Safety assessment of engineered metallic nanoparticles in foodstuff

Main Article Content

S. Rainieri
M. Olasagasti
A. Barranco

Keywords

analytical methods, food, metals, nanoparticles, toxicity

Abstract

The rapid proliferation of nanotechnology and the increasing use of nanomaterials and nanoparticles in many different fields (ranging from electronics, construction, medical and pharmaceutical products, cosmetics, household appliances, textile, motor vehicles, food, etc.), has led to the production of a variety of consumer products currently available on the market. In spite of such a widespread presence, the safety of such products for human health and for the environment has not yet been fully established. This issue is of particular concern for the food sector, especially for metallic nanoparticles that are not biocompatible and tend to accumulate in the organism. These concerns seem to arise from the current lack of knowledge of the potential toxic effects of engineered nanoparticles on human and environmental health as well as from the lack of standardised methodologies to test such effects and the lack of specific regulations. This review presents an overview of the current way to assess the safety of nanoparticles and nanotechnological applications, with a special focus on metallic engineered nanoparticles and their application in the food sector. Specifically, it illustrates the methodological gaps and as well as the current regulatory and ethical issues related to this branch of technology.

Abstract 279 | PDF Downloads 285

References

Arnaud, I., Abid, J.P., Roussel, C. and Girault, H.H., 2005. Size-selective separation of gold nanoparticles using isoelectric focusing electrophoresis (IEF). Chemical Communications 6: 787-788.
Arora, S., Rajwade, J.M. and Paknikar, K.M., 2012. Nanotoxicology and in vitro studies: the need of the hour. Toxicology and Applied Pharmacology 258: 151-165.
Asharani, P.V., Lianwu, Y., Gong, Z. and Valiyaveettil, S., 2010. Comparison of the toxicity of silver, gold and platinum nanoparticles in developing zebrafish embryos. Nanotoxicology 5: 43-54.
Asharani, P.V., Wu, Y.L., Gong, Z. and Valiyaveettil, S., 2008. Toxicity of silver nanoparticles in zebrafish models. Nanotechnology 19: 1-8.
Balnois, E., Papastavrou, G. and Wilkinson, K.J., 2007. Force microscopy and force measurements of environmental colloids. In: Wilkinson, K.J. and Lead, J.R. (eds.) Environmental colloids and particles: behaviour, structure and characterization. John Wiley and Sons, Chichester, UK, pp. 405-468.
Bandyopadhyay, S., Peralta-Videa, J.R. and Gardea-Torresdey, J.L., 2013. Advanced analytical techniques for the measurement of nanomaterials in food and agricultural samples: a review. Environmental Engineering Science 30: 118-125.
Beer, C., Foldbjerg, R., Hayashi, Y., Sutherland, D.S. and Autrup, H., 2012. Toxicity of silver nanoparticles-nanoparticle or silver ion? Toxicology Letters 208: 286-292.
Beltrami, D., Calestani, D., Maffini, M., Suman, M., Melegari, B., Zappettini, A. and Mangia, A., 2011. Development of a combined SEM and ICP-MS approach for the qualitative and quantitative analyses of metal microparticles and sub-microparticles in food products. Analytical and Bioanalytical Chemistry 401: 1401-1409.
Blasco, C. and Picó, Y., 2011. Determining nanomaterials in food. Trends in Analytical Chemistry 30: 84-99.
Bohnsack, J.P., Assemi, S., Miller, J.D. and Furgeson, D.Y., 2012. The primacy of physicochemical characterization of nanomaterials for reliable toxicity assessment: a review of the zebrafish nanotoxicology model. Methods in Molecular Biology 926: 261-316.
Bouwmeester, H., Dekkers, S., Noordam, M.Y., Hagens, W.I., Bulder, A.S., De Heer, C., Ten Voorde, S.E., Wijnhoven, S.W., Marvin, H.J. and Sips, A.J., 2009. Review of health safety aspects of nanotechnologies in food production. Regulatory Toxicology and Pharmacology 53: 52-62.
