Comparison of the composition and functional properties of red and purple intermediate wheatgrass (Thinopyrum intermedium) varieties
Main Article Content
Keywords
Perennial wheat, Mineral, Mineral ratio, Phenolics, Antioxidant activity, Anthocyanins
Abstract
Intermediate wheatgrass (IWG; Thinopyrum intermedium) is a promising perennial crop with potential -nutritional and functional benefits. Physical (thousand kernel weight, color), chemical (protein content, mineral composition) and functional (phenolic contents, antioxidant capacity, phenolic acids, anthocyanins, lutein, zeaxanthin, and β-carotene contents) grain characteristics of two IWG varieties, namely, Sova and Filin, were investigated. Protein contents of Sova (red) and Filin (purple) grains were 20.2 and 21.3%, respectively. The Mg, Ca, Mn, Fe, Cu, and Zn contents of Sova were 1575, 1259, 53.3, 51.5, 4.9, and 27.7 mg•kg–1, respectively, and those of Filin were 1560, 1542, 55.7, 59.3, 5.9, and 33.1 mg•kg–1, respectively. Zn:Cu ratios (5.65 for Sova and 5.61 for Filin) were balanced, minimizing risk of Cu deficiency. In both IWG varieties, phenolic contents in the bound fraction and their antioxidant activities (ABTS and CUPRAC) were higher than those in free fraction. Ferulic acid was the most abundant phenolic acid found in the bound fraction of IWG. These findings highlight the nutritional and functional potential of these two IWG varieties, reinforcing their value as promising ingredients for developing health-oriented, sustainable grain-based food products.
References
AACC, International. 2010. The American Association of Cereal Chemists Approved Methods of Analysis 10th ed. The Association, St. Paul, MN., USA.
Abdel-Aal, E.S.M., Hucl, P., and Rabalski, I. 2018. Compositional and antioxidant properties of anthocyanin-rich products prepared from purple wheat. Food Chemistry 254: 13–19. 10.1016/j.foodchem.2018.01.170
Agilent ICP-MS (7850/7800/7900) MassHunter Workstation User Guide. 2022. Agilent Technologies, Inc. USA.
Ajdarov, A.N., Shepelev, S.S., and Shamanin, V.P. 2021. Characteristics of high-stemmed and low-stemmed plants by the components of productivity isolated from the population of large-grain wheatgrass of the gray variety Sova in the conditions of the southern forest-steppe of the Western Siberia. Bulletin of Omsk State Agrarian University 3: 5–16.
ASTM, E 1164. 2002. American Society for Testing and Materials. Standard Practice for Obtaining Spectrophotometric Data for Object-Color Evaluation (No. ASTM E 1164). West Conshohocken, Pennsylvania, USA.
Banjade, J.D., Gajadeera, C., Tyl, C.E., Ismail, B.P., and Schoenfuss, T.C. 2019. Evaluation of dough conditioners and bran refinement on functional properties of intermediate wheatgrass (Thinopyrum intermedium). Journal of Cereal Science. 86: 26–32. 10.1016/j.jcs.2019.01.001
Becker, R., Wagoner, P., Hanners, G.D., and Saunders, R.M. 1991. Compositional, nutritional and functional evaluation of Intermediate Wheatgrass (Thinopyrum Intermedium). Journal of Food Processing and Preservation 15(1): 63–77. 10.1111/j.1745-4549.1991.tb00154.x
Bharathi, R., Muljadi, T., Tyl, C., and Annor, G.A. 2022. Progress on breeding and food processing efforts to improve chemical composition and functionality of intermediate wheatgrass (Thinopyrum intermedium) for the food industry. Cereal Chemistry 99(2): 235–252. 10.1002/cche.10482
Biel, W., Jaroszewska, A., Stankowski, S., Sobolewska, M., and Kępińska-Pacelik, J. 2021. Comparison of yield, chemical composition and farinograph properties of common and ancient wheat grains. European Food Research and Technology. 247(6): 1525–1538. 10.1007/s00217-021-03729-7
Blanch, G.P., and Ruiz del Castillo, M.L. 2021. Effect of baking temperature on the phenolic content and antioxidant activity of black corn (Zea mays L.) bread. Foods 10(6): 1202.
Boakye, P.G., Okyere, A.Y., and Annor, G.A. 2023. Impact of extrusion processing on the nutritional and physicochemical properties of intermediate wheatgrass (Thinopyrum intermedium). Cereal Chemistry 100(3): 628–642. 10.1002/cche.10632
Brett, C.T., and Waldron, K.W., 1996. Physiology and Biochemistry of Plant Cell Walls. Chapman and Hall, London.
