Persistence and dissipation behavior of pesticide residues in parsley (Petroselinum crispum) under field conditions

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A Heshmati
H.A. Komacki
F Nazemi
Amin Mousavi Khaneghah


Half-life; parsley; field conditions; post-harvest interval; GC/MS/MS; method validation


The residue level, dissipation behavior, and dietary intake risk of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley (Petroselinum crispum) were investigated under field conditions. Extraction and determination of pesticide residues were carried out by a quick, easy, cheap, effective, rugged, and safe (QuEChERS) method and a gas chromatography/tandem mass spectrometry (GC/MS/MS) system, respec-tively. Dissipation of chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in pars-ley followed the first-order kinetics with a half-life (t1/2) of 3.33, 3.30, 2.94, 3.52, 4.10, and 3.38 days, respectively. Based on the dissipation pattern and the maximum residue limits (MRL), preharvest intervals (PHI) of 25, 13, 18, 24, 1, and 16 days are suggested for chlorpyrifos-methyl, dimethoate, permethrin, iprodione, metalaxyl, and propargite in parsley, respectively. The estimated daily intake (EDI) of pesticides ranged from 7.37E-05 (dimeth-oate) to 8.00E-04 (metalaxyl) mg/kg. The chronic risk assessment showed that the hazard quotient (HQ) was <1 and Hazard Index (HI, indicating the cumulative exposure to pesticide residues) was <100%, demonstrating that an intake of pesticide residues from parsley was safe for humans.

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Agyare, C., Appiah, T., Boakye, Y.D. and Apenteng, J.A., 2017. Chapter 25 – Petroselinum crispum: a review. In: V. Kuete (Ed.), Medicinal Spices and Vegetables from Africa. Academic Press, Cambridge, MA, pp. 527–547. B978-0-12-809286-6.00025-X

Ahuja, A., Mohapatra, S. and Awasthi, M., 2005. Persistence and dissipation of dimethoate and dicofol residues in/on papaya. Pest Management In Horticultural Ecosystems 11: 39–43.

Angioni, A., Dedola, F., Garau, A., Sarais, G., Cabras, P. and Caboni, P., 2011. Chlorpyrifos residues levels in fruits and vegetables after field treatment. Journal of Environmental Science and Health, Part B 46: 544–549.

Aydin, S. and Ulvi, M., 2019. Residue levels of pesticides in nuts and risk assessment for consumers. Quality Assurance and Safety of Crops & Foods 11: 539–548. QAS2018.1405

Badawy, M.E.I., Ismail, A.M.E. and Ibrahim, A.I.H., 2019. Quantitative analysis of acetamiprid and imidacloprid residues in tomato fruits under greenhouse conditions. Journal of Environmental Science and Health, Part B 54: 898–905. http://

Badawy, M.E.I., Mahmoud, M.S. and Khattab, M.M., 2020. Residues and dissipation kinetic of abamectin, chlorfenapyr and pyridaben acaricides in green beans (Phaseolus vulgaris L.) under field conditions using QuEChERS method and HPLC. Journal of Environmental Science and Health, Part B 55:1–8. http://dx.doi. org/10.1080/03601234.2020.1726701

Bhattacherjee, A. and Dikshit, A., 2016. Dissipation kinetics and risk assessment of thiamethoxam and dimethoate in mango. Environmental Monitoring and Assessment 188: 165. http://dx.

Brizzolari, A., Brandolini, A., Glorio-Paulet, P. and Hidalgo, A., 2019. Antioxidant capacity and heat damage of powder products from South American plants with functional properties. Italian Journal of Food Science 31: 731–748.

Cámara, M., Cermeño, S., Martínez, G. and Oliva, J., 2020. Removal residues of pesticides in apricot, peach and orange processed and dietary exposure assessment. Food Chemistry 325:126936.