Bouwmeester, H., Poortman, J., Peters, R.J., Wijma, E., Kramer, E., Makama, S., Puspitaninganindita, K., Marvin, H.J., Peijnenburg, A.A. and Hendriksen, P.J., 2011. Characterization of translocation of silver nanoparticles and effects on whole-genome gene expression using an in vitro intestinal epithelium coculture model. ACS Nano 24: 4091-4103.
Brar, S.K. and Verma, M., 2011. Measurement of nanoparticles by light-scattering techniques. Trends in Analytical Chemistry 30: 4-17.
Braun, A., Kestens, V., Franks, K., Roebben, G., Lamberty, A. and Linsinger, T.P.J., 2012. A new certified reference material for size analysis of nanoparticles. Journal of Nanoparticle Research 14: 1-12.
Busolo, M.A., Fernandez, P., Ocio, M.J. and Lagaron, J.M., 2010. Novel silver-based nanoclay as an antimicrobial in polylactic acid food packaging coatings. Food Additives and Contaminants: Part A, Chemistry, Analysis, Control, Exposure and Risk Assessment 27: 1617-1626.
Cai, S.J., Wu, C.X., Gong, L.M., Song, T., Wu, H. and Zhang, L.Y., 2012. Effect of nano-selenium on performance, meat quality, immune function, oxidation resistance, and tissue selenium content in broilers. Poultry Science 91: 2532-2539.
Card, J.W., Jonaitis, T.S., Tafazoli, S. and Magnuson, B.A., 2011. An appraisal of the published literature on the safety and toxicity of food-related nanomaterials. Critical Reviews in Toxicology 41: 22-49.
Card, J.W. and Manguson, B., 2007. Proposed minimum characterization parameters for studies on food and food-related nanomaterials. Journal of Food Science 74: vi-vii.
Carney, R.P., Kim, J.Y., Qian, H., Jin, R., Mehenni, H., Stellacci, F. and Bakr, O.M., 2011. Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation. Nature Communication 2: 335.
Chae, Y.J., Lee, J., Bae, E., Yi. J. and Gu, M.B., 2009. Evaluation of the toxic impact of silver nanoparticles on Japanese medaka (Oryzias latipes). Aquatic Toxicology 94: 320-327.
Chaudhry, Q., Castle, L. and Watkins, R., 2010. Nanotechnologies in food. RSC Publishing, Cambridge, UK.
Chaudhry, Q. and Groves, K., 2010. Nanotechnology applications for food ingredients, additives and supplements. In: Chaudhry, Q., Castle, L. and Watkins, R. (eds.) Nanotechnologies and food. RCS Publishing, Cambridge, UK, pp. 69-84.
Chaudhry, Q., Scotter, M., Blackburn, J., Ross, B., Boxall, A., Aitken, R. and Watkins, R., 2008. Applications and implications of nanotechnologies for the food sector. Food Additives and Contaminants: Part A 25: 241-258.
Chen, X.X., Cheng, B., Yan, Y.X., Cao, A., Liu, J.H., Du, L.J, Liu Y., Zhao, Y. and Wang, H., 2013. Characterization and preliminary toxicity assay of nano-titanium dioxide additive in sugar-coated chewing gum. Small 9: 1765-1774.
Choi, J.E., Kim, S., Ahn, J.H., Youn, P., Kang, J.S., Park, K., Yi, J. and Ryu, D.Y., 2010b. Induction of oxidative stress and apoptosis by silver nanoparticles in the liver of adult zebrafish. Aquatic Toxicology 100: 151-159.
Choi, J.H., Shin, J., Lim, S.Y., Oh, J.M., Oh, M.H. and Oh, S., 2010a. Characterization and stability analysis of zinc oxide nano encapsulated conjugated linoleic acid. Journal of Food Science 75: N63-N68.
Coles, D. and Frewer, L.J., 2013. Nanotechnology applied to European food production – a review of ethical and regulatory issues. Trends in Food Science and Technology 34: 32-43.
Conte, A., Longano, D., Costa, C., Ditaranto, N., Ancona, A., Cioffi, N., Scrocco, C., Sabbatini, L., Contó, F. and Del Nobile, M.A., 2013. A novel preservation technique applied to fiortilatte cheese. Innovative Food Science and Emerging Technologies 19: 158-165.