Cetiner, B., Koksel, F., Morgounov, A.I., and Koksel, H. 2025. Intermediate wheatgrass (Thinopyrum intermedium): An innovative and sustainable ingredient in cereal-based foods, In: Next-generation Cereal-based Foods and Beverages: Breakthroughs in Technology, Sustainability and Health. Editors: Cristina, M. Rosell and Filiz Koksel. Elsevier
Cetiner, B., Ozdemir, B., Yazar, S., and Koksel, H. 2023a. Comparison of mineral concentration and bioavailability of various modern and old bread wheat varieties grown in Anatolia in around one century. European Food Research. 249(3): 587–596. 10.1007/s00217-022-04153-1
Cetiner, B., Shamanin, V.P., Tekin-Cakmak, Z.H., Pototskaya, I.V., Koksel, F., Shepelev, S.S., Aydarov, A.N., Ozdemir, B., Morgounov, A.I., and Koksel, H. 2023b. Utilization of intermediate wheatgrass (Thinopyrum intermedium) as an innovative ingredient in bread making. Foods 12(11): 2109. 10.3390/foods12112109
Cetiner, B., Tömösközi, S., Török, K., Salantur, A., and Koksel, H. 2020. Comparison of the arabinoxylan composition and physical properties of old and modern bread wheat (Triticum aestivum L.) and landraces genotypes. Cereal Chemistry 97(2): 505–514. 10.1002/cche.10265
Costello, R.B., Rosanoff, A., Dai, Q., Saldanha, L.G., and Potischman, N.A., 2021. Perspective: Characterization of dietary supplements containing calcium and magnesium and their respective ratio—is a rising ratio a cause for concern? Advances in Nutrition 12(2): 291–297. 10.1093/advances/nmaa160
Craine, E.B., and DeHaan, L.R. 2024. Nutritional quality of-early-generation Kernza perennial grain. Agriculture 14(6): 919. 10.3390/agriculture14060919
Crews, T.E., and Cattani, D.J. 2018. Strategies, advances, and-challenges in breeding perennial grain crops. Sustainability 10(7): 2192. 10.3390/su10072192
Cui, L., Ren, Y., Murray, T.D., Yan, W., Guo, Q., Niu, Y., Sun, Y., and Li, H. 2018. Development of perennial wheat through hybridization between wheat and wheatgrasses: A review. Engineering 4(4): 507–513. 10.1016/j.eng.2018.07.003
Dangi, P., Chaudhary, N., Paul, A., Sharma, A., Dutta, I., and Razdan, R. 2023. Pigmented wheat: Nutrition scenario and health benefits. In “Pigmented Cereals and Millets”. S.P. Bangar, S. Maqsood and A.K. Siroha (Ed.), p. 1–26. The Royal Society of Chemistry. 10.1039/9781837670291-00001
DeHaan, L.R., Anderson, J.A., Bajgain, P., Basche, A., Cattani, D.J., Crain, J., Crews, T.E., David, C., Duchene, O., and Gutknecht, J. 2023. Discussion: Prioritize perennial grain development for sustainable food production and environmental benefits. Science of the Total Environment 895: 164975. 10.1016/j.scitotenv.2023.164975
DeLuccia, R., Cheung, M., Ng, T., Ramadoss, R., Altasan, A., and Sukumar, D. 2019. Calcium to magnesium ratio higher than optimal across age groups (P10-100-19). Current Developments in Nutrition 3: Suppl 1. 10.1093/cdn/nzz034.P10-100-19
Del Coco, L., Laddomada, B., Migoni, D., Mita, G., Simeone, R., and Fanizzi, F. 2019. Variability and site dependence of grain mineral contents in tetraploid wheats. Sustainability 11:736. 10.3390/su11030736
DiNicolantonio, J.J., O’Keefe, J.H., and Wilson, W. 2018. Subclinical magnesium deficiency: A principal driver of cardiovascular disease and a public health crisis. Open Heart. 5: e000668.
Durlach, J. 1989. Recommended dietary amounts of magnesium: Mg RDA. Magnesium Research 2:195–203.
Eggersdorfer, M., and Wyss, A. 2018. Carotenoids in human nutrition and health. Archives of Biochemistry and Biophysics 652: 18–26. 10.1016/j.abb.2018.06.001
Ferguson, M., Valsalan, A., Cattani, D., and Malalgoda, M. 2024. Evaluating the quality characteristics of intermediate wheatgrass grown in the Canadian prairies. Cereal Chemistry 101(6): 1246–1260. 10.1002/cche.10823
Fiorentini, D., Cappadone, C., Farruggia, G., and Prata, C. 2021. Magnesium: biochemistry, nutrition, detection, and social impact of diseases linked to its deficiency. Nutrients 13(4):1136. 10.3390/nu13041136
Garg, M., Chawla, M., Chunduri, V., Kumar, R., Sharma, S., Sharma, N.K., Kaur, N., Kumar, A., Mundey, J.K., Saini, M.K., and Singh, S.P. 2016. Transfer of grain colors to elite wheat cultivars and their characterization. Journal of Cereal Science 71: 138–144. 10.1016/j.jcs.2016.08.004
Gazza, L., Galassi, E., Ciccoritti, R., Cacciatori, P., and Pogna, N.E. 2016. Qualitative traits of perennial wheat lines derived from different Thinopyrum species. Genetic Resources and Crop Evolution 63(2): 209–219. 10.1007/s10722-015-0240-8
Geyik, Ö.G., Tekin-Cakmak, Z.H., Shamanin, V.P., Karasu, S., Pototskaya, I.V., Shepelev, S.S., Chursin, A.S., Morgounov, A.I., Yaman, M., Sagdic, O., and Koksel, H. 2023. Effects of phenolic compounds of colored wheats on colorectal cancer cell lines. Quality Assurance and Safety of Crops & Foods. 15(4): 21–31. 10.15586/qas.v15i4.1354
Huang, J.H., Tsai, L.C., Chang, YC., and Cheng, F.C. 2014. High or low calcium intake increases cardiovascular disease risks in older patients with type 2 diabetes. Cardiovascular Diabetology 13(1): 120.