Chai, L.K., Mohd-Tahir, N. and Bruun Hansen, H.C., 2009. Dissipation of acephate, chlorpyrifos, cypermethrin and their metabolites in a humid-tropical vegetable production system. Pest Management Science 65:189–196. http://dx.doi. org/10.1002/ps.1667

Charles, D., 2012. Parsley. In Handbook of Herbs and Spices. Elsevier, Woodhead Publishing, Sawston, Cambridge, pp. 430–451.

Chen, C., Qian, Y., Liu, X., Tao, C., Liang, Y. and Li, Y., 2012. Risk assessment of chlorpyrifos on rice and cabbage in China. Regulatory Toxicology and Pharmacology 62: 125–130. http://

Chen, L., Jia, C., Zhu, X., He, M., Yu, P. and Zhao, E., 2010. Residual dynamic analysis of metalaxyl-M in watermelon and soil. Agrochemicals 49: 282–283.

Chen, W., Liu, Y. and Jiao, B., 2016. Dissipation behavior of five organophosphorus pesticides in kumquat sample during hon-eyed kumquat candied fruit processing. Food Control 66: 87–92.

Commission Regulation, 2017a. (EU) 2017/623 amending Annexes  II and III to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for acequinocyl, amitraz, coumaphos, diflufenican, flumequine, metribuzin, permethrin, pyraclostrobin and streptomycin in or on certain products. Official Journal of the European Union L 93/1.

Commission Regulation, 2017b. (EU) 2017/1135 amending Annexes  II and III to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for dimethoate and omethoate in or on certain products Official Journal of the European Union L 164/28.

Commission Regulation, 2017c. (EU) 2017/1164 amending Annexes II and III to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for acrinathrin, metalaxyl and thiabendazole in or on certain products. Official Journal of the European Union L 170/3.

Commission Regulation, 2018a. (EU) 2018/686 amending Annexes II and III to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for chlorpyrifos, chlorpyrifos-methyl and triclopyr in or on certain products. Official Journal of the European Union L 121/30.

Commission Regulation, 2018b. (EU) 2018/832 amending Annexes  II, III and V to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for cyantraniliprole, cymoxanil, deltamethrin, difenoconazole, fenamidone, flubendiamide, fluopicolide, folpet, fosetyl, mandestrobin, mepiquat, metazachlor, propamocarb, propargite, pyrimethanil, sulfoxaflor and trifloxystrobin in or on certain products/. Official Journal of the European Union L 140/38.

Commission Regulation, 2019. (EU) 2019/38 amending Annexes II and V to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards maximum residue levels for iprodione in or on certain products. Official Journal of the European Union: L 9/94.

Dong, B., Shao, X., Lin, H. and Hu, J., 2017. Dissipation, residues and risk assessment of metaldehyde and niclosamide ethanol-amine in pakchoi after field application. Food Chemistry 229: 604–609.

El-Shahawi, M., 1997. Retention profiles of some commercial pesticides, pyrethroid and acaricide residues and their application to tomato and parsley plants. Journal of Chromatography A 760: 179–192.

Esturk, O., Yakar, Y. and Ayhan, Z., 2014. Pesticide residue analysis in parsley, lettuce and spinach by LC-MS/MS. Journal of Food Science and Technology 51: 458–466. 10.1007/ s13197-011-0531-9

European Commission, 2005. Regulation (EC) No 396/2005 of the european parliament and of the council of 23 february 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending council directive 91/414/ EEC. Access to the Official Journal 50: 1–50.

FAO (Food and Agriculture Organization), 2002. JMPR Practices in Estimation of Maximum Residue Levels, and Residues Levels for Calculation of Dietary Intake of Pesticide Residues Submission and Evaluation of Pesticide Residues data for the Estimation of Maximum Residue Levels in Food and Feed. FAO. Rome, Italy.