Donaldson, K., Stone, V., Clouter, A., Renwick, L. and MacNee, W., 2001. Ultrafine particles. Occupational and Environmental Medicine 58: 211-216.
Donaldson, K., Stone, V., Tran, C.L., Kreyling, W. and Borm, P.J., 2004. Nanotoxicology. Occupational and Environmental Medicine 61: 727-728.
Dudkiewicz, A., Tiede, K., Loeschner, K., Jensen, L.H.S., Jensen, E., Wierzbicki, R., Boxall, A.B.A. and Molhave, K., 2011. Characterization of nanomaterials in food by electron microscopy. Trends in Analytical Chemistry 30: 28-43.
Duncan, T.V., 2011. Applications of nanotechnology in food packaging and food safety: barrier materials, antimicrobials and sensors. Journal of Colloid and Interface Science 363: 1-24.
Embry, M.R., Belanger, S.E., Braunbeck, T.A., Galay-Burgos, M., Halder, M., Hinton, D.E., Léonard, M.A., Lillicarp, A., Norberg-King, T. and Whale, G., 2010. The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research. Aquatic Toxicology 97: 79-87.
European Commission (EC), 1997. Commission regulation (EC) no. 258/97 of the European Parliament and of the Council of 27 January 1997 concerning novel foods and novel food ingredients. Official Journal of the European Union L43: 1-9.
European Commission (EC), 2002. Commission Regulation (EC) no. 178/2002 of the European Parliament and of the Council of 28 January 2002 laying down the general principles and requirements of food law, establishing the European Food Safety Authority and laying down procedures in matters of food safety. Official Journal of the European Union L31: 1-40.
European Commission (EC), 2004. Commission regulation (EC) no. 1935/2004 of the European Parliament and of the Council of 27 October 2004 on materials and articles intended to come into contact with food and repealing Directives 80/590/EEC and 89/109/EEC. Official Journal of the European Union L338: 4-17.
European Commission (EC), 2008. Commission regulation (EC) no. 1333/2008 of the European Parliament and of the Council of 16 December 2008 on food additives. Official Journal of the European Union L354: 16-33.
European Commission (EC), 2011. Commission regulation (EC) no. 1169/2011 of the European Parliament and of the Council of 25 October 2011 on the provision of food information to consumers, amending regulation (EC) no. 1924/2006 and (EC) no. 1925/2006 of the European Parliament and of the Council, and repealing Commission Directive 87/250/EEC, Council Directive 90/496/EEC, Commission Directive 1999/10/EC, Directive 2000/13/EC of the European Parliament and of the Council, Commission Directives 2002/67/EC and 2008/5/EC and Commission regulation (EC) no.608/2004.Official Journal of the European Union L304: 18-89.
European Food Safety Authority (EFSA), 2011. Guidance on the risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain. EFSA Journal 9: 2140.
Fabrega, J., Luoma, S.N., Tyler, C.R., Galloway, T.S. and Lead, J.R., 2011. Silver nanoparticles: behaviour and effects in the aquatic environment. Environment international 37: 517-531.
Fernandez, A., Lloret, E., Llorens, A. and Picouet, P., 2012. Metal-based mciro and nano-composites as antimicrobials in food packaging. In: Kontominas M.G. (ed.) Food packaging: procedures, management and trends. Nova Science Pub Inc., Hauppauge, NY, USA, pp.79-92.
Fernández, B., Costa, J.M., Pereiro, R. and Sanz-Medel, A., 2010. Inorganic mass spectrometry as a tool for characterisation at the nanoscale. Analytical and Bioanalytical Chemistry 396: 15-29.
Fischer, H.C. and Chan, W.C.W., 2007. Nanotoxicity: the growing need for in vivo study. Current Opinion in Biotechnology 18: 565-571.
Food and Drug Administration (FDA), 2012. Draft guidance on ‘assessing the effects of significant manufacturing process changes, including emerging technologies, on the safety and regulatory status of food ingredients and food contact substances (FCSs), including food ingredients that are color additives’. FDA, Silver Spring, MD, USA. Available at: http://tinyurl.com/bnuefuc.