Hussain, A., Larsson, H., Kuktaite, R., and Johansson, E. 2010. Mineral composition of organically grown wheat genotypes: Contribution to daily minerals intake. The International Journal of Environmental Research and Public Health 7(9): 3442–3456. 10.3390/ijerph7093442
ISO, 520. 2010. Cereals and pulses—Determination of the mass of 1000 grains.
Lempereur, I., Rouau, X., and Abecassis, J. 1997. Genetic and agronomic variation in arabinoxylan and ferulic acid contents of Durum wheat (Triticum durum L.) grain and its milling fractions. Journal of Cereal Science 25:103–110. 10.1006/jcrs.1996.0090
Lequart, C., Nuzillard, J.M., Kurek, B., and Debeire, P. 1999. Hydrolysis of wheat bran and straw by an endoxylanase: production and structural characterization of cinnamoyl--oligosaccharides. Carbohydrate Research 319:102–111. 10.1016/S0008-6215(99)00110-X
Li, L., Shewry, P.R., and Ward, J.L. 2008. Phenolic acids in wheat varieties in the HEALTHGRAIN diversity screen. Journal of Agricultural and Food Chemistry 56(21): 9732–9739. 10.1021/jf801069s
Maret, W., and Sandstead, H.H. 2006. Zinc requirements and the risks and benefits of zinc supplementation. Journal of Trace Elements in Medicine and Biology 20(1): 3–18. 10.1016/j.jtemb.2006.01.006
Marti, A., Bock, J.E., Pagani, M.A., Ismail, B., and Seetharaman, K. 2016. Structural characterization of proteins in wheat flour doughs enriched with intermediate wheatgrass (Thinopyrum intermedium) flour. Food Chemistry 194: 994–1002. 10.1016/j.foodchem.2015.08.082
Marti, A., Qiu, X., Schoenfuss, T.C., and Seetharaman, K. 2015. Characteristics of perennial wheatgrass (Thinopyrum intermedium) and refined wheat flour blends: Impact on-rheological properties. Cereal Chemistry 92(5): 434–440. 10.1094/CCHEM-01-15-0017-R
Martínez-Ballesta, M.C., Dominguez-Perles, R., Moreno, D.A., Muries, B., Alcaraz-López, C., Bastías, E., García-Viguera, C., and Carvajal, M. 2010. Minerals in plant food: Effect of agricultural practices and role in human health. A review. Agronomy for Sustainable Development 30(2): 295–309. 10.1051/agro/2009022
Mueller, D., Jung, K., Winter, M., Rogoll, D., Melcher, R., Kulozik, U., Schwarz, K., and Richling, E. 2018. Encapsulation of anthocyanins from bilberries–effects on bioavailability and intestinal accessibility in humans. Food Chemistry 248: 217–224. 10.1016/j.foodchem.2017.12.058
Naczk, M., and Shahidi, F. 2004. Extraction and analysis of-phenolics in food. Journal of Chromatography A 1054(1–2): 95–111. 10.1016/S0021-9673(04)01409-8
Nemli, E., Ozakdogan, S., Tomas, M., McClements, D.J., and Capanoglu, E. 2021. Increasing the bioaccessibility of antioxidants in tomato pomace using excipient emulsions. Food Biophysics 16(3): 355–364. 10.1007/s11483-021-09674-y
Oliveira, W.S., Chen, Q., Edleman, D., Annor, G.A., and Dias, F.F.G. 2024. Unraveling the impacts of germination on the volatile and fatty acid profile of Intermediate Wheatgrass (Thinopyrum intermedium) seeds. Molecules 29(17): 4268. 10.3390/molecules29174268
Ostrowska, A., and Porębska, G. 2017. The content of calcium and magnesium and the Ca:Mg ratio in cultivated plants in the context of human and animal demand for nutrients. Journal of Elementology 22(3): 995–1004. 10.5601/jelem.2016.21.4.1246
Padhy, A.K., Kaur, P., Singh, S., Kashyap, L., and Sharma, A. 2024. Colored wheat and derived products: Key to global nutritional security. Critical Reviews in Food Science and Nutrition 64(7): 1894–1910. 10.1080/10408398.2022.2119366
Parker, M.L., Ng, A., and Waldron, K.W. 2005. The phenolic acid and polysaccharide composition of cell walls of bran layers of mature wheat (Triticum aestivum L. cv. Avalon) grains. Journal of the Science of Food and Agriculture 85(15): 2539–2547. 10.1002/jsfa.2304
Paznocht, L., Kotíková, Z., Orsák, M., Lachman, J., and Martinek, P. 2019. Carotenoid changes of colored-grain wheat flours during bun-making. Food Chemistry 277: 725–734. 10.1016/j.foodchem.2018.11.019
Pimentel, D., Cerasale, D., Stanley, R.C., Perlman, R., Newman, E.M., Brent, L.C., Mullan, A., and Chang, D.T.I. 2012. Annual vs. perennial grain production. Agriculture, Ecosystems & Environment 161: 1–9. 10.1016/j.agee.2012.05.025
Pototskaya, I.V., Shamanin, V.P., Aydarov, A.N., and Morgounov, A.I. 2022. The use of wheatgrass (Thinopyrum intermedium) in breeding. Vavilovskii Zhurnal Genet Selektsii 26(5): 413–421. 10.18699/VJGB-22-51
