Main Article Content
phospholipase C, magnetic immobilisation, stability, oil degumming, 1,2-DAG
In this study, free phospholipase C (PLC) was immobilised onto magnetic carriers, including sodium alginate (MPCSA), magnetic chitosan microparticles (MCM), Fe3O4/P (glycidyl methacrylate (GMA)-EDGM-St) and Fe3O4/SiOx-g-P (GMA). The magnetic immobilised PLC had a broader pH activity and temperature tolerance, which could be separated quickly and reused. The highest enzyme loading and enzymatic activity (135.64 mg/g and 8,560 U/g, respectively) were obtained using the magnetic immobilised PLC-Fe3O4/SiOx-g-P (GMA) system. Its optimum pH range was 5.5-7.0, and activity remained stable between 50 and 70 °C. After 6 cycles in soybean lecithin emulsion, the magnetic immobilised enzyme retained more than 80% of its initial activity. Enzymatic degumming with PLC-Fe3O4/SiOx-g-P (GMA) was carried out 2.0 h at 60 °C, resulting in 1,2-diacylglycerol (1,2-DAG) content of 1.07%, which improving the refining rate of soybean oil. Moreover, PLC-Fe3O4/SiOx-g-P (GMA) still possessed more than 80% of its initial activity after 5 cycles of degumming in crude soybean oil. The results show that magnetic immobilised PLC has a good industrial application.
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