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4 EU marker PAHs, Turkish yogurt, yogurt, polycyclic aromatic hydrocarbons, HPLC
The level of 4 EU marker polycyclic aromatic hydrocarbons (4PAHs) was determined in various types of yogurt samples consumed in Turkey. Seventeen kinds of retail yogurt were purchased from supermarkets and local dairy firms in Turkey. A high-performance liquid chromatography method involving a liquid-liquid extraction and a pre-concentration step was applied for the determination of 4PAHs. The method was validated according to single laboratory validation guidelines with parameters such as selectivity, precision, limit of detection, limit of quantitation, linearity, accuracy, and also measurement uncertainty. The highest concentration for sum of the 4PAHs was found 0.59 ?g/kg and 0.95 ?g/kg in yogurt samples with low and high fat content, respectively. The present paper is the first study concerning the levels of polycyclic aromatic hydrocarbons in yogurts from Turkey.
Aguinaga, N., Campillo, N., Viñas, P. and Hernández-Córdoba, M., 2007. Determination of 16 polycyclic aromatic hydrocarbons in milk and related products using solid-phase microextraction coupled to gas chromatography-mass spectrometry. Analytica Chimica Acta 596(2): 285-290.
Bansal, V. and Kim, K., 2015. Review of PAH contamination in food products and their health hazards. Environment International 84: 26-38.
Bansal, V., Kumar, P., Kwon, E.E. and Kim, K.H., 2017. Review of the quantification techniques for Polycyclic Aromatic Hydrocarbons (PAHs) in food products. Critical Reviews in Food Science and Nutrition 57(15): 3297-3312.
Barranco, A., Alonso-Salces, R.M., Bakkali, A., Berrueta, L.A., Gallo, B., Vicente, F. and Sarobe, M., 2003. Solid-phase clean-up in the liquid chromatographic determination of polycyclic aromatic hydrocarbons in edible oils. Journal of Chromatography A 988(1): 33-40.
Battisti, C., Girelli, A.M. and Tarola, A.M., 2015. Polycyclic Aromatic Hydrocarbons (PAHs) in yogurt samples. Food Additives & Contaminants: Part B 8(1): 50-55.
Chung, T.L., Liao, C.J. and Chen, M.F., 2010. Comparison of liquid-liquid extraction and solid-phase extraction for the determination of polycyclic aromatic hydrocarbons in the milk of Taiwan. Journal of the Taiwan Institute of Chemical Engineers 41(2): 178-183.
Citac and Eurachem, 2000. EURACHEM/CITAC guide CG- quantifying uncertainty in analytical measurement, 2nd edition, 126 pp.
Codex Committee on Food Additives and Contaminants (CCFAC), 2005. Discussion paper on Polycyclic Aromatic Hydrocarbons (PAH) contamination. Joint FAO/WHO Food Standards Programme 17(October 2004). FAO/WHO, Rome/Geneva, Italy/Switzerland.
Eurachem, 2014. Eurachem guide: the fitness for purpose of analytical methods – a laboratory guide to method validation and related topics. Available at: https://www.eurachem.org/images/stories/Guides/pdf/MV_guide_2nd_ed_EN.pdf
European Commission (EC), 2005. Commission Recommendation of 2005/108 of 4 February 2005 on the further investigation into the levels of polycyclic aromatic hydrocarbons in certain foods. Official Journal of the European Union L34(208): 43-45.
European Commission (EC), 2011a. Commission Regulation (EU) No 835/2011 of 19 August 2011 amending Regulation (EC) No 1881/2006 as regards maximum levels for polycyclic aromatic hydrocarbons in foodstuffs. Official Journal of the European Union 215: 4-8.
European Commission (EC), 2011b. Commission Regulation (EU) No 836/2011. Official Journal of the European Union L 215/9: 9-16. Available at: https://tinyurl.com/y3p8rpz7
European Commission Scientific Committee on Food (SCF), 2002a. Opinion of the scientific committee on food on the risks to human health of polycyclic aromatic hydrocarbons in food, pp. 1-84. Available at: http://tinyurl.com/yxdj566x
European Commission Scientific Committee on Food (SCF), 2002b. Polycyclic aromatic hydrocarbons – occurrence in foods, dietary exposure and health effects. Annex A1-194. Available at: http://tinyurl.com/yyeqa2n5
European Food Safety Authority (EFSA), 2008a. Findings of the EFSA data collection on polycyclic aromatic hydrocarbons in food. EFSA Journal 724: 1-55.