Farzaei, M.H., Abbasabadi, Z., Ardekani, M.R.S., Rahimi, R. and Farzaei, F., 2013. Parsley: a review of ethnopharmacology, phytochemistry and biological activities. Journal of Traditional Chinese Medicine 33: 815–826. S0254-6272(14)60018-2

Fr?czek, B., Morawska, M., Gacek, M. and Pogo?, K., 2019. Antioxidant activity as well as vitamin C and polyphenol con-tent in the diet for athletes. Italian Journal of Food Science 31: 617–630.

Fredotovi?, Ž. and Puizina, J., 2019. Edible allium species: chemical composition, biological activity and health effects. Italian Journal of Food Science 31: 19–39.

Galietta, G., Egaña, E., Gemelli, F., Maeso, D., Casco, N., Conde, P. and Nuñez, S., 2010. Pesticide dissipation curves in peach, pear and tomato crops in Uruguay*. Journal of Environmental Science and Health, Part B 46: 35–40. 03601234.2010.515504

George, D.A., 1985. Permethrin and its two metabolite residues in seven agricultural crops. Journal – Association of Official Analytical Chemists 68: 1160–1163. jaoac/68.6.1160

Gui, T., Jia, G.F., Xu, J., Ge, S.J., Long, X.F., Zhang, Y.P. and Hu, D.Y., 2019. Determination of the residue dynamics and dietary risk of thiamethoxam and its metabolite clothianidin in citrus and soil by LC-MS/MS. Journal of Environmental Science and Health, Part B 54: 326–335. 571361

Hamidi, M., Nili-Ahmadabadi, A. and Heshmati, A., 2019. Evaluation of different preparation methods of edible mush-room (Agaricus bisporus, strains H737) on reduction of health hazards caused by deltamethrin residue. Iranian Journal of Nutrition Sciences & Food Technology 14: 95–104.

Hershman, D., Varney, E. and Johnston, S., 1986. Etiology of parsley damping-off and influence of temperature on disease development. Plant Disease 70: 927–930. PD-70-927

Heshmati, A., Hamidi, M. and Nili-Ahmadabadi, A., 2019. Effect of storage, washing, and cooking on the stability of five pesticides in edible fungi of Agaricus bisporus: a degradation kinetic study. Food Science & Nutrition 7: 3993–4000. http://dx.doi. org/10.1002/fsn3.1261

Heshmati, A., Mehri, F., Karami-Momtaz, J. and Khaneghah, A.M., 2020a. Concentration and risk assessment of potentially toxic elements, lead and cadmium, in vegetables and cereals consumed in Western Iran. Journal of Food Protection 83: 101–107.

Heshmati, A. and Nazemi, F., 2018. Dichlorvos (DDVP) residue removal from tomato by washing with tap and ozone water, a commercial detergent solution and ultrasonic cleaner. Food Science and Technology 38: 441–446.

Heshmati, A., Nili-Ahmadabadi, A., Rahimi, A., Aliasghar, V. and Taheri, M., 2020b. Dissipation behavior and risk assessment of fungicide and insecticide residues in grape under open-field, storage and washing conditions. Journal of Cleaner Production 270:122287.

Horská, T., Kocourek, F., Stará, J., Holý, K., Mráz, P., Krátký, F., Kocourek, V. and Hajšlová, J., 2020. Evaluation of pesticide residue dynamics in lettuce, onion, leek, carrot and parsley. Foods 9: 680.

Hua, J., Fayyaz, A., Song, H., Tufail, M. and Gai, Y., 2019. Development of a method Sin-QuEChERS for the determination of multiple pesticide residues in oilseed samples. Quality Assurance and Safety of Crops & Foods 11: 511–516. http://dx.

ISIRI, 2010. Institute of standards and industrial research of Iran. Food & Feed-Maximum limit of heavy metals. No. 12968. Karaj, Iran.

