Physicochemical properties of serish root (Eremurus spectabilis) fructan as affected by drying methods

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A. Pourfarzad
M.B. Habibi Najafi
M.H. Haddad Khodaparast
M. Hassanzadeh Khayyat


freeze drying, fructan, oven drying, serish root, spray drying, vacuum drying


The fructans, inulin and oligofructose, are relatively unique functional food components because of their chemical nature and the combination of their physiological and nutritional effects that affect gastrointestinal functions. In the present study, the physicochemical properties of dried serish root fructans prepared by application of freeze drying, spray drying, vacuum drying and oven drying at 80 and 105 °C were evaluated. The oven-dried and freeze-dried fructans seem to be more hygroscopic than the spray and vacuum oven-dried fructans. The oven-dried sample at 105 °C showed the least water solubility among all samples. Fructan contents, purities, and the degree of polymerisation for dried serish fructan samples were 85-87, 79-80 and 13-14%, respectively. The drying method affected the microstructure of the fructan particles leading to differences in their physical properties (particle size, shape, zeta potential, colour and solubility). The colour analysis suggested that the successive combination of spray drying and freeze drying methods had the least negative effect on appearance among the drying treatments. As evidenced by the X-ray diffractograms, the oven and spray-dried samples exhibited amorphous structures, while the freeze and vacuum drying of serish fructans tend to produce semi-crystalline dried products. Differential scanning calorimetry showed that Freeze-drying gave the highest onset and mid-point melting temperatures, whereas oven and spray drying showed the lower melting enthalpy than the freeze dried and vacuum dried samples. FT-IR spectral analysis showed no major structural differences in fructan samples produced by various drying treatments.

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Acedo-Carrillo, J., Rosas-Durazo, A., Herrera-Urbina, R., Rinaudo, M., Goycoolea, F. and Valdez, M., 2006. Zeta potential and drop growth of oil in water emulsions stabilized with mesquite gum. Carbohydrate Polymers 65: 327-336.
Ahlneck, C. and Zografi, G., 1990. The molecular basis of moisture effects on the physical and chemical stability of drugs in the solid state. International Journal of Pharmaceutics 62: 87-95.
Association of Official Analytical Chemists (AOAC), 1995. Official Methods of Analysis of AOAC. AOAC, Rockville, MD, USA.
Barbosa-Canovas, G., Juliano, P. and Peleg, M., 2006. Engineering properties of foods. Encyclopedia of Life Support Systems (EOLSS): 1-32.
Blecker, C., Chevalier, J.P., Van Herck, J.C., Fougnies, C., Deroanne, C. and Paquot, M., 2001. Inulin: its physicochemical properties and technological functionality. Recent Research Developments in Agricultural & Food Chemistry 5: 125-131.
Botrel, D.A., Borges, S.V., Fernandes, R.V.D.B. and Do Carmo, E.L., 2014. Optimization of fish oil spray drying using a protein: inulin system. Drying Technology 32: 279-290.
Brayan, C., 1989. Bulbs. Timber Press, Portland, OR, USA.
Brickell, C., 1996. Encyclopedia of garden plants. Dorling Kindersley Limited, London, UK.
Cakié, M., Nikolié, G. and Ilie, L., 2002. FTIR spectra of iron (III) complexes with dextran, pullulan and inulin oligomers. Bulletin of the Chemists and Technologists of Macedonia 21: 135-146.
Cano-Chauca, M., Stringheta, P., Ramos, A. and Cal-Vidal, J., 2005. Effect of the carriers on the microstructure of mango powder obtained by spray drying and its functional characterization. Innovative Food Science & Emerging Technologies 6: 420-428.
Caparino, O., Tang, J., Nindo, C., Sablani, S., Powers, J. and Fellman, J., 2012. Effect of drying methods on the physical properties and microstructures of mango (Philippine ‘Carabao’ var.) powder. Journal of Food Engineering 111: 135-148.
