Main Article Content
hierarchical cluster analyses, principal component analyses, Salvia miltiorrhiza
Principal component analysis (PCA) and hierarchical cluster analysis (HCA) are quite useful chemometrics tools for the data analysis in many fields. In China P.R., Salvia miltiorrhiza Bunge is a traditional medicinal plant. Due to the unique action of its biologically active compounds in treating cardiovascular disease, S. miltiorrhiza was planted in many regions as the local economic crop. The roots, which are also called ‘Danshen’, were the original economical products. In this study, PCA and HCA were used to determine the quality of Danshen from two aspects, namely, agronomic traits and active compounds. PCA revealed that the first principal component cumulatively accounted for 50.1% of the total variance in the data set, while PC2 and PC3 explained 20.0 and 13.1%, respectively. The quality descending order of all samples obtained by PCA was as follows: tissue culture lobular Danshen, tissue culture broad leaf Danshen, short stalk and lobular leaf Danshen, broad leaf Danshen, lobular Danshen, and white leaf Danshen. Samples were classified into three main groups by HCA. Therefore, based on the provisions in the Chinese pharmacopoeia about the content of tanshinone IIA, ZD was considered the best line for the farmers. The research showed that PCA and HCA also could be used in the quality evaluation of medicinal crops.
Berrueta, L., Alonso-salces, R. and Héberger, K., 2007. Supervised pattern recognition in food analysis.Journal of Chromatography A 1158: 196-214.
Cheng, T.O., 2006. Danshen: a versatile Chinese herbal drug for the treatment of coronary heart disease. International Journal of Cardiology 113: 437-438.
China Pharmacopoeia Committee, 2010. Pharmacopoeia of the People’s Republic of China. Peoples Medicinal Publishing House, Beijing, China P.R., pp. 70.
Dharmara, S., Hossain, M., Zhari, S., Harn, G.L. and Ismail, Z., 2006. The use of principal component analysis and self-organizing map to monitor inhibition of calcium oxalate crystal growth by orthosiphon stamineus extract. Chemometrics and Intelligent Laboratory Systems 81: 21-28.
He, C.E., Wei, J.H., Jin, Y. and Chen, S.L., 2010. Bioactive components of the roots of Salvia miltiorrhizae: changes related to harvest time and germplasm line. Industrial Crops and Products 32: 313-317.
Kara, S., Güven, S. and Üven, S., 2007. Classification of macular and optic nerve disease by principal component analysis. Computers in Biology and Medicine 37: 836-841.
Kunal, R. and Gopinath, G., 2009. QSTR with extended topochemical atom (ETA) indices. 12. QSAR for the toxicity of diverse aromatic compounds to Tetrahymena pyriformis using chemometric tools. Chemosphere 77: 999-1009.
Leonard, S.T. and Droege, M., 2008. The uses and benefits of cluster analysis in pharmacy research. Research in Social and Administrative Pharmacy 4: 1-11.
Li, M.H., Chen, J.M., Peng, Y., Wu, Q.L. and Xiao, P.G., 2008. Investigation of Danshen and related medicinal plants in China. Journal of Ethnopharmacology 120: 419-426.
Li, L., Li, X.C., Wang, X.R. and Wang, S.W., 2003. Development of HPLC finger print barcode technique for authentication and quality assessment of radix Salvia miltiorrhiza. Chinese Traditional and Herbal Drugs 34: 649-653.
Li, Y.G., Song, L., Liu,M., Hu, Z.B. and Wang, Z.T., 2009. Advancement in analysis of Salviae miltiorrhizae Radix et Rhizoma (Danshen). Journal of Chromatography A 1216: 1941-1953.
Lima, D., Santos, A., Araujo, R., Scarminio, I., Bruns, R. and Ferreiraa, S., 2010. Principal component analysis and hierarchical cluster analysis for homogeneity evaluation during the preparation of a wheat flour laboratory reference material for inorganic analysis. Microchemical Journal 95: 222-226.
Liu, Y.R., Qu, S.X., Maitz, M.F., Tan, R. and Weng, J., 2007. The effect of the major components of Salvia miltiorrhiza Bunge on bone marrow cells. Journal of Ethnopharmacology 111: 573-583.
Liu, F.Y., Wang, X.D., Wang, S.H., Zou, Z.R. and Liang, Z.X., 2011. Effect of drought stress on growth and accumulation of active constituents in Salvia miltiorrhiza Bunge. Industrial Crops and Products 33: 84-88.
Matkowski, A., Zielin?ska, S., Oszmian?ski, J. and Lamer-zarawska, E., 2008. Activity of extracts from leaves and roots of Salvia miltiorrhizaBunge, S. przewalskii Maxim., and S. verticillata L. Bioresource Technology 99: 7892-7896.
Mebatsion, H.K., Paliwal, J. and Jayas, D.S., 2012. Evaluation of variations in the shape of grain types using principal components analysis of the elliptic Fourier descriptors. Computers and Electronics in Agriculture 80: 63-70.
Patras, A., Brunton, N., Downe, G., Rawson, A., Warriner, K. and Gernigon, G., 2011. Application of principal component and hierarchical cluster analysis to classify fruits and vegetables commonly consumed in Ireland based on in vitro antioxidant activity. Journal of Food Composition and Analysis 24: 250-256.
Shi, S.H., Wang, Q., Yuan, L.C. and Xu, H., 2006. Constituent determination and quality evaluation on resource plant species of Salvia L. in Yunnan. Journal of Plant Resources and Environment 15: 73-74.
Shu, Z.M., Liang, Z.S., Sun, Q., Ye, Z.L., Zhang, X.W. and Fu, L.L., 2007. Comparison and evaluation of biological traits in different Salvia miltiorrhiza germplasm. Journal of Xi’an University of Arts and Science 10: 24-29.
Wan, D.G., 2007. The varieties quality and potency of traditional Chinese herb. Shanghai Scientific and Technical Publishers, Shanghai, China P.R., pp 59.
Wang, W.Q., Peng, J., Wan, J., Fang, J.G., Tang, J. and Liu, Y.H., 2007. Chemical pattern recognition of quality assessment of Folium isatidis from various habitats. Chinese Traditional and Herbal Drugs 38: 921-925.
Zhang, X.G., Wang, Y.M., Luo, G.A. and Cheng, F.S., 2002. Studies on resource characteristics of Salvia miltiorrhiza varieties. Chinese Traditional and Herbal Drugs 33: 742-747.