European Food Safety Authority (EFSA), 2008b. Polycyclic aromatic hydrocarbons in food. scientific opinion of the panel on contaminants in the food chain. EFSA Journal 724: 1-1144.
Falcó, G., Domingo, J.L., Llobet, J.M., Teixidó, A., Casas, C. and Müller, L., 2003. Polycyclic aromatic hydrocarbons in foods: human exposure through the diet in Catalonia, Spain. Journal of Food Protection 66(12): 2325-2331.
Food and Agriculture Organisation/World Health Organisation (FAO/WHO), 2005. Joint FAO/WHO expert Committee on Food Additives (JECFA). Summary and conclusions of the 64th meeting, pp. 1-47. Available at: http://www.fao.org/3/a-at877e.pdf
Girelli, A.M., Sperati, D. and Tarola, A.M., 2014. Determination of polycyclic aromatic hydrocarbons in Italian milk by HPLC with fluorescence detection. Food Additives & Contaminants: Part A 31(4): 703-710.
International Agency for Research on Cancer (IARC), 2010. Some non-heterocyclic polycyclic aromatic hydrocarbons and some related exposures. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 92: 1-868.
International Agency for Research on Cancer (IARC), 2012. A review of human carcinogens: chemical agents and related occupations. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans 100F: 225-248.
Jira, W., Ziegenhals, K. and Speer, K., 2008. Gas chromatography-mass spectrometry (GC-MS) method for the determination of 16 European priority polycyclic aromatic hydrocarbons in smoked meat products and edible oils. Food Additives & Contaminants: Part A 25(6): 704-713.
Kacmaz, S., 2016. Polycyclic aromatic hydrocarbons in cereal products on the Turkish market. Food Add?t?ves & Contam?nants: Part B 9(3): 191-197.
Kacmaz, S., Zelinkova, Z. and Wenzl, T., 2016. Rapid and sensitive method for the determination of four EU marker polycyclic aromatic hydrocarbons in cereal-based foods using isotope-dilution GC/MS. Food Additives and Contaminants – Part A Chemistry, Analysis, Control, Exposure and Risk Assessment 33(4): 631-638.
Ledesma, E., Rendueles, M. and Díaz, M., 2016. Contamination of meat products during smoking by polycyclic aromatic hydrocarbons: processes and prevention. Food Control 60: 64-87.
Mandel, J., 1964. The statistical analysis of experimental data. Wiley & Sons, New York, NY, USA.
Naccari, C., Cristani, M., Giofrè, F., Ferrante, M., Siracusa, L. and Trombetta, D., 2011. PAHs concentration in heat-treated milk Samples. Food Research International 44(3): 716-724.
Shi, L., Zhang, D. and Liu, Y., 2016. Survey of polycyclic aromatic hydrocarbons of vegetable oils and oilseeds by GC-MS in China. Food Additives & Contaminants: Part A 33(4): 603-611.
Tfouni, S.A.V., Padovani, G.R., Reis, R.M., Furlani, R.P.Z. and Camargo, M.C.R., 2014. Incidence of polycyclic aromatic hydrocarbons in vegetable oil blends. Food Control 46: 539-543.
Visioli, F. and Strata, A., 2014. Milk, dairy products, and their functional effects in humans: a narrative review of recent evidence. Advances in Nutrition 5(2): 131-143.
Wen, Y.Q., Liu, Y.L., Xu, L.L., Yu, W.X. and Ma, Y.X., 2017. Occurrence of polycyclic aromatic hydrocarbons in various types of raw oilseeds from different regions of China. Food Add?t?ves & Contam?nants: Part B 10(4): 275-283.
Zelinkova, Z. and Wenzl, T., 2015. The Occurrence of 16 EPA PAHs in food – a review. Polycyclic Aromatic Compounds: 1-37.