Influence of stress conditions on the quality of obtained sprouts – modification of their chemical composition

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Magdalena Zielińska-Dawidziak


abiotic stress, antioxidants, sprouts nutritional value, sprouts biofortification


Sprouts are generally accepted as a pro-healthy food. They are consumed as a source of valuable macronutrients, antioxidants, microelements, and vitamins. Changing growth conditions of sprouts enables modification of their nutritional quality, as well as their safety. Thus, in order to achieve the most desirable composition of the produced sprouts, the conditions for their production are optimized. The aim of this review is to present methods currently used to modify the nutritional quality of plant sprouts. Most scientific works focus on stress conditions inducing the synthesis of secondary metabolites, mainly antioxidants. An increase in their content is achieved after application of physical (e.g., light illumination, temperature) or chemical factors (e.g., salinity stress, phytohormones, metal ions, etc). Though the application of these modifications on a larger scale is problematic. These problems include difficulties in predicting the effect of the stressor and an increased price of the obtained sprouts. However, since it is possible to enrich sprouts with valuable health-promoting substances, these methods are still considered very promising.

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Abellán Á, Domínguez-Perles R, Moreno D, García-Viguera C. Sorting out the value of cruciferous sprouts as sources of bioactive compounds for nutrition and health. Nutrients. 2019;11(2):429. https//
Andini S, Dekker P, Gruppen H, Araya-Cloutier C, Vincken J-P. Modulation of glucosinolate composition in brassicaceae seeds by germination and fungal elicitation. J Agric Food Chem. 2019;67(46):12770–9. https//
Arscott S, Goldman I. Biomass effects and selenium accumulation in sprouts of three vegetable species grown in selenium-enriched conditions. Hortscience. 2012;47(4):497–502.
Aurisano N, Bertani A, Reggiani R. Anaerobic accumulation of 4-aminobutyrate in rice seedlings; causes and significance. Phytochemistry. 1995;38:1147–50.
Bau H-M, Villaume C, Nicolas J-P, Mejean L. Effect of germination on chemical composition, biochemical constituents and antinutritional factors of soya bean (Glycine max) seeds. J Sci Food Agric. 1997;73:1–9.<1::AID-JSFA694>3.0.CO;2-B
Benincasa P, Falcinelli B, Lutts S, Stagnari F, Galieni A. Sprouted grains: a comprehensive review. Nutrients. 2019 ;11:421.
Boschin G, Resta D. Alkaloids derived from lysine: quinolizidine (a focus on lupin alkaloids). In: Ramawat K, Mérillon JM, editors. Natural products. Berlin, Heidelberg: Springer; 2013. p. 381–403.
Carpici EB, Celik N, Bayram G. Effects of salt stress on germination of some maize (Zea mays L.) cultivars. Afr J Biotechnol. 2009;8(19):4918–22.
Chen Y, Chang SKC. Macronutrients, phytochemicals, and antioxidant activity of soybean sprout germinated with or without light exposure. J Food Sci. 2015;80(6):S1391–S1398.
Commission Implementing Regulation (EU) No 208/2013 of 11 March 2013 on traceability requirements for sprouts and seeds intended for the production of sprouts;
Dell'Aquila A, Spada P. Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress. Seed Sci Res. 1992;2:75–80.
Ding J, Yang T, Feng H, Dong M, Slavin M, Xiong Set al. Enhancing contents of aminobutyric acid (GABA) and other micronutrients in dehulled rice during germination under normoxic and hypoxic conditions. J Agric Food Chem. 2016;64:1094–102.
Domash VI, Sharpio TP, Zabreiko SA, Sosnovskaya TF. Proteolytic enzymes and trypsin inhibitors of higher plants under stress conditions. Russ J Bioorg Chem. 2008;34(3):318–22.
Duenas M, Sarmento T, Aguilera Y, Benitez V, Molla E, Esteban RM, et al. Impact of cooking and germination on phenolic composition and dietary fibre fractions in dark beans (Phaseolus vulgaris L.) and lentils (Lens culinaris L.). LWT - Food Sci Technol. 2016;66:72–8.
