to corresponding values for cucumber (Ramezani and Shahriari, 2015; Rattan and Sharma, 2012). However, Malhat (2017) reported longer waiting periods (7 days) for tomato (Malhat, 2017). The PHI of pesticides had correlations with their MRL (Heshmati et al., 2020b). A shorter PHI of metalaxyl in parsley was related to greater MRL (3 mg/kg) of this pesticide in comparison with another pesticide.
Propargite is an effective acaricide, which is utilized for a large number of crops (Kumar et al., 2005). The half-life of propargite in green tea leaves, apple, and Brinjal Fruits was, respectively, 1.79, 2.61, 6.2–10m and 3.07–3.54 days (Kang et al., 2009; Kumar et al., 2005; Lou et al., 2008), while t1/2 of this pesticide in our study was 3.38 days. In the current study, we found that a waiting period of 16 days was needed for the safe consumption of parsley to prevent any health hazards due to propargite, while shorter waiting periods were advised for other crops, such as 1 day for the brinjal fruit (Kang et al., 2009).
The half-life of iprodione in our study (3.52 days) was shorter than that in tomato (6.8 days) and similar to that in sweet cherry (3.47 days) (Lazićet al., 2016; Omirou et al., 2009). The occurrence of permethrin was reported for some vegetables, although little published information was found regarding its t1/2 and PHI (George, 1985).
The reasons for discrepancies of t1/2 and PHI values in our study when compared with previous studies were related to the differences in effective factors on pesticide dissipation. The dissipation pattern of pesticides depended on pesticide formulations and physical–chemical properties (vapor pressure and solubility), climate conditions (temperature, humidity, sunlight), plant characteristics (genus and species), location of application of the pesticide, and the number and dosage of pesticide applications (Chai et al., 2009; Heshmati et al., 2020b; Jacobsen et al., 2015; Kumar et al., 2005). Therefore, the variations in these factors in various studies resulted in different t1/2 and PHI values being obtained and reported for each pesticide.
The findings of risk assessment are summarized in Table 4. STMR for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite were 0.876, 0.089, 0.851, 0.750, 0.965, and 0.166 mg/kg, respectively. In a previous study, the estimated STMR of chlorpyrifos in rice and cabbage, respectively, was 0.01 and 0.227 mg/kg (Chen et al., 2012), which was lower than our findings. As shown, the pesticide EDI ranged from 7.37E-05 (dimethoate) to 8.00E-04 (Metalaxyl) mg/kg bw/day, and HQ of all them were <100%. HI of six pesticides was lower than 100%, and this finding is similar to previous studies (Cámara et al., 2020; Milinčićet al., 2020; Yang et al., 2020). Overall, these results showed that for the recommended dose of pesticide, the long-term exposure of consumers to dimethoate, chlorpyrifos-methyl, permethrin, iprodione, metalaxyl, and propargite residues through parsley consumption is relatively negligible. However, it’s necessary to calculate EDI of mentioned pesticide through other crop consumption on which these pesticides may be used.
Table 4. Dietary intake risk assessments of dimethoate, chlorpyrifos methyl, permethrin, iprodione, metalaxyl, and propargite through parsley consumption.
Pesticide | STMR (mg/kg) | EDI (mg/kg bw/day) | ADI (mg/kg bw/day) | HQ (%) |
---|---|---|---|---|
Chlorpyrifos-methyl | 0.876 | 7.26E-04 | 0.010 | 7.26 |
Dimethoate | 0.089 | 7.37E-05 | 0.002 | 3.69 |
Permethrin | 0.851 | 7.05E-04 | 0.050 | 1.41 |
Iprodione | 0.750 | 6.21E-04 | 0.020 | 3.11 |
Metalaxyl | 0.965 | 8.00E-04 | 0.050 | 1.60 |
Propargite | 0.166 | 1.38E-04 | 0.030 | 0.46 |
HI = 17.52 |
HQ: Hazard Quotient; HI, Hazard Index; ADI, accepted daily intake; STMR.
This study is the first report on dissipation behavior and dietary intake risk of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley (Petroselinum crispum) under the field conditions. Dissipation of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley followed the first-order kinetics with t1/2 of 3.33, 3.30, 2.94, 3.52, 4.10, and 3.38, respectively. Based on the dissipation pattern and MRL, PHI of 25, 13, 18, 24, 1, and 16 days are suggested for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley, respectively. The risk assessment showed HQ was <1 for spraying the mentioned pesticide on parsley, and therefore, they were safe for humans. Finally, the results of the current study would be used as a reference for establishing PHI of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley to provide guidance on the safe and proper use of these pesticides. However, the current investigation was devoted to assessing the EDI of pesticides through parsley consumption. It is, however, necessary to calculate EDI of mentioned pesticides through the consumption of other crops.
The authors would like to thank Hamadan University of Medical Science (Project No: 9706203689).
No potential conflict of interest was reported by the authors.
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