Gallego-Urrea, J.A., Tuoriniemi, J. and Hassellöv, M., 2011. Applications of particle-tracking analysis to the determination of size distributions and concentrations of nanoparticles in environmental, biological and food samples. Trends in Analytical Chemistry 30: 473-483.
Gatti, A.M., Tossini, D., Gambarelli, A., Montanari, S. and Capitani, F., 2009. Investigation of the presence of inorganic micro- and nanosized contaminants in bread and biscuits by environmental scanning electron microscopy. Critical Reviews in Food Science and Nutrition 49: 275-282.
Griffitt, R.J., Lu, J., Gao, J., Bonzongo, J.C. and Barber, D.S., 2008. Effects of particle composition and species on toxicity of metallic nanomaterials in aquatic organisms. Environmental Toxicolology and Chemistry 27: 1972-1978.
Grobe, A. and Rissanen, M.E., 2012. Nanotechnologies in agriculture and food – an overview of different fields of application, risk assessment and public perception. Recent Patent on Food Nutrition and Agriculture 4: 176-186.
Guan, B., Lu, W., Fang, J. and Cole, R.B., 2007. Characterization of synthesized titanium oxide nanoclusters by MALDI-TOF mass spectrometry. Journal of the American Society for Mass Spectrometry 18: 517-524.
Gutierrez, F.J., Albillos, S.M., Casas-Sanz, E., Cruz, Z., Garcia-Estrada, C., Garcia-Guerra, A., Garcia-Reverter, J., Garcia-Suarez, M., Gaton, P., Gonzalez-Ferrero, C., Olabarrieta, I., Olasagasti, M., Rainieri, S., Rivera-Patño, D., Rojo, R., Romo-Hualde, A., Saiz-Abajo, M.J. and Mussons, M.L., 2013. Methods for the nanoencapsulation of beta-carotene in the food sector. Trends in Food Science and Technology 32: 73-83.
Hagendorfer, H., Kaegi, R., Traber, J., Mertens, S.F., Scherrers, R., Ludwig, C. and Ulrich, A., 2011. Application of an asymmetric flow field flow fractionation multi-detector approach for metallic engineered nanoparticle characterization--prospects and limitations demonstrated on Au nanoparticles. Analytica Chimica Acta 706: 367-378.
Hansen, S.F. and Baun, A., 2012. European regulation affecting nanomaterials – review of limitations and future recommendations. Dose-Response 10: 364-383.
Hassellow, M., Readman, J.W., Ranville, J.F. and Tiede, K., 2008. Nanoparticle analysis and characterization methodologies in environmental risk assessment of engineered nanoparticles. Ecotoxicology 17: 344-361.
Helfrich, A. and Bettmer, J., 2011. Analysis of gold nanoparticles using ICP-MS-based hyphenated and complementary ESI-MS techniques. International Journal of Mass Spectrometry 307: 92-980.
Ho, K.S., Lui, K.O., Lee, K.H. and Chan, W.T., 2013. Considerations of particle vaporization and analyte diffusion in single-particle inductively coupled plasma-mass spectrometry. Spectrochimica Acta Part B 89: 30-39.
Jiang, J., Oberdörster, G., Elder, A., Gelein, R., Mercer, P. and Biswas, P., 2008. Does nanoparticle activity depend upon size and crystal phase? Nanotoxicology 2: 33-42.
Jiang, T., Feng, L. and Wang, Y., 2013. Effect of alginate/nano-Ag coating on microbial and physicochemical characteristics of shiitake mushroom (Lentinus edodes) during cold storage. Food Chemistry 141: 954-960.
Kammer, F.V.D., Legros, S., Hofmann, T., Larsen, E.H. and Loeschner, K., 2011. Separation and characterization of nanoparticles in complex food and environmental samples by field-flow fractionation. Trends in Analytical Chemistry 30: 425-436.
Kawata, K., Osawa, M. and Okabe, S., 2009. In vitro toxicity of silver nanoparticles at noncytotoxic doses to HepG2 human hepatoma cells. Environmental Science and Technology 43: 6046-6051.
