Role of mould occurrence in aflatoxin build-up and variability of Aspergillus flavus isolates from maize grains across India
Main Article Content
Keywords
aflatoxin B1, ELISA, mycotoxin, RAPD, TLC
Abstract
The present study was conducted to study the role of mould/fungal occurrence on aflatoxin build-up in maize grains and correlation between toxigenic and genetic variability present among isolates of Aspergillus flavus obtained from maize grain samples. Eighty-six maize samples were collected from farmer's fields from various locations among the four maize growing agro-ecological zones of India. Among the microflora of maize grains studied, Aspergillus was the most predominant mould identified. Location Karnal (Haryana) was most sensitive to mould infection; 56.2% of samples), exhibited >20 ?g/kg aflatoxin B1 (AFB1). On the other hand, Begusarai (Bihar) proves to be a less sensitive area for aflatoxin contamination with 90% of samples exhibiting <20 ?g/kg AFB1. Varied isolates of A. flavus from grain samples were established in culture media and studied for toxigenic variability. In vitro high level variability (8,116.61-0.21 ?g/kg) for aflatoxin production potential was found among these isolates. Random amplified polymorphic DNA (RAPD) analysis using 35 OPERON random primers was used to study the correlation between toxigenic and genetic variability. The study exhibited partial relationship between RAPD dendrogram and geographic origin of these isolates, while no correlation was found between genetic variability and toxin production ability among A. flavus isolates studied. Also, three atoxigenic isolates of A. flavus were identified, viz. AF-9, AF-36, and AF-39, endemic to India from the Bihar, Haryana, and Delhi regions, respectively. These strains will need further validation.
References
Abbas, H.K., Zablotowicz, R.M., Weaver, M.A., Horn, B.W., Xie, W. and Shier, W.T., 2004. Comparison of cultural and analytical methods for determination of aflatoxin production by Mississippi Delta Aspergillus isolates. Canadian Journal of Microbiology 50: 193-199.
Bennett, J.W. and Christensen, S.B., 1983. New perspectives on aflatoxin biosynthesis. Advances in Applied Microbiology 29: 53-92.
Cotty, P.J., 2006. Biocompetitive exclusion of toxigenic fungi. In: Barug, D., Bhatnagar, D., Van Egmond, H.P., Van der Kamp, J.W., Van Osenbruggen, W.A. and Visconti, A. (eds.) The mycotoxin factbook, food and feed topics. Wageningen Academic Publishers, Wageningen, the Netherlands, pp. 179-197.
Cotty, P.J., Probst, C. and Jaime-Garcia, R., 2008. Etiologyand management of aflatoxin contamination. In: Leslie, J.F., Bandyopadhyay, R. and Visconti, A. (eds.) Mycotoxins: detection methods, management, public health and agri-cultural trade. CABI, Wallingford, United Kingdom, pp.287-299.
Dhavan, A.S. and Chaudhary, M.R., 1995. Incidence of aflatoxin in animal feedstuffs: a decade scenario in India. Journal of AOAC International 78: 693-698.
Dorner, J.W., 2004. Biological control of aflatoxin contamination of crops. Journal of Toxicology ToxinReviews 23: 435-450.
Gong, Y.Y., Egal, S., Hounsa, A., Turner, P.C., Hall, A.J., Cardwell, K.F. and Wild, P.C., 2003. Determinants of aflatoxin exposure in young children from Benin and Togo, West Africa: the critical role of weaning. International Journal of Epidemiology 32: 556-562.
Hussein, H.S. and Brasel, J.M., 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology 167: 101-134.
International Agency for Research on Cancer (IARC), 1993. Some naturally occurring substances: food items and constituents, heterocyclic aromatic amines and mycotoxins. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 56: 489-521.
Jelinek, C.F., Pohland, A.E. and Wood, G.E., 1989. Worldwide occurrence of mycotoxins in food and feeds-an update. Journal of AOAC International 72: 223-230.
Krishnamachari, K.A.V.R., Bhatt, R.V., Nagarajan, V. and Tilak, T.B.G., 1975. Hepatitis due to Aflatoxicosis-an outbreak in Western India. Lancet 1(7915): 1061-1062.