Jacobsen, R.E., Fantke, P. and Trapp, S., 2015. Analysing half-lives for pesticide dissipation in plants. SAR and QSAR in Environmental Research 26: 325–342. 1062936X.2015.1034772

Kang, B., Jyot, G., Sharma, R., Sahoo, S., Battu, R. and Singh,  B., 2009. Dissipation kinetics of propargite in brinjal fruits under subtropical conditions of Punjab, India. Bulletin of Environmental Contamination and Toxicology 82: 248–250.

Khan, B.A., Farid, A., Asi, M.R., Shah, H. and Badshah, A.K., 2009. Determination of residues of trichlorfon and dimethoate on guava using HPLC. Food Chemistry 114: 286–288. http://dx.doi. org/10.1016/j.foodchem.2008.08.092

Kolberg, D.I., Prestes, O.D., Adaime, M.B. and Zanella, R., 2011. Development of a fast multiresidue method for the determination of pesticides in dry samples (wheat grains, flour and bran) using QuEChERS based method and GC–MS. Food Chemistry 125: 1436–1442. foodchem.2010.10.041

Kumar, V., Sood, C., Jaggi, S., Ravindranath, S., Bhardwaj, S. and Shanker, A., 2005. Dissipation behavior of propargite––an acaricide residues in soil, apple (Malus pumila) and tea (Camellia sinensis). Chemosphere 58: 837–843. chemosphere.2004.06.032

Lazi?, S., Šunjka, D., Pani?, S., Bjelica, Z. and Vukovi?, S., 2016. Dissipation rate and residues of acetamiprid and iprodione in sweet cherry fruits. Agrofor 1:143–150.

Lehotay, S.J., Son, K.A., Kwon, H., Koesukwiwat, U., Fu, W., Mastovska, K., Hoh, E. and Leepipatpiboon, N., 2010. Comparison of QuEChERS sample preparation methods for the analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A 1217: 2548–2560. http://dx.doi. org/10.1016/j.chroma.2010.01.044

Liu, C., Wan, K., Huang, J., Wang, Y. and Wang, F., 2012. Behavior of mixed formulation of metalaxyl and dimethomorph in grape and soil under field conditions. Ecotoxicology and Environmental Safety 84: 112–116. ecoenv.2012.06.030

Lou, Z.-Y., Tang, F.-B., Chen, Z.-M., Luo, F.-J. and Liu, G.-M., 2008. Residue and dissipation dynamics of propargite in citrus and soil [J]. Acta Agriculturae Zhejiangensis 4: 282-286.

Malhat, F.M., 2017. Persistence of metalaxyl residues on tomato fruit using high performance liquid chromatography and QuEChERS methodology. Arabian Journal of Chemistry 10: S765–S768.

Maznah, Z., Halimah, M. and Ismail, B.S., 2018. Evaluation of the persistence and leaching behaviour of thiram fungicide in soil, water and oil palm leaves. Bulletin of Environmental Contamination and Toxicology 100: 677–682. http://dx.doi. org/10.1007/s00128-018-2312-x

Mehri, F., Heshmati, A., Moradi, M. and Khaneghah, A.M., 2019. The concentration and health risk assessment of nitrate in vegetables and fruits samples of Iran. Toxin Reviews 1–8. http://dx.

Milin?i?, D.D., Vojinovi?, U.D., Kosti?, A.Ž., Peši?, M.B., Trifunovi?,  B.D.Š., Brki?, D.V., Stevi?, M.Ž., Koji?, M.O. and Stanisavljevi?, N.S., 2020. In vitro assessment of pesticide residues bioaccessibility in conventionally grown blueberries as affected by complex food matrix. Chemosphere 252:126568.

Nazemi, F., Khodadadi, I. and Heshmati, A., 2016. Effect of storage type and time and washing methods on dichlorvos residues in tomato. Journal of Mazandaran University of Medical Sciences 26: 36–44.

