Complete mitochondrial genome of Pleurocordyceps sinensis (Hypocreales, Ascomycota), a species with uncertain family-level taxonomic assignment
Main Article Content
Keywords
Cordyceps, hyperparatisism, Polycephalomyces sinensis, taxonomy
Abstract
The complete mitochondrial (mt) genome of the ex-type strain of Pleurocordyceps sinensis, a fungus originally isolated from Ophiocordyceps sinensis, was sequenced, and assembled as a single circular DNA of 31,841 bp. The mt genome encoded 15 conserved proteins (rps3, cox1, cox2, cox3, cob, atp6, atp8, atp9, nad1, nad2, nad3, nad4, nad4L, nad5, and nad6), 2 rRNA (rnl and rns), and 25 tRNA, as well as 10 additional non-conserved open reading frames (ncORFs). Comparative analyses showed that mt genomes within the order Hypocreales encoded the same number and synteny of conserved protein coding genes despite an obvious size variation among this group of fungi. Phylogenetic analyses using 14 conserved protein sequences revealed that this fungus may not belong to the current designated family Ophiocordycipitaceae but is more closely related to the species of Clavicipitaceae. The mt genome presented herein would give valuable information on reconstructing the evolutionary history of clavicipitaceous fungi and also aid in resolving the family-level taxonomic assignment of Polycephalomyces s. l. species.
References
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Kepler, R., Ban, S., Nakagiri, A., Bischoff, J., Hywel-Jones, N., Owensby, C.A. and Spatafora, J.W., 2013. The phylogenetic placement of hypocrealean insect pathogens in the genus Polycephalomyces: an application of one fungus one name. Fungal Biology 117: 611–622. 10.1016/j.funbio.2013.06.002
Kimball, R.T., Guido, M., Hosner, P.A. and Braun, E.L., 2021. When good mitochondria go bad: cyto-nuclear discordance in landfowl (Aves: Galliformes). Gene 801: 145841. 10.1016/j.gene.2021.145841
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Kortsinoglou, A.M., Korovesi, A.G., Theelen, B., Hagen, F., Boekhout, T. and Kouvelis, V.N., 2019. The mitochondrial intergenic regions nad1-cob and cob-rps3 as molecular identification tools for pathogenic members of the genus Cryptococcus. FEMS Yeast Research 19: foz077. 10.1093/femsyr/foz077
Kouvelis, V.N., Ghikas, D.V. and Typas, M.A., 2004. The analysis of the complete mitochondrial genome of Lecanicillium muscarium (synonym Verticillium lecanii) suggests a minimum common gene organization in mtDNAs of Sordariomycetes: phylogenetic implications. Fungal Genetics and Biology 41: 930–940. 10.1016/j.fgb.2004.07.003
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