Erba D, Angelino D, Marti A, Manini F, Faoro F, Morreale F, et al. Effect of sprouting on nutritional quality of pulses. Int J Food Sci Nutr. 2018;1:11.
Falcinelli B, Benincasa P, Calzuola I, Gigliarelli L, Lutts S, Marsili V. Phenolic content and antioxidant activity in raw and denatured aqueous extracts from sprouts and wheatgrass of einkorn and emmer obtained under salinity. Molecules. 2017;22:2132.
Fan X, Thayer DW, Sokorai KJB. Changes in growth and antioxidant status of alfalfa sprouts during sprouting as affected by gamma irradiation of seeds. J Food Prot. 2004;67(3):561–6.
Gawlik-Dziki U, ?wieca M, Dziki D, Sugier D. Improvement of nutraceutical value of broccoli sprouts by natural elicitors. Acta Scientiarum Polonorum - Hortorum Cultus. 2013;12(1):129–40.
Gull A, Lone AA, Wani NUI. Biotic and abiotic stresses in plants. Abiotic and biotic stress in plants (A.B. de Oliveira), IntechOpen. 2019.
Guo Y, Chen H, Song Y, Gu Z. Effects of soaking and aeration treatment on ?-aminobutyric acid accumulation in germinated soybean (Glycine max L.). Eur Food Res Technol. 2011;232:787–95.
Guo Y, Yang R, Chen H, Song Y, Gu Z. Accumulation of -aminobutyric acid in germinated soybean (Glycine max L.) in relation to glutamate decarboxylase and diamine oxidase activity induced by additives under hypoxia. Eur Food Res Technol. 2012;234:679–87.
Guo Y, Zhu Y, Chen C, Chen X. Effects of aeration treatment on ?-aminobutyric acid accumulation in germinated tartary buckwheat (Fagopyrum tataricum). J Chem. 2016;2016;1–9.
Islam, M.J., Hassan, M.K., Sarker, S.R., Rahman, A.B. & Fakir, M.S.A. 2017. Light and temperature effects on sprout yield and its proximate composition and vitamin C content in lignosus and mung beans. Journal of Bangladesh Agricultural University 15(2): 248-254.
Jamil M, Lee DB, Jung KY, Ashraf M, Lee SC, Rha ES. Effect of salt (NaCl) stress on germination and early seedling growth of four vegetable species. J Cent Eur Agric. 2006;7:273–82.
Kestwal RM, Lin JCh, Bagal-Kestwal D, Chiang BH. Glucosinolates fortification of cruciferous sprouts by sulphur supplementation during cultivation to enhance anti-cancer activity. Food Chem. 2011;126(3):1164–71.
Khattak AB, Zeb A, Bibi N, Khalil SA, Khattak MS.Influence of germination techniques on phytic acid and polyphenols content of chickpea (Cicer arietinum L.) sprouts. Food Chem. 2007;104:1074–9.
Khattak AB, Zeb A, Khan M, Bibi N, Ihsanullah I, Khattak MS. Influence of germination techniques on sprout yield, biosynthesis of ascorbic acid and cooking ability, in chickpea (Cicer arietinum L.). Food Chem. 2006;103(1):115–20. 003
Kim I-D, Dhungana SK, Kim J-K, Ahn H, Kim H-R, Shin D-H. Enhancement of yield and nutritional value of soybean sprouts by persimmon fruit powder. Afr J Biotechnol. 2016;15(44):2490–6.
Koga R, Meng T, Nakamura E, Miura C, Irino N, Devkota HP, et al. The effect of photo-irradiation on the growth and ingredient composition of young green barley (Hordeum vulgare). Agric Sci. 2013;4:185–94.
Kyriacou MC, Rouphael Y, Di Gioia F, Kyratzis A, Serio F, Renna M, et al. Micro-scale vegetable production and the rise of microgreens. Trends Food Sci Technol. 2016;57:103–15.
