Acetic acid urea-polyacrylamide gel electrophoresis: a rapid method for testing the genetic purity of sunflower seeds

Main Article Content

J. Zheng
D. Wen
H. Zhao
C. Zhang

Keywords

genetic purity, protein electrophoresis, rapid testing, sunflower seeds

Abstract



Genetic purity testing is an important component of seed quality control. The objective of this study was to develop a rapid method for testing the genetic purity of sunflower seeds. We used six sunflower hybrids and their parental lines to study and improve a method based on the acetic acid urea-polyacrylamide gel electrophoresis (AU-PAGE) approach for testing the genetic purity of maize seeds. The results showed that the most abundant proteins in sunflower seeds were water-soluble proteins, followed by salt-soluble proteins, while there were relatively few other proteins. The AU-PAGE method was improved to increase the clarity of lanes backgrounds by adding an appropriate amount of sodium dodecyl sulfate to the protein extracting solution. The reduced background staining of the lanes improved the bands identification and increased the evidence of protein polymorphism. The method was validated as an efficient method for genetic purity testing of sunflower seeds. The improvements in this method could potentially be applied to other plants and other protein electrophoresis.




 
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