Chiou, D., Langrish, T. and Braham, R., 2008. The effect of temperature on the crystallinity of lactose powders produced by spray drying. Journal of Food Engineering 86: 288-293.
Cortes-Rojas, D.F. and Oliveira, W.P., 2012. Physicochemical properties of phytopharmaceutical preparations as affected by drying methods and carriers. Drying Technology 30: 921-934.
Crockett, J.U., 1972. The time-life encyclopedia of gardening, 1. Time-Life Books, New York, NY, USA.
Dashti, M., Tavakoli, H., Zarif Ketabi, H. and Paryab, A., 2005. Ecological requirements of plant of Eremurus (Eremurus spectabilisM.B.) in Khorasan province [in Persian]. Iranian Journal of Range and Desert Research 12: 153-165.
Dogan, M. and Toker, O., 2015. Hydroxymethylfurfural content and physicochemical properties of the caramel samples enriched with different dietary fibres. Quality Assurance and Safety of Crops & Foods 7: 277-285.
Grube, M., Bekers, M., Upite, D. and Kaminska, E., 2002. Infrared spectra of some fructans. Journal of Spectroscopy 16: 289-296.
Hansen, O. and Madsen, R., 1992. Method for preparing a mixture of saccharides. US Patent number 5,254,174.
Kim, G.J., Bang, K.H., Kim, Y.B. and Rhee, Y.H., 2000. Preparation and characterization of native poly (3-hydroxybutyrate) microspheres from Ralstonia eutropha. Biotechnology Letters 22: 1487-1492.
Lingyun, W., Jianhua, W., Xiaodong, Z. and Yalin, F., 2007. Studies on the extracting technical conditions of inulin from Jerusalem artichoke tubers. Journal of Food Engineering 79: 1087-1093.
Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 420-428.
Moghari, A.A., Razavi, S., Ehsani, M., Mousavi, M. and Nia, T.H., 2015. Chemical, proteolysis and sensory attributes, and probiotic microorganisms viability of Iranian ultrafiltered-Feta cheese as a function of inulin concentration and storage temperature. Quality Assurance and Safety of Crops & Foods 7: 217-224.
Monsoor, M.A., 2005. Effect of drying methods on the functional properties of soy hull pectin. Carbohydrate Polymers 61: 362-367.
Muir, J.G., Shepherd, S.J., Rosella, O., Rose, R., Barrett, J.S. and Gibson, P.R., 2007. Fructan and free fructose content of common Australian vegetables and fruit. Journal of Agricultural and Food Chemistry 55: 6619-6627.
Nikolic, G.S. and Cakic, M.D., 2007. Physical investigation of the colloidal iron-inulin complex. Colloid journal 69: 464-473.
O’Brien, C., Mueller, A., Scannell, A. and Arendt, E., 2003. Evaluation of the effects of fat replacers on the quality of wheat bread. Journal of Food Engineering 56: 265-267.
Oliveira, W., Freitas, L., Freire, J., Silveira, A. and Ferreira, M., 2012. Drying of pharmaceutical products. In: Freire, J.T., Silveira, A.M. and Ferreira, M.C. (eds.) Transport phenomena in particulate systems. Bentham Science, Oak Park, IL, USA, pp. 148-171.
Panchev, I., Delchev, N., Kovacheva, D. and Slavov, A., 2011. Physicochemical characteristics of inulins obtained from Jerusalem artichoke (Helianthus tuberosus L.). European Food Research and Technology 233: 889-896.
Paseephol, T., Small, D. and Sherkat, F., 2007. Process optimisation for fractionating Jerusalem artichoke fructans with ethanol using response surface methodology. Food Chemistry 104: 73-80.
Pourfarzad, A., Habibi Najafi, M., Haddad Khodaparast, M. and Hassanzadeh Khayyat, M., 2015. Characterization of fructan extracted from Eremurus spectabilis tubers: a comparative study on different technical conditions. Journal of Food Science and Technology 52: 2657-2667.
