Main Article Content
egg, ELISA test kit, EU MoniQA project, food allergens, milk, reference material, validation
Background Effective allergenic risk assessment and management are important to limit the use of precautionary statements such as ‘may contain’ and to be able to protect allergic consumers. However, such approaches require reliable analytical tools for the detection of allergens in food. Very few validation data are available for the comparison of results obtained with different allergen detection methods. This is certainly due to the lack of harmonized validation protocols and of recognized reference materials. Aims The Monitoring and Quality Assurance Working Group on Food Allergens will provide incurred reference materials with egg and milk proteins at various concentrations. Materials and Methods The development of an incurred reference material for the analysis of milk and egg allergens in a baked cookie food matrix is described. Results and Discussion We present the results of the development of the incurred reference material and a prering trial with two incurred reference materials for milk detection methods: cookies and soy-based infant formula. Conclusions The material produced seems to be suitable as reference material as well as for testing the performance of test kits. The forthcoming validation study according to the harmonized validation protocol will significantly and positively impact on future validation procedures.
International Standards Organisation. (2003) ISO 17511. ISO, Geneva.
Kerbach S., Alldrick A.J., Crevel R.W.R., D ?om ?ot ?or L., DunnGalvinA., Mills E.N.C., Pfaff S., Poms R.E., Popping B., T ?om ?osk ?ozi S.(2009) Managing food allergens in the food supply chain –viewed from different stakeholders perspectives. Quality Assurance and Safety of Crops & Foods,1, 50–60.
Poms R.E., Agazzi M.E., Bau A., Brohee M., Capelletti C., Norgaard J.V., Anklam E. (2005) Inter-laboratory validation study of five commercial ELISA test kits for the determination of peanut proteins in biscuits and dark chocolate. Food Additives and Contaminants, 22, 104–112.
Poms R.E., Anklam E., Emons H. (2006) Reference materials and method validation. In: Detecting Food Allergens eds Hefle S., Koppelman S. pp. 348–356, Woodhead Publishing Ltd., Cambridge, UK.
Poms R.E., Capelletti C., Anklam E. (2004a) Effect of roasting history and buffer composition on peanut protein extraction efficiency. Molecular Nutrition and Food Research, 48, 459–464.
Poms R.E., Klein C.L., Anklam E. (2004b) Methods for allergen analysis in food: a review. Food Additives and Contaminants, 21, 1–31.
Poms R.E., Thomas M., Finglas P., Astley S., Spichtinger D., RoseM., Popping B., Alldrick A., Egmond H.v., Solfrizzo M., MillsC.E.N., Kneifel W., Paulin S., Oreopoulou V., To K.A., CarceaM., Tureskja H., Saarela M., Haugen J.-E., Gross M. (2009) MoniQA (Monitoring and Quality Assurance) – An EU-funded Network of Excellence (NoE) working towards the harmonisation of worldwide food quality and safety monitoring and control strategies. Quality Assurance and Safety of Crops & Foods,1, 9–22.
Scaravelli E., Broh ?ee M., Marchelli R., Van Hengel A.J. (2008) Development of three real-time PCR assays to detect peanut allergen residue in processed food products. European Food Research and Technology,227,857–869.
Taylor S.L., Nordlee J.A., Niemann L.M., Lambrecht D.M. (2009) Allergen immunoassays–considerations for use of naturally incurred standards. Analytical and Bioanalytical Chemistry, 395, 83–92.