Quintaes, K.D., and Diez-Garcia, R.W. 2015. The importance of-minerals in the human diet. In “Handbook of Mineral Elements in Food”. M. De La, Guardia and S. Garrigues (Ed.), 1st ed., p. 1–21. Wiley. 10.1002/9781118654316.ch1
Rahardjo, C.P., Gajadeera, C.S., Simsek, S., Annor, G., Schoenfuss, T.C., Marti, A., and Ismail, B.P. 2018. Chemical characterization, functionality, and baking quality of intermediate wheatgrass (Thinopyrum intermedium). Journal of Cereal Science 83: 266–274. 10.1016/j.jcs.2018.09.002
Rietra, R.P.J.J., Heinen, M., Dimkpa C.O., and Bindraban, P.S. 2017. Effects of nutrient antagonism and synergism on yield and fertilizer use efficiency. Communications in Soil Science and Plant Analysis48(16): 1895–1920. 10.1080/00103624.2017.1407429
Rosanoff, A., Dai, Q., and Shapses, S.A. 2016. Essential nutrient interactions: Does low or suboptimal magnesium status interact with vitamin D and/or calcium status? Advances in Nutrition 7(1): 25–43. 10.3945/an.115.008631
Schendel, R.R., Becker, A., Tyl, C.E., and Bunzel, M. 2015. Isolation and characterization of feruloylated arabinoxylan oligosaccharides from the perennial cereal grain intermediate wheat grass (Thinopyrum intermedium). Carbohydrate Research 407: 16–25. 10.1016/j.carres.2015.01.006
Shamanin, V.P., Morgounov, A.I., Aydarov, A.N., Shepelev, S.S., Chursin, A.S., Pototskaya, I.V., Khamova, O.F., and DeHaan, L.R. 2021. Large-grained wheatgrass variety Sova (Thinopyrum intermedium) as an alternative to perennial wheat. Sel’skokhozyaistvennaya Biologiya 56(3): 450–464.
Shamanin, V.P., Tekin-Cakmak, Z.H., Gordeeva, E.I., Karasu, S., Pototskaya, I., Chursin, A.S., Pozherukova, V.E., Ozulku, G., Morgounov, A.I., Sagdic, O., and Koksel, H. 2022. Antioxidant capacity and profiles of phenolic acids in various genotypes of purple wheat. Foods 11(16): 2515. 10.3390/foods11162515
Shamanin, V.P., Tekin-Cakmak, Z.H., Karasu, S., Pototskaya, I.V., Gordeeva, E.I.O., Verner, A., Morgounov, A.I., Yaman, M., Sagdic, O., and Koksel, H. 2024. Antioxidant activity,-anthocyanin profile, and mineral compositions of colored wheats. Quality Assurance and Safety of Crops & Foods 16(1): 98–107. 10.15586/qas.v16i1.1414
Shamanin, V.P., Tekin-Cakmak, Z.H., Karasu, S., Pototskaya, I.V., Shepelev, S.S., Chursin, A.S., Morgounov, A.I., Sagdic, O., and Koksel, H. 2023. Phenolic content and antioxidant capacity of synthetic hexaploid wheats. Plants 12(12): 2301. 10.3390/plants12122301
Sharma, N., Tiwari, V., Vats, S., Kumari, A., Chunduri, V., Kaur, S., Kapoor, P., and Garg, M. 2020. Evaluation of anthocyanin content, antioxidant potential and antimicrobial activity of black, purple and blue colored wheat flour and wheat-grass juice against common human pathogens. Molecules 25(24): 5785. 10.3390/molecules25245785
Tang, F.H.M., Crews, T.E., Brunsell, N.A., and Vico, G. 2024. Perennial intermediate wheatgrass accumulates more soil organic carbon than annual winter wheat—a model assessment. Plant Soil 494(1–2): 509–528. 10.1007/s11104-023-06298-8
Taylor, K., Samaddar, S., Schmidt, R., Lundy, M., and Scow, K. 2023. Soil carbon storage and compositional responses of soil microbial communities under perennial grain IWG vs. annual wheat. Soil Biology and Biochemistry. 184: 109111. 10.1016/j.soilbio.2023.109111
Tyl, C., and Ismail, B.P. 2019. Compositional evaluation of perennial wheatgrass (Thinopyrum intermedium) breeding populations. International Journal of Food Science & Technology 54(3): 660–669. 10.1111/ijfs.13925
Whittaker, P. 1998. Iron and zinc interactions in humans. The American Journal of Clinical Nutrition 68(2): 442S–446S. 10.1093/ajcn/68.2.442S
Zhang, X., Ohm, J.B., Haring, S., DeHaan, L.R., and Anderson, J.A. 2015. Towards the understanding of end-use quality in intermediate wheatgrass (Thinopyrum intermedium): High-molecular-weight glutenin subunits, protein polymerization, and mixing characteristics. Journal of Cereal Science66: 81–88. 10.1016/j.jcs.2015.10.008
Zhao, F.J., Su, Y.H., Dunham, S.J., Rakszegi, M., Bedo, Z., McGrath, S.P., and Shewry, P.R. 2009. Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. Journal of Cereal Science 49(2): 290–295. 10.1016/j.jcs.2008.11.007
Abdel-Aal, E.S.M., Hucl, P., and Rabalski, I. 2018. Compositional and antioxidant properties of anthocyanin-rich products prepared from purple wheat. Food Chemistry 254: 13–19. 10.1016/j.foodchem.2018.01.170
Agilent ICP-MS (7850/7800/7900) MassHunter Workstation User Guide. 2022. Agilent Technologies, Inc. USA.