Le TN, Chiu Ch-H, Hsieh P-Ch. Bioactive compounds and bioactivities of Brassica oleracea L. var. Italica sprouts and microgreens: an updated overview from a nutraceutical perspective. Plants. 2020;9:946.
Lee Y-S, Kim Y-H, Kim S-B. Changes in the respiration, growth, and vitamin C content of soybean sprouts in response to chitosan of different molecular weights. HortScience. 2005;40(5):1333–5.
Li H, Li X, Zhang D, Liu H, Guan K. Effects of drought stress on the seed germination and early seedling growth of the endemic desert plant Eremosparton songoricum (Fabaceae). EXCLI. J. 2013;2:89–101.
Lim JH, Park KJ, Kim BK, Jeong JW, Kim HJ. Effect of salinity stress on phenolic compounds and carotenoids in buckwheat (Fagopyrum esculentum M.) sprout. Food Chem. 2012;135:1065–70.
Liu H, Chen Y, Hu T, Zhang S, Zhang Y, Zhao T, et al. The influence of light-emitting diodes on the phenolic compounds and antioxidant activities in pea sprouts. J Funct Foods. 2016;25:459–65.
Masood T, Shah HU, Zeb A. Effect of sprouting time on proximate composition and ascorbic acid level of mung bean (Vigna radiate L.) and chickpea (Cicer arietinum L.) seeds. J Animal Plant Sci. 2014;24:850–9.
Mastropasqua L, Dipierro N, Paciolla C. Effects of darkness and light spectra on nutrients and pigments in radish, soybean, mung bean and pumpkin sprouts. Antioxidants. 2020;9(6):558.
Mendoza-Sánchez M, Guevara-González RG, Castaño-Tostado E, Mercado-Silva EM, Acosta-Gallegos JA, Rocha-Guzmán NE,et al. Effect of chemical stress on germination of cv Dalia bean (Phaseolus vularis L.) as an alternative to increase antioxidant and nutraceutical compounds in sprouts. Food Chem. 2016;212:128–37.
Meng T, Nakamura E, Irino N, Joshi KR, Devkota HP, Yahara S, et al. Effects of irradiation with light of different photon densities on the growth of young green barley plants. Agric Sci. 2015;6:208–16.
Moreira-Rodríguez M, Nair V, Benavides J, Cisneros-Zevallos L, Jacobo-Velázquez DA. UVA, UVB light, and methyl jasmonate, alone or combined, redirect the biosynthesis of glucosinolates, phenolics, carotenoids, and chlorophylls in broccoli sprouts. Int J Molec Sci. 2017;18:2330.
Nam TG, Kim D-O, Eom SH. Effects of light sources on major flavonoids and antioxidant activity in common buckwheat sprouts. Food Sci Biotechnol. 2017.
Nissar N, Wani SM, Hameed OB, Wani TA, Ahmad M. Influence of paddy (Oryza sativa) sprouting on antioxidant activity, nutritional and anti-nutritional properties. J Food Meas Charact. 2017;11(4):1844–50.
Oh M-M, Rajashekar CB. Antioxidant content of edible sprouts: effects of environmental shocks. J Sci Food Agric. 2009;89(13):2221–7.
Pérez-Balibrea S, Moreno DA, García-Viguera C. Improving the phytochemical composition of broccoli sprouts by elicitation. Food Chem. 2011;129(1):35–44.
Promila K, Kumar S. Vigna radiata seed germination under salinity. Biologia Plantarum. 2000;43(3):423–4.
Przybysz A, Wrochna M, Ma?ecka-Przybysz M, Gawro?ska H, Gawro?ski SW. Vegetable sprouts enriched with iron: effects on yield, ROS generation and antioxidative system. Scientia Horticulturae. 2016;203:110–17.
