SSR markers based DNA fingerprinting and it’s utility in testing purity of eggplant hybrid seeds
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Keywords
field plot test, genetic identity, genetic purity
Abstract
Conventionally, the genetic identity and purity of seeds is assessed by field plot test. However, field plot test method is time consuming, requires large area of land and involves skilled personnel often making subjective decisions. These limitations and the environmental dependency of the entire procedure can be overcome effectively by employing DNA fingerprinting technology. Therefore, the present study was undertaken to identify simple sequence repeat (SSR) markers that could be used to test the genetic identity and purity of six brinjal hybrids and their respective parental lines based on generated DNA fingerprint. In the present study, four markers were found to be polymorphic between parental lines of four respective hybrids (viz. PH-5, PH-9, NDBHL-20 and Kashi Komal) and were found to be suitable for ensuring the genetic identity and purity of these hybrids. Among the identified markers, a set of three markers (emg01B17, emd05F05 and CSM31) could be used for ensuring the identity of the hybrids. Utility of SSR marker based DNA fingerprinting in ensuring the seed purity has been further demonstrated in comparison with that of field plot test.
References
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