Ajdarov, A.N., Shepelev, S.S., and Shamanin, V.P. 2021. Characteristics of high-stemmed and low-stemmed plants by the components of productivity isolated from the population of large-grain wheatgrass of the gray variety Sova in the conditions of the southern forest-steppe of the Western Siberia. Bulletin of Omsk State Agrarian University 3: 5–16.
ASTM, E 1164. 2002. American Society for Testing and Materials. Standard Practice for Obtaining Spectrophotometric Data for Object-Color Evaluation (No. ASTM E 1164). West Conshohocken, Pennsylvania, USA.
Banjade, J.D., Gajadeera, C., Tyl, C.E., Ismail, B.P., and Schoenfuss, T.C. 2019. Evaluation of dough conditioners and bran refinement on functional properties of intermediate wheatgrass (Thinopyrum intermedium). Journal of Cereal Science. 86: 26–32. 10.1016/j.jcs.2019.01.001
Becker, R., Wagoner, P., Hanners, G.D., and Saunders, R.M. 1991. Compositional, nutritional and functional evaluation of Intermediate Wheatgrass (Thinopyrum Intermedium). Journal of Food Processing and Preservation 15(1): 63–77. 10.1111/j.1745-4549.1991.tb00154.x
Bharathi, R., Muljadi, T., Tyl, C., and Annor, G.A. 2022. Progress on breeding and food processing efforts to improve chemical composition and functionality of intermediate wheatgrass (Thinopyrum intermedium) for the food industry. Cereal Chemistry 99(2): 235–252. 10.1002/cche.10482
Biel, W., Jaroszewska, A., Stankowski, S., Sobolewska, M., and Kępińska-Pacelik, J. 2021. Comparison of yield, chemical composition and farinograph properties of common and ancient wheat grains. European Food Research and Technology. 247(6): 1525–1538. 10.1007/s00217-021-03729-7
Blanch, G.P., and Ruiz del Castillo, M.L. 2021. Effect of baking temperature on the phenolic content and antioxidant activity of black corn (Zea mays L.) bread. Foods 10(6): 1202.
Boakye, P.G., Okyere, A.Y., and Annor, G.A. 2023. Impact of extrusion processing on the nutritional and physicochemical properties of intermediate wheatgrass (Thinopyrum intermedium). Cereal Chemistry 100(3): 628–642. 10.1002/cche.10632
Brett, C.T., and Waldron, K.W., 1996. Physiology and Biochemistry of Plant Cell Walls. Chapman and Hall, London.