Przybysz A, Wrochna M, Ma?ecka-Przybysz M, Gawro?ska H, Gawro?ski SW. The effects of Mg enrichment of vegetable sprouts on Mg concentration, yield and ROS generation. J Sci Food Agric. 2015;96(10):3469–76.
Qian H, Liu T, Deng M, Miao H, Cai C, Shen W, et al. Effects of light quality on main health-promoting compounds and antioxidant capacity of Chinese kale sprouts. Food Chem. 2016;196:1232–8.
Qiang L, Qing Z, Xirong Z, Changwei T, Yide H. Application of chitosan and chitosan derivatives into processing of mung bean sprout. J Anhui Agric Univ. 2005;32(3):402–5.
Ramakrishna R, Sarkar D, Shetty K. Metabolic stimulation of phenolic biosynthesis and antioxidant enzyme response in dark germinated barley (Hordeum vulgare L.) sprouts using bioprocessed elicitors. Food Sci Biotechnol. 2019;28:1093–106.
Randhir R, Lin Y-T, Shetty K. Stimulation of phenolics, antioxidant and antimicrobial activities in dark germinated mung bean sprouts in response to peptide and phytochemical elicitors. Process Biochem. 2004a;39:637–46.
Randhir R, Lin Y-T, Shetty K. Phenolics, their antioxidant and antimicrobial activity in dark germinated fenugreek sprouts in response to peptide and phytochemical elicitors. Asia Pacific J Clin Nutr. 2004b;13(3):295–307.
Samuolien? G, Urbonavi?i?t? A, Brazaityt? A, Šabajevien? G, Sakalauskait? J, Duchovskis P. The impact of LED illumination on antioxidant properties of sprouted seeds. Cent Eur J Biol. 2011;6(1):68–74.
Scott, S.J. and Jones, R.A., 1985. Quantifying seed germination responses to low temperatures: variation among Lycopersicon spp. Environmental and Experimental Botany 25: 129–137.
Seo J-M, Arasu MV, Kim Y-B, Park SU, Kim S-J. Phenylalanine and LED lights enhance phenolic compound production in Tartary buckwheat sprouts. Food Chem. 2015;177:204–13.
Shetty P, Atallah MT, Shetty K. Effects of UV treatment on the proline linked pentose phosphate pathway for phenolics and L-DOPA synthesis in dark germinated Vicia faba. Process Biochem. 2002;37:1285–95.
Sim U, Sung J, Lee H, Heo H, Sang Jeong H, Lee J. Effect of calcium chloride and sucrose on the composition of bioactive compounds and antioxidant activities in buckwheat sprouts. Food Chem. 2019;126075.
Stagnari F, Gallieni A, D’Egidio S, Falcinelli B, Pagnani G, Pace R, et al. Effects of sprouting and salt stress on polyphenol composition and antiradical activity of einkorn, emmer and durum wheat. Italian J Agronomy. 2017;12(848):293-301.
Sugihara S, Kondo M, Chihara Y, Yuji M, Hattori H, Yoshida M. Preparation of selenium-enriched sprouts and identification of their selenium species by high-performance liquid chromatography-inductively coupled plasma mass spectrometry. Biosci Biotechnol Biochem. 2004;68(1):193–9.
?wieca M. Production of ready-to-eat lentil sprouts with improved antioxidant capacity: optimization of elicitation conditions with hydrogen peroxide. Food Chem. 2015;180:219-226. 2015;
?wieca M, Baraniak B. Nutritional and antioxidant potential of lentil sprouts affected by elicitation with temperature stress. J Agric Food Chem. 2014;62(14):3306–13.
?wieca M, S?czyk ?, Gawlik-Dziki U. Elicitation and precursor feeding as tools for the improvement of the phenolic content and antioxidant activity of lentil sprouts. Food Chem. 2014;161:288–95.
Tan L, Chen S, Wang T, Dai S. Proteomic insights into seed germination in response to environmental factors. Proteomics. 2013;13(12–13):1850–70.
