Chemical, physicochemical, pasting and microstructural properties of amaranth (Amaranthus hypochondriacus) flour as affected by different processing treatments

Main Article Content

M. Siwatch
R.B. Yadav
B.S. Yadav


germination, cooking, fermentation, thermal properties, pasting properties


The effect of cooking, germination, and fermentation on the proximate composition, physicochemical, thermal, pasting and microstructural properties of amaranth flour was investigated. The data revealed that protein and amylose content of native and processed amaranth flours ranged from 14.86 to 16.19% and 2.34 to 7.05% respectively. The protein content increased significantly (P?0.05) after germination and fermentation. However, a significant (P?0.05) decrease in amylose content was observed after various processing treatments. Processing treatments (cooking, germination, and fermentation) significantly (P?0.05) reduced the tannin and phytate content and significantly (P?0.05) increased the total phenol content. Also, given processing treatments had varied effect on water absorption capacity, oil absorption capacity, swelling power and solubility, thermal properties and pasting properties. Shape and size of granules were determined by scanning electron microscopy and size of granules ranged from 0.5 to 1?m. The cooked amaranth showed the lowest value whereas fermented amaranth showed the highest value for peak viscosity, hot paste viscosity and cold paste viscosity.

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Adebowale, K.O. and Lawal, O.S., 2003. Functional properties and retrogradation behaviour of native and chemically modified starch of mucuna bean (Mucuna pruriens). Journal of the Science of Food and Agriculture 83: 1541-1546.
Adedeji, O.E., Oyinloye, O.D. and Ocheme, O.B., 2014. Effect of germination time on the functional properties of maize flour and the degree of gelatinization of its cookies. African Journal of Food Science8: 42-47.
Afify, A.M.R., El-Beltagi, H.S., Abd El-Salam, S.M. and Omran, A.A., 2012. Effect of soaking cooking germination and fermentation processing on proximate analysis and mineral content of three white sorghum varieties. Notulae Botanicae Horti Agrobotanici40: 92-98.
Alvarez-Jubete, L., Arendt, E.K. and Gallagher, E., 2010. Nutritive value of pseudocereals and their increasing use as functional glúten-free ingredients. Trends in Food Science and Technology 21: 106-113.
Amadou, I., Gounga, M.E., Shi, Y.H. and Le, G.W., 2014. Fermentation and heat-moisture treatment induced changes on the physicochemical properties of foxtail millet (Setaria italica) flour. Food and Bioproducts Processing 92: 38-45.
Amare, E., Mouquet-Riveir, C., Rochette, I., Adish, A. and Haki, G.D., 2016. Effect of popping and fermentation on proximate composition, minerals and absorption inhibitors and mineral bioavailability of Amaranth caudatus grain cultivated in Ethiopia. Journal of Food Science and Technology 53: 2987-2994.
Association of Official Analytical Chemists (AOAC), 1990. Official methods of analysis, 15th edition. AOAC, Washington, DC, USA.
Baker, L.A. and Rayas-Duarte, P., 1998. Retrogradation of amaranth starch at different storage temperatures and the effects of salt and sugars. Cereal Chemistry 75: 308-314.
Becker, R., Wheeler, E.L., Lorenz, K., Stafford, A.E., Grosjean, O.K., Betschart, A.A. and Saunders, R.M., 1980. A compositional study of amaranth grain. Journal of Food Science 46: 1175-1180.
Bejosano, F.P. and Corke, H., 1998. Effect of Amaranthus and buckwheat proteins on wheat dough properties and noodle quality. Cereal Chemistry 75: 171-176.
Berghofer, E. and Schoenlechner, R., 2002. Grain Amaranth. In: Belton, P. and Taylor, J. (eds.) Pseudocereals and less common cereals, grain properties and utilization potential. Springer-Verlag, Berlin, Germany, pp. 219-260.
Bibi, N., Mehmood, Z., Zeb, A., Khan, M. and Anis, R.A., 2011. Effect of cooking on amylose contents of mungbean (Phaseolus aureus) and chickpea (Cicer arietinum L.) varieties. Journal of Food Science and Engineering 1: 56-61.
Bressani, R., Kalinwosky, L.S., Ortiz, M.A. and Elias, L.G., 1987. Nutritional evaluation of rasted, flaked and popped A. caudatus. Archivos Latinoamericanos de Nutrición 37: 525-531.
Burgos, V.E. and Armada, M., 2015. Characterization and nutritional value of precooked products of kiwicha grains (Amaranthus caudatus). LWT – Food Science and Technology35: 531-538.