Cetiner, B., Koksel, F., Morgounov, A.I., and Koksel, H. 2025. Intermediate wheatgrass (Thinopyrum intermedium): An innovative and sustainable ingredient in cereal-based foods, In: Next-generation Cereal-based Foods and Beverages: Breakthroughs in Technology, Sustainability and Health. Editors: Cristina, M. Rosell and Filiz Koksel. Elsevier
Cetiner, B., Ozdemir, B., Yazar, S., and Koksel, H. 2023a. Comparison of mineral concentration and bioavailability of various modern and old bread wheat varieties grown in Anatolia in around one century. European Food Research. 249(3): 587–596. 10.1007/s00217-022-04153-1
Cetiner, B., Shamanin, V.P., Tekin-Cakmak, Z.H., Pototskaya, I.V., Koksel, F., Shepelev, S.S., Aydarov, A.N., Ozdemir, B., Morgounov, A.I., and Koksel, H. 2023b. Utilization of intermediate wheatgrass (Thinopyrum intermedium) as an innovative ingredient in bread making. Foods 12(11): 2109. 10.3390/foods12112109
Cetiner, B., Tömösközi, S., Török, K., Salantur, A., and Koksel, H. 2020. Comparison of the arabinoxylan composition and physical properties of old and modern bread wheat (Triticum aestivum L.) and landraces genotypes. Cereal Chemistry 97(2): 505–514. 10.1002/cche.10265
Costello, R.B., Rosanoff, A., Dai, Q., Saldanha, L.G., and Potischman, N.A., 2021. Perspective: Characterization of dietary supplements containing calcium and magnesium and their respective ratio—is a rising ratio a cause for concern? Advances in Nutrition 12(2): 291–297. 10.1093/advances/nmaa160
Craine, E.B., and DeHaan, L.R. 2024. Nutritional quality of-early-generation Kernza perennial grain. Agriculture 14(6): 919. 10.3390/agriculture14060919
Crews, T.E., and Cattani, D.J. 2018. Strategies, advances, and-challenges in breeding perennial grain crops. Sustainability 10(7): 2192. 10.3390/su10072192
Cui, L., Ren, Y., Murray, T.D., Yan, W., Guo, Q., Niu, Y., Sun, Y., and Li, H. 2018. Development of perennial wheat through hybridization between wheat and wheatgrasses: A review. Engineering 4(4): 507–513. 10.1016/j.eng.2018.07.003
Dangi, P., Chaudhary, N., Paul, A., Sharma, A., Dutta, I., and Razdan, R. 2023. Pigmented wheat: Nutrition scenario and health benefits. In “Pigmented Cereals and Millets”. S.P. Bangar, S. Maqsood and A.K. Siroha (Ed.), p. 1–26. The Royal Society of Chemistry. 10.1039/9781837670291-00001
DeHaan, L.R., Anderson, J.A., Bajgain, P., Basche, A., Cattani, D.J., Crain, J., Crews, T.E., David, C., Duchene, O., and Gutknecht, J. 2023. Discussion: Prioritize perennial grain development for sustainable food production and environmental benefits. Science of the Total Environment 895: 164975. 10.1016/j.scitotenv.2023.164975
DeLuccia, R., Cheung, M., Ng, T., Ramadoss, R., Altasan, A., and Sukumar, D. 2019. Calcium to magnesium ratio higher than optimal across age groups (P10-100-19). Current Developments in Nutrition 3: Suppl 1. 10.1093/cdn/nzz034.P10-100-19
Del Coco, L., Laddomada, B., Migoni, D., Mita, G., Simeone, R., and Fanizzi, F. 2019. Variability and site dependence of grain mineral contents in tetraploid wheats. Sustainability 11:736. 10.3390/su11030736
DiNicolantonio, J.J., O’Keefe, J.H., and Wilson, W. 2018. Subclinical magnesium deficiency: A principal driver of cardiovascular disease and a public health crisis. Open Heart. 5: e000668.
Durlach, J. 1989. Recommended dietary amounts of magnesium: Mg RDA. Magnesium Research 2:195–203.
Eggersdorfer, M., and Wyss, A. 2018. Carotenoids in human nutrition and health. Archives of Biochemistry and Biophysics 652: 18–26. 10.1016/j.abb.2018.06.001
Ferguson, M., Valsalan, A., Cattani, D., and Malalgoda, M. 2024. Evaluating the quality characteristics of intermediate wheatgrass grown in the Canadian prairies. Cereal Chemistry 101(6): 1246–1260. 10.1002/cche.10823
Fiorentini, D., Cappadone, C., Farruggia, G., and Prata, C. 2021. Magnesium: biochemistry, nutrition, detection, and social impact of diseases linked to its deficiency. Nutrients 13(4):1136. 10.3390/nu13041136
Garg, M., Chawla, M., Chunduri, V., Kumar, R., Sharma, S., Sharma, N.K., Kaur, N., Kumar, A., Mundey, J.K., Saini, M.K., and Singh, S.P. 2016. Transfer of grain colors to elite wheat cultivars and their characterization. Journal of Cereal Science 71: 138–144. 10.1016/j.jcs.2016.08.004
Gazza, L., Galassi, E., Ciccoritti, R., Cacciatori, P., and Pogna, N.E. 2016. Qualitative traits of perennial wheat lines derived from different Thinopyrum species. Genetic Resources and Crop Evolution 63(2): 209–219. 10.1007/s10722-015-0240-8
Geyik, Ö.G., Tekin-Cakmak, Z.H., Shamanin, V.P., Karasu, S., Pototskaya, I.V., Shepelev, S.S., Chursin, A.S., Morgounov, A.I., Yaman, M., Sagdic, O., and Koksel, H. 2023. Effects of phenolic compounds of colored wheats on colorectal cancer cell lines. Quality Assurance and Safety of Crops & Foods. 15(4): 21–31. 10.15586/qas.v15i4.1354
Huang, J.H., Tsai, L.C., Chang, YC., and Cheng, F.C. 2014. High or low calcium intake increases cardiovascular disease risks in older patients with type 2 diabetes. Cardiovascular Diabetology 13(1): 120.
Hussain, A., Larsson, H., Kuktaite, R., and Johansson, E. 2010. Mineral composition of organically grown wheat genotypes: Contribution to daily minerals intake. The International Journal of Environmental Research and Public Health 7(9): 3442–3456. 10.3390/ijerph7093442
ISO, 520. 2010. Cereals and pulses—Determination of the mass of 1000 grains.