Tsurunaga T, Takahashi T, Katsube T, Kudo A, Kuramitsu O, Ishiwata M, et al. Effects of UV-B irradiation on the levels of anthocyanin, rutin and radical scavenging activity of buckwheat sprouts. Food Chem. 2013;141(1):552–6.
Tuan PA, Thwe AA, Kim YB, Kim JK, Kim SJ, Lee S, et al. Effects of white, blue, and red light-emitting diodes on carotenoid biosynthetic gene expression levels and carotenoid accumulation in sprouts of tartary buckwheat (Fagopyrum tataricum Gaertn.). J Agric Food Chem. 2013;61:12356–61.
Viacava GE, Roura SI. Principal component and hierarchical cluster analysis to select natural elicitors for enhancing phytochemical content and antioxidant activity of lettuce sprouts. Scientia Horticulturae. 2015;193:13–21.
Wang P, Li X, Tian L, Gu Z, Yang R. Low salinity promotes the growth of broccoli sprouts by regulating hormonal homeostasis and photosynthesis. Hortic Environ Biotechnol. 2019;60:19–30.
Wei J, Miao H, Wang Q. Effect of glucose on glucosinolates, antioxidants and metabolic enzymes in Brassica sprouts. Scientia Horticulturae. 2011;129(4):535–40.
Xu MJ, Dong JF, Zhu MY. Effect of germination conditions on ascorbic acid level and yield of soybean sprout. J Sci Food Agric. 2005;85:943–7.
Yang R, Guo Q, Gu Z. GABA shunt and polyamine degradation pathway on -aminobutyric acid accumulation in germinating fava bean (Vicia faba L.) under hypoxia. Food Chem. 2013;136 152–9.
Ye J, Wen B. Seed germination in relation to the invasiveness in spiny amaranth and edible amaranth in Xishuangbanna, SW China. PLoS ONE. 2017;12(4):e0175948.
Youn YS, Park JK, Jang HD, Rhee YW. Sequential hydration with anaerobic and heat treatment increases GABA (g-aminobutyric acid) content in wheat. Food Chem. 2011;129:1631–5.
Zieli?ska-Dawidziak M. Plant ferritin—a source of iron to prevent its deficiency. Nutrients. 2015;7(2):1184–201.
Zieli?ska-Dawidziak M, Dwiecki K, Lewko K. Modification of soybean and lupine sprouting conditions: influence on yield, ROS generation, and antioxidative systems. Eur Food Res Technol. 2018;244:1945–52.
Zieli?ska-Dawidziak M, Hertig I, Piasecka-Kwiatkowska D, Staniek H, Nowak KW, Twardowski T. Study on iron availability from prepared soybean sprouts using an iron-deficient rat model. Food Chem. 2012;135(4):2622–7.
Zieli?ska-Dawidziak M, Hertig I, Staniek H, Piasecka-Kwiatkowska D, Nowak KW. Effect of iron status in rats on the absorption of metal ions from plant ferritin. Plant Foods Hum Nutr. 2014a;69(2):101–7.
Zieli?ska-Dawidziak M, Piasecka-Kwiatkowska D, Król E, Staniek E, Krejpcio Z. The Safety of Food Supplemented in Iron with Sprouted in Abiotic Stress Legumes Seeds - Heavy Metal Pollution. International Conference on Food Security and Nutrition IPCBEE 2014b;67:23-27.
Zieli?ska-Dawidziak M, Siger A. Effect of elevated accumulation of iron in ferritin on the antioxidants content in soybean sprouts. Eur Food Res Technol. 2012;234:1005–12.
Zieli?ska-Dawidziak M, Staniek H, Król E, Piasecka-Kwiatkowska D, Twardowski T. Legume seeds and cereal grains’ capacity to accumulate iron while sprouting in order to obtain food fortificant. Acta Scientarum Polonorum Technologia Alimentaria. 2016;15(3):333–8.
Zieli?ski H, Koz?owska H. The content of tocopherols in Cruciferae sprouts. Polish J Food Nutr Sci. 2003;12(4):25–31.