Chau, C.F. and Huang Y.L., 2003. Comparison of the chemical composition and physicochemical properties of different fibers prepared from the peel of Citrus sinensis L. cv. Liucheng. Journal of Agriculture and Food Chemistry51: 2615-2618.
Chauhan, A., Saxena, D.C. and Singh, S., 2015. Total dietary fiber and antioxidant activity of gluten free cookies made from raw and germinated amaranth (Amaranthus spp.) flour. LWT – Food Science and Technology 63: 939-945.
Chauhan, A. and Singh, S., 2013. Influence of germination on Physico-chemical properties of amaranth (Amaranthus spp.) flour. International Journal of Agriculture and Food Science and Technology4: 215-220.
Choi, C., Kim, S., Lee, J. and Shin, M., 2004. Properties of amaranth flour processed by various methods. Korean Journal of Food Science and Technology 36: 262-267.
Claver, I.P.H., Zhang, Q.L.I., Kexue, Z. and Zhou, H., 2010. Optimization of ultrasonic extraction of polysaccharides from Chinese malted sorghum using a response surface methodology. Pakistan Journal of Nutrition 9: 336-342.
Colmenares de Ruiz, A.S. and Bressani, R., 1990. Effect of germination on the chemical composition and nutrition value of amaranth grain. Cereal Chemistry 67: 519-522.
Dordevic, T.M., Siler-Marinkovic, S.S. and Dimitrijevic-Brankovic, S.I., 2010. Effect of fermentation on antioxidant properties of some cereals and pseudocereals. Food Chemistry 119: 957-963.
Duenas, M., Hernandez, T., Estrella, I. and Fernandez, I., 2009. Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.). Food Chemistry 117: 599-607.
Dykes, L. and Rooney, L.W., 2006. Sorghum and millet phenols and antioxidants. Journal of Cereal Science 44: 236-251.
El-Khalifa, A.E.O., Schiffler B. and Bernhardt, R., 2005. Effect of fermentation on the functional properties of sorghum flour. Food Chemistry 92: 1-5.
Emambux, M.N. and Taylor, J.N., 2003. Sorghum kafirin interaction with various phenolic compounds. Journal of Science of Food and Agriculture 83: 402-407.
Escudero, N.L., Alabarracín, G.J., López, L. and Giménez, M.S., 2011. Antioxidant activity and phenolic content of flour and protein concentrate of Amaranthus cruentus seeds. Journal of Food Biochemistry 35: 1327-1341.
Fasasi, O.S., 2009. Proximate, antinutritional factors and functional properties of processed pearl millet. Food Science and Technology7: 92-97.
Fletcher, R.J., 2004. Pseudocereals, overview. In: Wrigley, C., Corke, H. and Walker, C.E. (eds.) Encyclopedia of grain science, 1st ed. Elsevier Academic Press, Oxford, UK, pp. 488-493.
Frias, J., Fornal, J., Ring, S.G. and Vidal-Valverde, C., 1998. Effect of germination on Physico-chemical properties of lentil starch and its components. LWT – Food Science and Technology 31: 228-236.
Gamel, T.H., Jozef, P.L., Ahmed, S.M., Ahmed, A.D. and Lila, A.S., 2006. Seed treatments affect functional and anti-nutritional properties of amaranth flours. Journal of the Science of Food Agriculture 86: 1095-1102.
Gutkoski, L.C. and El-Dash, A.A., 1999. Effect of extrusion process variables on physical and chemical properties of extrudate oat products. Plant Foods for Human Nutrition 54: 315-325.
Hassan, A.B., Mohamed Ahmed, I.A., Osman, N.M., Eltayeb, M.M., Osman, G.A. and Babiker, E.E., 2006. Effect of processing treatment followed by fermentation on protein content and digestibility of pearl millet (Pennisetum typhoideum) cultivars. Pakistan Journal of Nutrition5: 86-89.
Haug, W. and Lantzsch, H.J., 1983. Sensitive method for rapid determination of phytate in cereals and cereal products. Journal of Food and Agriculture 34: 1423-1426.
He, D., Han, C., Yao, J., Shen, S. and Yang, P., 2011. Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach. Proteomics 11: 2693-2713.
Hefnawy, T.H., 2011. Effect of processing method on nutritional composition and antinutrient factors in lentils. Annals of Agricultural Sciences 56: 57-61.
Ibrahim, F.S., Babiker, E.E., Nabila, E., Yousif, E. and Abdullahi, H.E.T., 2005. Effect of fermentation on biochemical and sensory characteristics of sorghum flour supplemented with whey protein. Food Chemistry 92: 285-292.