Khurana, C., Vala, A.K., Andhariya, N., Pandey, O.P. and Chudasama, B., in press. Antibacterial activity of silver: the role of hydrodynamic particle size at nanoscale. Journal of Biomedical Material Research Part A, DOI: http://dx.doi.org/10.1002/jbm.a.35005.
Kim, S., Choi, J.E., Choi, J., Chung, K.H., Park, K., Yi, J. and Ryu, D.Y., 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology in Vitro23: 1076-1084.
Kim, S., Marion, M., Jeong, B.H. and Hoek, E., 2006. Crossflow membrane filtration of interacting nanoparticle suspensions. Journal of Membrane Science 284: 361-372.
Kim, S. and Ryu, D.Y., 2013. Silver nanoparticle-induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues. Journal of Applied Toxicology 33: 78-89.
Koch, M., Kiefer, S., Cavelius, C. and Kraegeloh, A., 2012. Use of a silver ion selective electrode to assess mechanisms responsible for biological effects of silver nanoparticles. Journal of Nanoparticle Research 14: 646-657.
Kowalczyk, B., Lagzi, I. and Grzybowski, B.A., 2011. Nanoseparations: strategies for size and/or shape-selective purification of nanoparticles. Current Opinion in Colloid and Interface Science 16: 135-148.
Laban, G., Nies, L.F., Turco, R.F., Bickham, J.W. and Sepúlveda, M.S., 2010. The effects of silver nanoparticles on fathead minnow (Pimephales promelas) embryos. Ecotoxicology19: 185-195.
Lacava, L.M., Lacava, B.M., Azevedo, R.B., Lacava, Z.G.M., Buske, N., Tronconi, A.L. and Morais, P.C., 2001. Nanoparticle sizing: a comparative study using atomic force microscopy, transmission electron microscopy, and ferromagnetic resonance. Journal of Magnetism and Magnetic Materials 225: 79-83.
Lei, S.G., Hoa, S.V. and Ton-That, M.T., 2006. Effects of clay types on the processing properties of polypropylene nanocomposites. Composites Science and Technology 66: 1274-1279.
Linsinger, T.P.J., Chaudhry, Q., Dehalu, V., Delahaut, P., Dudkiewicz, A., Grombe, R., Von der Kammer, F., Larsen, E.H., Legros, S., Loeschner, K., Peters, R., Ramsch, R., Roebben, G., Tiede, K. and Weigel, S., 2013. Validation of methods for the detection and quantification of engineered nanoparticles in food. Food Chemistry 138: 1959-1966.
Liu, J.F., Yu, S.J., Yin, Y.G. and Chao, J.B., 2012. Methods for separation, identification, characterization and quantification of silver nanoparticles. Trends in Analytical Chemistry 33: 95-106.
Llorens, A., Lloret, E., Picouet, P.A., Trbojevich, R. and Fernandez, A., 2012. Metallic-based micro and nanocomposites in food contact materials and active food packaging. Trends in Food Science and Technology 24 19-29.
López-Lorente, A.I., Simonet, B.M. and Valcárcel M., 2011. Electrophoretic methods for the analysis of nanoparticles. Trends in Analytical Chemistry 30: 58-71.
Luo, P., Morrison, I., Dudkiewicz, A., Tiede, K., Boyes, E., O’Toole, P., Park, S. and Boxall, A.B., 2013. Visualization and characterization of engineered nanoparticles in complex environmental and food matrices using atmospheric scanning electron microscopy. Journal of Microscopy 250: 32-41.
Luoma, S.N., 2008. Silver nanotechnologies and the environment: old problems or new challenges? Woodrow Wilson International Center for Scholars and Pew Charitable Trust, Washington, DC, USA. Available at: http://www.nanotechproject.org/process/assets/files/7036/nano_pen_15_final.pdf.
Majedi, S.M, Kelly, B.C. and Lee, H.K., 2013. Efficient hydrophobization and solvent microextraction for determination of trace nano-sized silver and titanium dioxide in natural waters. Analytica Chimica Acta 789: 47-57.
Malloy, A. and Carr, B., 2006. Nanoparticle tracking analysis – the HALO system. Particle and Particle Systems Characterization 23: 197-204.
