Magnetic immobilisation of phospholipase C and its hydrolysis of phospholipids in crude soybean oil

Main Article Content

D. Yu
C. Yu
Y. Jiang
X. Zhang
T. Yuan
L. Wang
W. Elfalleh
L. Jiang


phospholipase C, magnetic immobilisation, stability, oil degumming, 1,2-DAG


In this study, free phospholipase C (PLC) was immobilised onto magnetic carriers, including sodium alginate (MPCSA), magnetic chitosan microparticles (MCM), Fe3O4/P (glycidyl methacrylate (GMA)-EDGM-St) and Fe3O4/SiOx-g-P (GMA). The magnetic immobilised PLC had a broader pH activity and temperature tolerance, which could be separated quickly and reused. The highest enzyme loading and enzymatic activity (135.64 mg/g and 8,560 U/g, respectively) were obtained using the magnetic immobilised PLC-Fe3O4/SiOx-g-P (GMA) system. Its optimum pH range was 5.5-7.0, and activity remained stable between 50 and 70 °C. After 6 cycles in soybean lecithin emulsion, the magnetic immobilised enzyme retained more than 80% of its initial activity. Enzymatic degumming with PLC-Fe3O4/SiOx-g-P (GMA) was carried out 2.0 h at 60 °C, resulting in 1,2-diacylglycerol (1,2-DAG) content of 1.07%, which improving the refining rate of soybean oil. Moreover, PLC-Fe3O4/SiOx-g-P (GMA) still possessed more than 80% of its initial activity after 5 cycles of degumming in crude soybean oil. The results show that magnetic immobilised PLC has a good industrial application.

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