Ilo, S., Liu, Y. and Berghofer, E., 1999. Extrusion cooking of rice flour and amaranth blends. Food Science and Technology 32: 79-88.
Inyang, C.U. and Zakari, U.M., 2008. Effect of germination and fermentation of pearl millet on proximate, chemichal and sensory properties of instant ‘Fura’ – a Nigerian cereal food. Pakistan Journal of Nutrition 7: 9-12.
Jan, R., Saxena, D.C. and Singh, S., 2016. Effect of germination on nutritional, functional, pasting and microstructural properties of Chenopodium flour. Journal of Food Processing and Preservation 41: e12959.
Jood, S., Bishnoi, S. and Sharma, S., 1998. Nutritional and physico-chemical properties of chickpea and lentil cultivars. Nahrung/Food42: 70-73.
Juhasz, R., Gergely, S., Gelencser, T. and Salgo, A., 2005. Relationship between NIR spectra and RVA parameters during wheat germination. Cereal Chemistry 82: 488-493.
Katina, K., Liukkonen, K.H., Kaukovirta-Norja, A., Adlercreutz, H., Heinonen, S.M., Lampi, A.M., Pihlava, J.M. and Poutanen, K., 2007. Fermentation-induced changes in the nutritional value of native or germinated rye. Journal of Cereal Science 46: 348-355.
Khandelwal, S., Udipi, S.A. and Ghugre, P., 2010. Polyphenols and tannins in Indian pulses: effect of soaking, germination and pressure cooking. Food Research International 43: 526-530.
Khattab, R.Y. and Arntfield, S.D., 2009. Nutritional quality of legume seeds as affected by some physical treatments. LWT – Food Science and Technology42: 1113-1118.
Klimczak, I., Malecka, M. and Pacholek, B., 2002. Antioxidant activity of ethanolic extracts of amaranth seeds. Nahrung/Food 3: 184-186.
Kong, X., Bao, J. and Corke, H., 2009. Physical properties of amaranth starch. Food Chemistry113: 371-376.
Kumar, V., Sinha, A.K., Makkar, H.P.S. and Becker, K., 2010. Dietary roles of phytate and phytase in human nutrition: a review. Food Chemistry120: 945-959.
Li, R., Chen, L., Wu, Y., Zhang, R., Baskin, C.C., Baskin, J.M. and Hu, X., 2017. Effects of cultivar and maternal environment on seed quality in Vicia sativa. Frontiers in Plant Science 8: 1411.
Megat Rusydi, M.R. and Azrina, A., 2012. Effect of germination on total phenolic, tannin and phytic acid contents in soy bean and peanut. International Food Research Journal 19: 673-677.
Mendoza, C. and Bressani, R., 1987. Nutritional and functional characteristics of extrusion cooked amaranth flour.Cereal Chemistry 64: 218-222.
Menegassi, B., Leonel, M., Mischan, M.M. and Pinho, S.Z., 2007. Extrusão de farinha de mandioquinha-salsa: efeito da temperatura, rotação e umidade nas características f ísicas dos extrusados. Brazilian Journal of Food Technology 10: 252-258.
Menegassi, B., Pilosof, A.M.R. and Areas, J.A.G., 2011. Comparison of properties of native and extruded amaranth (Amaranthus cruentus L. BRS Alegria) flour. Food Science and Technology 44: 1915-1921.
Mostafa, M.M. and Rahma, E.H., 1987. Chemical and nutritional change in soyabean during germination. Food Chemistry23: 257-275.
Mubarak, A.E., 2005. Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chemistry 89: 489-495.
Muyonga, J.H., Andabati, B. and Ssepuuya, G., 2014. Effect of heat processing on selected grain amaranth physicochemical properties. Food Science and Nutrition 2: 9-16.
Narsih, Yunianta and Harijono, 2012. The study of germination and soaking time to improve nutritional quality of sorghum seed. International Food Research Journal 19: 1429-1432.
Olawoye, B.T. and Gbadamosi, S.O., 2017. Effect of different treatments on in vitro protein digestibility, antinutrients, antioxidant properties and mineral composition of Amaranthus viridis seeds. Cogent Food and Agriculture 3: 1296402.
Oloyede, O.O., James, S., Ocheme, O.B., Chinma, C.E. and Akpa, V.E., 2016. Effects of fermentation time on the functional and pasting properties of defatted Moringa oleifera seed flour. Food Science and Nutrition 4: 89-95.