Maynard, A.D., Warheit, D.B. and Philbert, M.A., 2011. The new toxicology of sophisticated materials: nanotoxicology and beyond. Toxicological Sciences 120: S109-S129.
Mitrano, D.M., Lesher, E.K., Bednar, A., Monserud, J., Higgins, C.P. and Ranville, J.F., 2012. Detecting nanoparticulate silver using single-particle inductively coupled plasma-mass spectrometry. Environmental Toxicology and Chemistry 31: 115-121.
Murdock, R.C., Braydich-Stolle, L., Schrand, A.M., Schlager, J.J. and Hussain, S.M., 2008. Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicological Sciences 101: 239-253.
Naddy, R.B., Gorsuch, J.W., Rehner, A.B., McNerney, G.R., Bell, R.A. and Kramer, J.R., 2007. Reprint of ‘chronic toxicity of silver nitrate to Ceriodaphnia dubia and Daphnia magna, and potential mitigating factors’. Aquatic Toxicology 84: I-X.
Navarro, E., Piccapietra, F., Wagner, B., Marconi, F., Kaegi, R., Odzak, N., Sigg, L. and Behra R., 2008. Toxicity of silver nanoparticles to Chlamydomonas reinhardtii. Environmental Science and Technology 42: 8959-8964.
Oberdörster, G., 2010. Safety assessment for nanotechnology and nanomedicine: concepts of nanotoxicology. Journal of Internal Medicine 267: 89-105.
Oberdörster, G., Maynard, A., Donaldson, K., Castranova, V., Fitzpatrick, J., Carter, J., Karn, B., Kreyling, W., Lai, D., Olin, S., Monteiro-Riviere, N., Warheit, D. and Yang, H., 2005. Principles for characterizing the potential human health effects from exposure to nanomaterials: elements of a screening strategy. Particles and Fibre Toxicology 6: 2-8.
Olasagasti, M., Gatti, M.A., Capitani, F., Barranco, A., Pardo, M.A., Escuredo, K. and Rainieri, S., 2014. Toxic effects of colloidal nanosilver in zebrafish embryos. Journal of Applied Toxicology 34: 562-575.
Olesik, J.W. and Gray, P.J., 2012. Considerations for measurement of individual nanoparticles or microparticles by ICP-MS: determination of the number of particles and the analyte mass in each particle. Journal of Analytical Atomic Spectrometry 27: 1143-1155.
Park, M.V.D.Z., Neigh, A.M., Vermeulen, J.P., De la Fonteyne, L.J.J., Verharen, H.W., Briedé, J.J., Van Loveren, H. and De Jon, W.H., 2011. The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials 32: 9810-9817.
Pérez, S., Farré, M. and Barceló, D., 2009. Analysis, behavior and ecotoxicity of carbon-based nanomaterials in the aquatic environment. Trends in Analytical Chemistry 28: 820-832.
Pérez-Esteve, E., Bernardos, A., Martinez-Máñez, R. and Barat, J.M., 2013. Nanotechnology in the development of novel functional foods or their package. An overview based in patent analysis. Recent Patent on Food Nutrition and Agriculture 5: 35-43.
Pergantis, S.A., Jones-Lepp, T.L. and Heithmar, E.M., 2012. Hydrodynamic chromatography online with single particle-inductively coupled plasma mass spectrometry for ultratrace detection of metal-containing nanoparticles. Analytical Chemistry 84: 6454-6462.
Peters, R., Dam, G.T., Bouwmeester, H., Helsper, H., Allmaier, G., Kammer, F.V., Ramsch, R., Solans, C., Tomaniová, M., Hajslova, J. and Weigel, S., 2011. Identification and characterization of organic nanoparticles in food. Trends in Analytical Chemistry 30: 100-112.
Poda, A.R., Bednar, A.J., Harmon, A., Hull, M. and Mitrano, D.M., 2010. Characterization of silver nanoparticles using flow-field flow fractionation interfaced to inductively coupled plasma mass spectrometry. Journal of Chromatography 1218: 4219-4225.
Project on Emerging Nanotechnologies, 2014. Consumer products inventory. Project on Emerging Nanotechnologies, Washington, DC, USA. Available at: http://www.nanotechproject.org/cpi.
