Optimisation of ultrasound assisted extraction of rice bran proteins: effects on antioxidant and antiproliferative properties

Main Article Content

E.M. İşçimen
M. Hayta

Keywords

rice bran, ultrasound, optimisation, antioxidant, antiproliferative

Abstract

In this study, it was aimed to increase added value of rice bran which is a by-product of paddy processing. Optimisation of ultrasound assisted extraction produced the highest rice bran protein yield (39.85%) with the parameters as solid/liquid ratio of 0.43, power of 48.25% amplitude, and ultrasound application time of 29.89 min. Based on the optimised solid/liquid ratio and time, antioxidant and antiproliferative properties of protein isolates treated with different level of ultrasound power were determined. Total antioxidant capacity (TEAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity, total phenolic content (TPC), metal chelating activity and the effects on HepG2 cytotoxicity of rice bran protein isolates were evaluated. The highest values of TEAC (17.73%), DPPH (63.61%), TPC (297.43 mg GAE/mg), and metal chelating activity (55.34%) were obtained from samples treated with 100% ultrasound power. Th results of the study showed that the ultrasound power had significant effect (P<0.05) on TPC, metal chelating activity, and DPPH scavenging activity of rice bran proteins.

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