Omirou, M., Vryzas, Z., Papadopoulou-Mourkidou, E. and Economou, A., 2009. Dissipation rates of iprodione and thiacloprid during tomato production in greenhouse. Food Chemistry 116: 499–504.

Pallarés, N., Tolosa, J., Gavahian, M., Barba, F.J., Mousavi-Khaneghah, A. and Ferrer, E., 2020. The potential of pulsed electric fields to reduce pesticides and toxins. In: Pulsed Electric Fields to Obtain Healthier and Sustainable Food for Tomorrow. Elsevier, pp. 141–152.

Ramezani, M.K. and Shahriari, D., 2015. Dissipation behaviour, processing factors and risk assessment for metalaxyl in greenhouse-grown cucumber. Pest Management Science 71: 579–583. http://

Rattan, G. and Sharma, N., 2012. Dissipation kinetics of metalaxyl in cucumber. Bulletin of Environmental Contamination and Toxicology 88: 769–771. s00128-012-0577-z

Razzaghi, N., Ziarati, P., Rastegar, H., Shoeibi, S., Amirahmadi, M., Conti, G.O., Ferrante, M., Fakhri, Y. and Khaneghah, A.M., 2018. The concentration and probabilistic health risk assessment of pesticide residues in commercially available olive oils in Iran. Food and Chemical Toxicology 120: 32–40. http://dx.doi. org/10.1016/j.fct.2018.07.002

Serefoglu, C. and Serefoglu, S., 2016. Consumer fair prices for less pesticide in potato. Italian Journal of Food Science 28: 107–120.

Shin, D., Chae, K., Choi, H., Lee, S., Gim, S., Kwon, G., Lee, H., Song, Y., Kim, K. and Kong, H., 2018. Bioactive and pharmacokinetic characteristics of premature black raspberry, rubus occidentalis. Italian Journal of Food Science 30: 428–439.

Shoeibi, S., Amirahmadi, M., Rastegar, H., Khosrokhavar, R. and Khaneghah, A.M., 2013. An applicable strategy for improvement recovery in simultaneous analysis of 20 pesticides residue in tea. Journal of Food Science 78: T792–T796. http://dx.doi. org/10.1111/1750-3841.12100

Szpyrka, E., Kurdziel, A., Matyaszek, A., Podbielska, M., Rupar, J. and S?owik-Borowiec, M., 2015. Evaluation of pesticide residues in fruits and vegetables from the region of south-eastern Poland. Food Control 48: 137–142. foodcont.2014.05.039

Tandon, S., 2016. Dissipation of pendimethalin in soybean crop under field conditions. Bulletin of Environmental Contamination and Toxicology 96: 694–698. http://dx.doi. org/10.1007/s00128-016-1764-0

Utture, S.C., Banerjee, K., Kolekar, S.S., Dasgupta, S., Oulkar, D.P., Patil, S.H., Wagh, S.S., Adsule, P.G. and Anuse, M.A., 2012. Food safety evaluation of buprofezin, dimethoate and imidacloprid residues in pomegranate. Food Chemistry 131: 787–795. http://

Varghese, T.S., Mathew, T.B., George, T., Beevi, S.N. and Xavier, G., 2011. Dissipation study of dimethoate, ethion and oxydemeton methyl in chilli. Pesticide Research Journal 23: 68–73.

Waghulde, P., Khatik, M., Patil, V. and Patil, P., 2011. Persistence and dissipation of pesticides in chilly and Okra at North Maharashtra Region. Pesticide Research Journal 23: 23–26.

Webb, S., 2006. Insect Management for Celery and Parsley. Department of Entomology and Nematology Document ENY-463, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, Gainesville.

Yang, J., Luo, F., Zhou, L., Sun, H., Yu, H., Wang, X., Zhang, X., Yang,  M., Lou, Z. and Chen, Z., 2020. Residue reduction and risk evaluation of chlorfenapyr residue in tea planting, tea processing, and tea brewing. Science of the Total Environment 738: 139613.