Raz, S.R., Leontaridou, M., Bremer, M.G.E.G., Peters, R. and Weigel, S., 2012. Development of surface plasmon resonance-based sensor for detection of silver nanoparticles in food and the environment. Analytical and Bioanalytical Chemistry 403: 2843-2850.
Royal Society and the Royal Academy of Engineering (RS/RAE), 2004. Nanoscience and nanotechnologies: opportunities and uncertainties. RS, London, UK. Available at: http://www.nanotec.org.uk/finalReport.htm.
Rushton, E.K., Jiang, J., Leonard, S.S., Eberly, S., Castranova, V., Biswas, P., Elder, A., Han, X., Gelein, R., Finkelstein, J. and Oberdörster, G., 2010. Concept of assessing nanoparticles hazards considering nanoparticle dosemetric and chemical/biological response metrics. Journal of Toxicology and Environmental Health Part A 73: 445-461.
Sadik, O.A., Zhou, A.L., Kikandi, S., Du, N., Wang, Q. and Varner, K., 2009. Sensors as tools for quantitation, nanotoxicity and nanomonitoring assessment of engineered nanomaterials. Journal of Environmental Monitoring 11: 1782-1800.
Sánchez-Pomales, G., Mudalige, T.,K., Lim, J.,H. and Linder, S.W., 2013. Rapid determination of silver in nanobased liquid dietary supplements using a portable x-ray fluorescence analyser. Journal of Agricultural and Food Chemistry 61: 7250-7257.
Sayes, C.M., Reed, K.L. and Warheit, D.B., 2007. Assessing toxicology on fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxic profiles. Toxicological Sciences 97: 163-180.
Scheffer, A., Engelhard, C., Sperling, M. and Buscher, W., 2008. ICP-MS as a new tool for the determination of gold nanoparticles in bioanalytical applications. Analytical and Bioanalytical Chemistry 390: 249-252.
Schmidt, B., Loeschner, K., Hadrup, N., Mortensen, A., Sloth, J.J., Koch, C.B. and Larsen, E.H., 2011. Quantitative characterization of gold nanoparticles by field-flow fractionation coupled online with light scattering detection and inductively coupled plasma mass spectrometry. Analytical Chemistry 83: 2461-2468.
Senior, K., Müller, S., Schacht, V.J. and Bunge, M., 2012. Antimicrobial precious-metal nanoparticles and their use in novel materials. Recent Patents on Food Nutrition and Agriculture 4: 200-209.
Shaw, B.J. and Handy, R.D., 2011. Physiological effects of nanoparticles on fish: a comparison of nanometals versus metal ions. Environmenal International 37: 1083-1097.
Shi, L.E., Li, Z.H., Zheng, W., Zhao, Y.F., Jin, Y.F. and Tang Z.X., 2014. Synthesis, antibacterial activity, antibacterial mechanism and food applications of ZnO nanoparticles: a review. Food Additives and Contaminants – Part A 31: 173-186.
Silbergeld, E.K., Contreras, E.Q., Hartung, T., Hirsch, C., Hogberg, H., Jachak, A.C., Jordan, W., Landsiedel, R., Morris, J., Patri, A., Pounds, J.G., De Vizcaya Ruiz A., Shvedova, A., Tanguay, R., Tatarazako, N., Van Vliet, E., Walker, N.J., Wiesner, M., Wilcox, N. and Zurlo, J., 2011. T4 workshop report. Nanotoxicology: ‘the end of the beginning’ – signs on the roadmap to a strategy for assuring the safe application and use of nanomaterials. Altex 28: 236-241.
Silva, B.F., Pérez, S., Gardinalli, P., Singhal, R.K., Mozeto, A.A. and Barceló, D., 2011 Analytical chemistry of metallic nanoparticles in natural environments. Trends in Analytical Chemistry 30: 528-540.
Stone, V., Johnston, H. and Clift, M.J., 2007. Air pollution, ultrafine and nanoparticle toxicology: cellular and molecular interactions. IEEE Trans Nanobioscience 6: 331-340.