Kumar,S., Shekhar, M. and Khan, A.A., 2007. A rapid technique for detection of toxigenic and non-toxigenic strains of Aspergillus flavusfrom maize grains. Indian Phytopathology60: 31-34.
Kumar, S., Shekhar, M., Kumari, A., Khan, A.A. and Archana, 2009. Manual for estimation of aflatoxin from maize grains by ELISA and HPLC. DMR Technical Bulletin 1: 22.
Lee, S.H., Kim, J.Y., Kim, S.H., Kim, S.J., Lee, K., Han, S.K., Choi, H.S., Jeong, D.H., An., G. and Kim, S.R., 2004. Trapping and characterization of cold-responsive genes from T-DNA tagging lines in rice. Plant Science 166: 69-79.
Miller, J.D., Young, J.C. and Trenholm, H.L., 1983. Fusarium toxins in field corn. I. Time course of fungal growth and production of deoxynialenol and other mycotoxins. Canadian Journal of Botany 61: 3080-3087.
Nelson, P.E., Tousson, T.A. and Marasas, W.F.O., 1983. Fusarium species: an illustrated manual for identification. Pennsylvania State University Press, State College, PA, USA.
Payne, G.A., 1992. Aflatoxins in maize. Critical Reviews in Plant Sciences 10: 423-440.
Pitt, J.I. and Hocking, A.D., 1997. Food and fungi spoilage. Blackie Academic and Professional Publisher, London, United Kingdom.
Probst, C., Callicott, K.A. and Cotty, P.J., 2012. Deadly strains of Kenyan Aspergillus are distinct from other aflatoxin producers. European Journal of Plant Pathology 132: 419-429.
Shearer, J.F., Sweets, L.E., Baker, N.K. and Tiffany, L.H., 1992. A study of Aspergillus flavus/parasiticus in Iowa crop fields: 1988-1990. Plant Disease 76: 19-22.
Singh, K., Frisvand, J.C., Thrane, U. and Mathur, S.B., 1991. An illustrated manual on identification of some seed-borne aspergilli, fusaria, penicillia and their mycotoxins. Institute of Seed Pathology for Developing Countries, Hellerup, Denmark.
Sinha, K.K., 1990. Incidence of mycotoxin in maize grains in Bihar state, India. Food Additives andContaminants 7: 55-61.
Thimmappa, S., Divakara, A., Aiyaza, M., Hariprasad, P., Nayaka, S.C. and Niranjana, S.R., 2014. Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management. Archives ofPhytopathology and Plant Protection 47: 2141.
Thirmula, D.K., Mayo, M.A., Reddy, G. and Reddy, D.V.R., 2002. Occurrence of aflatoxins and ochratoxin A in Indian poultry feeds. Journal of Food Protection 65: 1338-1340.
Turner, P.C., Moore, S.E., Hall, A.J., Prentice, A.M. and Wild, C.P., 2003. Modification of immune function through exposure to dietary aflatoxin in Gambian children. Environmental Health Perspectives 111: 217-220.
Van Egmond, H.P. and Dekker, W.H., 1995. Worldwide regulations for mycotoxins in 1994. Natural Toxins 3: 332-336.
Vinale, F., Sivasithamparam, K., Ghisalberti, E.L., Wool, S.L., Nigro, Marral, M.R., Lombardi, N., Pascale, A., Ruocco, M., Lanzuise, S., Manganiello, G. and Lorito, M., 2014. Trichoderma secondary metabolites active on plants and fungal pathogens. The Open Mycology Journal 8: 127-139.
Widstrom, N.W., McMillian, W.W., Beaver, R.W. and Wilson, D.M., 1990. Weather-associated changes in aflatoxin contamination of preharvest maize. Journal of Production Agriculture 3: 196-199.
Xianwen, L., Zhang, H., Liu, R. and Liu, C., 2014. Potential for aflatoxin B1 and B2 production by Aspergillus flavus strains isolated from rice samples. Saudi Journal of Biological Sciences 22(2): 176-180.