Pourfarzad, A., Habibi Najafi, M.B., Haddad Khodaparast, M.H., Hassanzadeh Khayyat, M. and Malekpour, A., 2014. Fractionation of Eremurus spectabilis fructans by ethanol: Box-Behnken design and principal component analysis. Carbohydrate Polymers 106: 374-383.
Prosky, L. and Hoebregs, H., 1999. Methods to determine food inulin and oligofructose. The Journal of Nutrition 129: 1418-1423.
Ratti, C., 2001. Hot air and freeze-drying of high-value foods: a review. Journal of Food Engineering 49: 311-319.
Roberfroid, M. and Delzenne, N., 1998. Dietary fructans. Annual Review of Nutrition 18: 117-143.
Roberfroid, M.B., 2005. Introducing inulin-type fructans. British Journal of Nutrition 93: 13-26.
Ronkart, S.N., Deroanne, C., Paquot, M., Fougnies, C., Lambrechts, J.C. and Blecker, C.S., 2007. Characterization of the physical state of spray-dried inulin. Food Biophysics 2: 83-92.
Rowlands, W.N. and O’Brien, R.W., 1995. The dynamic mobility and dielectric response of kaolinite particles. Journal of Colloid and Interface Science 175: 190-200.
Shene, C., Cabezas, M. and Bravo, S., 2003. Effect of drying air temperature on drying kinetics parameters and fructan content in Helianthus tuberosus and Cichorium intybus. Drying Technology 21: 945-956.
Sherman, P., 1970. Rheology of dispersed systems, industrial rheology. Academic Press Inc., London, UK, pp. 97-183.
Shi, A.M., Wang, L.J., Li, D. and Adhikari, B., 2012. The effect of annealing and cryoprotectants on the properties of vacuum-freeze dried starch nanoparticles. Carbohydrate Polymers 88: 1334-1341.
Souza, A.S., Borges, S.V., Magalhães, N.F., Ricardo, H.V., Cereda, M.P. and Daiuto, E.R., 2009. Influence of spray drying conditions on the physical properties of dried pulp tomato. Ciência e Tecnologia de Alimentos 29: 291-294.
Tang, X. and Pikal, M., 2004. Design of freeze-drying processes for pharmaceuticals: practical advice. Pharmaceutical Research 21: 191-200.
Tant, M. and Wilkes, G., 1981. An overview of the nonequilibrium behavior of polymer glasses. Polymer Engineering & Science 21: 874-895.
Toneli, J., Park, K., Negreiros, A. and Murr, F., 2010. Spray-drying process optimization of chicory root inulin. Drying Technology 28: 369-379.
Tonon, R.V., Brabet, C. and Hubinger, M.D., 2008. Influence of process conditions on the physicochemical properties of açai (Euterpe oleraceae Mart.) powder produced by spray drying. Journal of Food Engineering 88: 411-418.
Vendruscolo, C.W., Ferrero, C., Pineda, E.A., Silveira, J.L., Freitas, R.A., Jiménez-Castellanos, M.R. and Bresolin, T., 2009. Physicochemical and mechanical characterization of galactomannan from Mimosa scabrella: effect of drying method. Carbohydrate Polymers 76: 86-93.
Wang, C. and Johnson, L., 2001. Functional properties of hydrothermally cooked soy protein products. Journal of the American Oil Chemists’ Society 78: 189-195.
Wang, Y., Li, D., Wang, L.-J., Li, S.-J. and Adhikari, B., 2010. Effects of drying methods on the functional properties of flaxseed gum powders. Carbohydrate Polymers 81: 128-133.
Zhao, Q., Kennedy, J.F., Wang, X., Yuan, X., Zhao, B., Peng, Y. and Huang, Y., 2011. Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology. International journal of biological macromolecules 49: 181-187.
Zhou, Y.G., Li, D., Wang, L.J., Li, Y., Yang, B.-N., Bhandari, B., Chen, X.D. and Mao, Z.H., 2009. Effect of water content on thermal behaviors of common buckwheat flour and starch. Journal of Food Engineering 93: 242-248.