Strähle, U., Scholz, S., Geisler, R., Greiner, P., Hollert, H., Rastegar, S., Schumacher, A., Selderslaghs, I., Weiss, C., Witters, H. and Braunbeck, T., 2012. Zebrafish embryos as an alternative to animal experiments – a commentary on the definition of the onset of protected life stages in animal welfare regulations. Reproductive Toxicology 33: 128-132.
Sung, J.H., Ji, J.H., Park, J.D., Yoon, J.U., Kim, D.S., Jeon, K.S., Song, M.Y., Jeong, J., Han, B.S., Han, J.H., Chung, Y.H., Chang, H.K., Lee, J.H., Cho, M.H,, Kelman, B.J. and Yu, I.J., 2009. Subchronic inhalation toxicity of silver nanoparticles. Toxicological Sciences 108: 452-461.
Teeguarden, J.G., Hinderliter, P.M., Orr, G., Thrall, B.D. and Pounds, J.G., 2007. Particokintetics in vitro: dosimetry consideration forin vitro nanoparticle toxicity assessments. Toxicological Sciences 95: 300-312.
Tiede, K., Boxall, A.B.A., Tear, S.P., Lewis, J., David, H. and Hassellov, M., 2008. Detection and characterization of engineered nanoparticles in food and the environment. Food Additives and Contaminants Part A: 25: 795-821.
Tsuji, J.S., Maynard, A.D., Howard, P.C., James, J.T., Lam, C.W., Warheit, D.B. and Santamaria, A.B., 2006. Research strategies for safety evaluation for nanomaterials, part IV: risk assessment of nanoparticles. Toxicological Sciences 89: 42-50.
Tuoriniemi, J., Cornelis, G. and Hassellov, M., 2012. Size discrimination and detection capabilities of single-particle ICPMS for environmental analysis of silver nanoparticles. Analytical Chemistry 84: 3965-3972.
Ungvári, E., Monori, I., Megyeri, A., Csiki, Z., Prokisch, J., Sztrik, A., Jávor, A. and Benkö, I., 2014. Protective effects of meat from lambs on selenium nanoparticle supplemented diet in a mouse model of polycyclic aromatic hydrocarbon-induced immunotoxicity. Food and Chemical Toxicology 64: 298-306.
Wang, H., Du, L.J., Song, Z.M. and Chen, X.X., 2013. Progress in the characterization and safety evaluation of engineered inorganic nanomaterials in food. Nanomedicine 8: 2007-2025.
Warheit, D.B., 2008. How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization? Toxicological Sciences 101: 183-185.
Warheit, D.B., Webb, T.R., Sayes, C.M., Colvin, V.L. and Reed, K.L., 2006. Pulmonary instillation studies with nanoscale TiO2 rods and dots in rats: toxicity is not dependent upon particle size and surface area. Toxicological Sciences 91: 227-236.
Weir, A., Westerhoff, P., Fabricius, L., Hristovski, K. and Von Goetz, N., 2012. Titanium dioxide nanoparticles in food and personal care products. Environmental Science and Technology 46: 2242-2250.
Wohlleben, W., 2012. Validity range of centrifuges for the regulation of nanomaterials: from classification to as-tested coronas. Journal of Nanoparticle Research 14: 1-18.
Wu, N., Wyart, Y., Liu, Y., Rose, J. and Moulin, P., 2013. An overview of solid/liquid separation methods and size fractionation techniques for engineered nanomaterials in aquatic environment. Environmental Technology 2: 1-16.
Zanni, E., De Bellis, G., Bracciale, M.P., Broggi, A., Santarelli, M.L., Sarto, M.S., Palleschi, C. and Uccelletti, U., 2012. Graphite nanoplatelets and Caenohabditis elegans: insight from a in vivomodel. Nano Letters 12: 2740-2744.
Zattoni, A., Roda, B., Borghi, F., Marassi, V. and Reschiglian, P., 2014 Flow field-flow fractionation for the analysis of nanoparticles used in drug delivery. Journal of Pharmaceutical and Biomedical Analysis 87: 53-61.
Zhao, C.M. and Wang, W.X., 2011. Comparison of acute and chronic toxicity of silver nanoparticles and silver nitrate to Daphnia magna. Environmental Toxicology and Chemistry 30: 885-92.