Genetic diversity analysis and DNA fingerprinting of the main japonica rice varieties in Heilongjiang Province

Abstract

In order to apply the national agricultural standard of China (NY/T 1433-2007) and its recommended 12 primers, the simple sequence repeat technique and capillary electrophoresis were firstly applied to analyse the genetic diversity and construct DNA fingerprints of 50 japonica rice cultivars in Heilongjiang Province (including the ChaHaYang, JianSanJiang, WuChang, XiangShui and FangZheng regions). A total of 40 alleles were detected in the 50 varieties using the 12 primer pairs. The number of alleles detected by each primer pair ranged from 1-11, with an average of 3.3. According to the unweighted pair group method with arithmetic mean cluster analysis, the similarity coefficients of the varieties ranged from 0.850-0.982, the polymorphism information content values ranged from 0.093-0.493, and the polymorphic frequencies ranged from 0-0.667. The 50 varieties were divided into eight categories with a similarity coefficient of 3.4. DNA fingerprints were constructed for the 50 rice cultivars. According to the above results, the 12 primers recommended in the national standard were proved to be efficient and applicable for the main japonica rice cultivars in Heilongjiang Province, in which those varieties had narrow genetic backgrounds and low genetic diversity. The genetic background can be expanded by introducing new genetic resources.