Onweluzo, J.C. and Nwabugwu, C.C., 2009. Fermentation of millet (Pennisetum americanum) and pegion pea (Cajanus cajan) seeds for flour production: effects on composition and selected functional properties. Pakistan Journal of Nutrition 8: 737-744.
Osman, M.A., 2004. Changes in sorhum enzyme inhibitors, phytic acid, tannins, and in vitro protein digestibility occurring during Khamir (local bread) fermentation. Food Chemistry 88: 129-134.
Perales-Sanchez, J.X., Reyes-Moreno, C., Gomez-Favela, M.A., Milan-Carrillo, J., Cuevas-Rodriguez, E.O., Valdez-Ortiz, A. and Gutierrez-Dorado, R., 2014. Increasing the antioxidant activity, total phenolic and flavonoid contents by optimizing the germination conditions of amaranth seeds. Plant Foods for Human Nutrition 69: 196-202.
Prince, M.L., Van Scoyoc, S. and Butler, L.G., 1978. Critical evaluation of the vanillin reaction as an assay for tannin in sorghum grain. Journal of Agriculture and Food Chemistry 26: 1214-1218.
Sharma, S., Goyal, R. and Barwal, S., 2013. Domestic processing effects on physicochemical, nutritional and anti-nutritional attributes in soybean. International Food Research Journal 20: 3203-3209.
Shimelis, E.A. and Rakshit, S.K., 2007. Effect of processing on antinutrients and in vitro protein digestibility of kidney bean (Phaseolus vulgaris L.) varieties grown in East Africa. Food Chemistry 103: 161-172.
Silva, G.M., Matjaz, T., Manfred, J., Martina, B. and Franc, B., 2009. Nutritional value and use of grain amaranth: potential future application in bread making. Agricultura 6: 43-53.
Simwaka, J.E., Chamba, M.V.M., Huiming, Z., Masamba, K.G. and Luo, Y., 2017. Effect of fermentation on physicochemical and antinutritional factors of complementary foods from millet, sorghum, pumpkin and amaranth seed flours. International Food Research Journal 24: 1869-1879.
Singh, A. Yadav, N. and Sharma, S., 2012. Effect of fermentation on physicochemical properties and in vitro starch and protein digestibility of selected cereals. International Journal of Agriculture and Food Science and Technology2: 66-70.
Sosulski, F.W., Garatt, M.O. and Slinkard, A.E., 1976. Functional properties of ten legume flours. International Journal of Food Science and Technology 9: 66-69.
Uthumporn, U., Nadiah, N.I., Koh, W.Y., Zaibunnisa, A.H. and Azwan, L., 2016. Effect of microwave heating on corn flour and rice flour in water suspensions. International Food Research Journal 23: 2493-2503.
Valdez-Niebla, J.A., Paredes-Lopez, O., Vargas-Lopez, J.M. and Hernandez-Lopez, D., 1993. Moisture sorption isotherms and other physicochemical properties of nixtamilized amaranth flour. Food Chemistry 46: 19-23.
Waliszewski, K.N., Aparicio, M.A., Bello, L.A. and Monray, J.A., 2003. Changes of banana starch by chemical and physical modification. Carbohydrate Polymers52: 237-282.
Williams, P.C., Kuzina, F.D. and Hlyanka, I., 1970. A rapid colorimetric procedure for estimating the amylose content of starches and flours. Cereal Chemistry 47: 411-420.
Xu, J., Zhang, H., Guo, X. and Qian, H., 2012. The impact of germination on the characteristics of brown rice flour and starch. Journal of Science of Food Agriculture 92: 380-387.
Yadav, B.S., Yadav, R.B. and Kumar, M., 2011. Suitability of pigeon pea and rice starches and their blends for noodle making. LWT – Food Science and Technology 44: 1415-1421.
Yadav, R.B., Yadav, B.S. and Dhull, N., 2012. Effect of incorporation of plantain and chickpea flours on the quality characteristics of biscuits. Journal of Food Science and Technology 49: 207-213.
Zang-Hui, L., Li-Te, L., Wei-Hong, M., Wang, F. and Tatsumi, E., 2005. The effects of natural fermentation on the physical properties of rice flour and the rheological characteristics of rice noodles. International Journal of Food Science and Technology 40: 985-992.
Zhou, K. and Yu, L., 2006. Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT – Food Science and Technology39: 1155-1162.
Zhu, L.J., Liu, Q.Q., Sang, Y., Gu, M.H. and Shi, Y.C., 2010. Underlying reasons for waxy rice flours having different pasting properties. Food Chemistry 120: 94-100.