Lempereur, I., Rouau, X., and Abecassis, J. 1997. Genetic and agronomic variation in arabinoxylan and ferulic acid contents of Durum wheat (Triticum durum L.) grain and its milling fractions. Journal of Cereal Science 25:103–110. 10.1006/jcrs.1996.0090
Lequart, C., Nuzillard, J.M., Kurek, B., and Debeire, P. 1999. Hydrolysis of wheat bran and straw by an endoxylanase: production and structural characterization of cinnamoyl--oligosaccharides. Carbohydrate Research 319:102–111. 10.1016/S0008-6215(99)00110-X
Li, L., Shewry, P.R., and Ward, J.L. 2008. Phenolic acids in wheat varieties in the HEALTHGRAIN diversity screen. Journal of Agricultural and Food Chemistry 56(21): 9732–9739. 10.1021/jf801069s
Maret, W., and Sandstead, H.H. 2006. Zinc requirements and the risks and benefits of zinc supplementation. Journal of Trace Elements in Medicine and Biology 20(1): 3–18. 10.1016/j.jtemb.2006.01.006
Marti, A., Bock, J.E., Pagani, M.A., Ismail, B., and Seetharaman, K. 2016. Structural characterization of proteins in wheat flour doughs enriched with intermediate wheatgrass (Thinopyrum intermedium) flour. Food Chemistry 194: 994–1002. 10.1016/j.foodchem.2015.08.082
Marti, A., Qiu, X., Schoenfuss, T.C., and Seetharaman, K. 2015. Characteristics of perennial wheatgrass (Thinopyrum intermedium) and refined wheat flour blends: Impact on-rheological properties. Cereal Chemistry 92(5): 434–440. 10.1094/CCHEM-01-15-0017-R
Martínez-Ballesta, M.C., Dominguez-Perles, R., Moreno, D.A., Muries, B., Alcaraz-López, C., Bastías, E., García-Viguera, C., and Carvajal, M. 2010. Minerals in plant food: Effect of agricultural practices and role in human health. A review. Agronomy for Sustainable Development 30(2): 295–309. 10.1051/agro/2009022
Mueller, D., Jung, K., Winter, M., Rogoll, D., Melcher, R., Kulozik, U., Schwarz, K., and Richling, E. 2018. Encapsulation of anthocyanins from bilberries–effects on bioavailability and intestinal accessibility in humans. Food Chemistry 248: 217–224. 10.1016/j.foodchem.2017.12.058
Naczk, M., and Shahidi, F. 2004. Extraction and analysis of-phenolics in food. Journal of Chromatography A 1054(1–2): 95–111. 10.1016/S0021-9673(04)01409-8
Nemli, E., Ozakdogan, S., Tomas, M., McClements, D.J., and Capanoglu, E. 2021. Increasing the bioaccessibility of antioxidants in tomato pomace using excipient emulsions. Food Biophysics 16(3): 355–364. 10.1007/s11483-021-09674-y
Oliveira, W.S., Chen, Q., Edleman, D., Annor, G.A., and Dias, F.F.G. 2024. Unraveling the impacts of germination on the volatile and fatty acid profile of Intermediate Wheatgrass (Thinopyrum intermedium) seeds. Molecules 29(17): 4268. 10.3390/molecules29174268
Ostrowska, A., and Porębska, G. 2017. The content of calcium and magnesium and the Ca:Mg ratio in cultivated plants in the context of human and animal demand for nutrients. Journal of Elementology 22(3): 995–1004. 10.5601/jelem.2016.21.4.1246
Padhy, A.K., Kaur, P., Singh, S., Kashyap, L., and Sharma, A. 2024. Colored wheat and derived products: Key to global nutritional security. Critical Reviews in Food Science and Nutrition 64(7): 1894–1910. 10.1080/10408398.2022.2119366
Parker, M.L., Ng, A., and Waldron, K.W. 2005. The phenolic acid and polysaccharide composition of cell walls of bran layers of mature wheat (Triticum aestivum L. cv. Avalon) grains. Journal of the Science of Food and Agriculture 85(15): 2539–2547. 10.1002/jsfa.2304
Paznocht, L., Kotíková, Z., Orsák, M., Lachman, J., and Martinek, P. 2019. Carotenoid changes of colored-grain wheat flours during bun-making. Food Chemistry 277: 725–734. 10.1016/j.foodchem.2018.11.019
Pimentel, D., Cerasale, D., Stanley, R.C., Perlman, R., Newman, E.M., Brent, L.C., Mullan, A., and Chang, D.T.I. 2012. Annual vs. perennial grain production. Agriculture, Ecosystems & Environment 161: 1–9. 10.1016/j.agee.2012.05.025
Pototskaya, I.V., Shamanin, V.P., Aydarov, A.N., and Morgounov, A.I. 2022. The use of wheatgrass (Thinopyrum intermedium) in breeding. Vavilovskii Zhurnal Genet Selektsii 26(5): 413–421. 10.18699/VJGB-22-51
Quintaes, K.D., and Diez-Garcia, R.W. 2015. The importance of-minerals in the human diet. In “Handbook of Mineral Elements in Food”. M. De La, Guardia and S. Garrigues (Ed.), 1st ed., p. 1–21. Wiley. 10.1002/9781118654316.ch1
