Effect of lactic acid bacterial starter KUB-G2 on grass silage quality and its microbial community performed using 140-ton plastic bag silos: a large-scale study

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Phatthanaphong Therdtatha https://orcid.org/0000-0002-1443-5270
Orawan La-Ongkham
Massalin Nakphaichit
Chaowarit Mapato
Sunthorn Rungruang
Jiro Nakayama
Sunee Nitisinprasert


silage, large-scale silage production, signal grass, lactic acid bacteria, organic acids, microbial community


This study investigated the effectiveness of lactic acid bacteria (LAB) starters in 140 tons of signal grass silage. We compared a locally developed starter, a combination of Lactiplantibacillus plantarum KUB-SP1-3 and Pediococcus acidilactici KUB-M6 (KUB-G2) with a commercial starter. We monitored the chemical and microbiological properties of silage at ensiling (day 0) and after 21 days. The results indicated that KUB-G2 produced high-quality silage. There were no significant differences in total viable microbes, lactic acid, or propionic acid contents between the starters. However, the silage produced with KUB-G2 had a lower acetic acid content than that produced with the commercial starter. KUB-G2 also resulted in a narrower microbiome profile dominated by Pediococci whereas the control group displayed a broader range of bacterial taxa with colonization by Lactiplantibacillus and Lentilactobacillus. These findings suggested that KUB-G2, with its distinct microbial profile, is a suitable replacement for imported commercial starters in large-scale signal grass silage production.

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