Rahardjo, C.P., Gajadeera, C.S., Simsek, S., Annor, G., Schoenfuss, T.C., Marti, A., and Ismail, B.P. 2018. Chemical characterization, functionality, and baking quality of intermediate wheatgrass (Thinopyrum intermedium). Journal of Cereal Science 83: 266–274. 10.1016/j.jcs.2018.09.002
Rietra, R.P.J.J., Heinen, M., Dimkpa C.O., and Bindraban, P.S. 2017. Effects of nutrient antagonism and synergism on yield and fertilizer use efficiency. Communications in Soil Science and Plant Analysis48(16): 1895–1920. 10.1080/00103624.2017.1407429
Rosanoff, A., Dai, Q., and Shapses, S.A. 2016. Essential nutrient interactions: Does low or suboptimal magnesium status interact with vitamin D and/or calcium status? Advances in Nutrition 7(1): 25–43. 10.3945/an.115.008631
Schendel, R.R., Becker, A., Tyl, C.E., and Bunzel, M. 2015. Isolation and characterization of feruloylated arabinoxylan oligosaccharides from the perennial cereal grain intermediate wheat grass (Thinopyrum intermedium). Carbohydrate Research 407: 16–25. 10.1016/j.carres.2015.01.006
Shamanin, V.P., Morgounov, A.I., Aydarov, A.N., Shepelev, S.S., Chursin, A.S., Pototskaya, I.V., Khamova, O.F., and DeHaan, L.R. 2021. Large-grained wheatgrass variety Sova (Thinopyrum intermedium) as an alternative to perennial wheat. Sel’skokhozyaistvennaya Biologiya 56(3): 450–464.
Shamanin, V.P., Tekin-Cakmak, Z.H., Gordeeva, E.I., Karasu, S., Pototskaya, I., Chursin, A.S., Pozherukova, V.E., Ozulku, G., Morgounov, A.I., Sagdic, O., and Koksel, H. 2022. Antioxidant capacity and profiles of phenolic acids in various genotypes of purple wheat. Foods 11(16): 2515. 10.3390/foods11162515
Shamanin, V.P., Tekin-Cakmak, Z.H., Karasu, S., Pototskaya, I.V., Gordeeva, E.I.O., Verner, A., Morgounov, A.I., Yaman, M., Sagdic, O., and Koksel, H. 2024. Antioxidant activity,-anthocyanin profile, and mineral compositions of colored wheats. Quality Assurance and Safety of Crops & Foods 16(1): 98–107. 10.15586/qas.v16i1.1414
Shamanin, V.P., Tekin-Cakmak, Z.H., Karasu, S., Pototskaya, I.V., Shepelev, S.S., Chursin, A.S., Morgounov, A.I., Sagdic, O., and Koksel, H. 2023. Phenolic content and antioxidant capacity of synthetic hexaploid wheats. Plants 12(12): 2301. 10.3390/plants12122301
Sharma, N., Tiwari, V., Vats, S., Kumari, A., Chunduri, V., Kaur, S., Kapoor, P., and Garg, M. 2020. Evaluation of anthocyanin content, antioxidant potential and antimicrobial activity of black, purple and blue colored wheat flour and wheat-grass juice against common human pathogens. Molecules 25(24): 5785. 10.3390/molecules25245785
Tang, F.H.M., Crews, T.E., Brunsell, N.A., and Vico, G. 2024. Perennial intermediate wheatgrass accumulates more soil organic carbon than annual winter wheat—a model assessment. Plant Soil 494(1–2): 509–528. 10.1007/s11104-023-06298-8
Taylor, K., Samaddar, S., Schmidt, R., Lundy, M., and Scow, K. 2023. Soil carbon storage and compositional responses of soil microbial communities under perennial grain IWG vs. annual wheat. Soil Biology and Biochemistry. 184: 109111. 10.1016/j.soilbio.2023.109111
Tyl, C., and Ismail, B.P. 2019. Compositional evaluation of perennial wheatgrass (Thinopyrum intermedium) breeding populations. International Journal of Food Science & Technology 54(3): 660–669. 10.1111/ijfs.13925
Whittaker, P. 1998. Iron and zinc interactions in humans. The American Journal of Clinical Nutrition 68(2): 442S–446S. 10.1093/ajcn/68.2.442S
Zhang, X., Ohm, J.B., Haring, S., DeHaan, L.R., and Anderson, J.A. 2015. Towards the understanding of end-use quality in intermediate wheatgrass (Thinopyrum intermedium): High-molecular-weight glutenin subunits, protein polymerization, and mixing characteristics. Journal of Cereal Science66: 81–88. 10.1016/j.jcs.2015.10.008
Zhao, F.J., Su, Y.H., Dunham, S.J., Rakszegi, M., Bedo, Z., McGrath, S.P., and Shewry, P.R. 2009. Variation in mineral micronutrient concentrations in grain of wheat lines of diverse origin. Journal of Cereal Science 49(2): 290–295. 10.1016/j.jcs.2008.11.007
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