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antimicrobial activity; Dolichos lablab; lablab bean; rice noodles; shelf-life
This study provides an application of new, natural source of crude peptide extract from Lablab bean (CPL). Use of additive such as benzoate in the rice noodle industry is a common practice and has several bene?cial effects on quality and shelf-life. However, the shelf-life of semi-dried rice noodles can be extended by crude peptide extract with acceptable quality when compared to other additives. This research aimed to extract CPL and determine its effect on the growth of microorganisms. The use of the CPL to extend the shelf-life of semi-dried rice noo-dles was compared with other natural extracts (chitosan and thymol) and benzoic acid. The CPL samples were extracted using 5% pepsin and incubated for up to 24 h. CPL at 200 mg/mL could be used as the growth inhibitor for Bacillus cereus but not for Staphylococcus aureus and Escherichia coli. It was found that noodles with CPL had the highest cooking loss (4.69) and lowest tensile strength (22.6 g.force). Overall-liking scores showed slightly liked (6.0 out of 9) and 68% of the consumers accepted the CPL-treated noodles. Shelf-life testing showed that CPL could extend the shelf-life of the noodles for 3 days than the control (1 day). Using 200 mg/mL of CPL could extend the shelf-life more than 3 times when compared to the control noodle (no additive). The CPL was nearly as effective as benzoic acid that could be kept for 3 days. Hedonic score in overall-liking showed a slightly like (6.2) for the noodles with CPL. This study suggests the application of adding CPL could be used as new natural additive and seems to be promising to apply in many food products such as pasta or sausages.
AOAC, 2007. Official methods of analysis of AOAC International. 18th ed. Methods 950.46, 976.06, 960.39, 942.05, 962.09, 994.12, 999.11, and 997.02. AOAC International, Gaithersburg, MD.
Arciola, C.R., Campoccia, D., Ravaioli, S. and Montanaro, L., 2019. Polysaccharide intercellular adhesion in bio?lm: structural and regulatory aspects. Frontiers in Cellular and Infection Microbiology 5(7): 1?10. http://dx.doi.org/10.3389/ fcimb.2015.00007
Berthold-Pluta, A., Pluta, A., Garbowska, M. and Stefanska, I., 2019. Prevalence and toxicity characterization of Bacillus cereus in food products from Poland. Foods 8(7): 269?280. http://dx.doi. org/10.3390/foods8070269
Borijindakul, L. and Phimolsiripol, Y., 2013. Physicochemical and functional properties of starch and germinated flours from Dolichos lablab. Food and Applied Bioscience Journal 1: 69?80. Retrieved from https://li01.tci-thaijo.org/index.php/fabjournal/ article/view/77360
Cantor, S., Vargas, L., Rojas-A, O.E., Yarce, C.J., Salamanca, C.H. and Onate-Garzon, J., 2019. Evaluation of the antimicrobial activity of cationic peptides loaded in surface-modifed nanoliposomes against foodborne bacteria. International Journal of Molecular Science 20(3): 1?15. http://dx.doi.org/10.3390/ijms20030680
Cheison, S.C., Bor, E.K., Faraj, A.K. and Kulozik, U., 2012. Selective hydrolysis of alpha-lactalbumin by acid protease A of?fers potential for beta-lactoglobulin purifcation in whey proteins. LWT-Food Science Technology 49(1): 117?122. http://dx.doi.org/10.1016/j. lwt.2012.03.022
Cham, S. and Suwannaporn, P., 2010. Efffect of hydrothermal treatment of rice flour on various rice noodles quality. Journal of Cereal Science 51(3): 284?291. http://dx.doi.org/10.1016/j.jcs.2010.01.002
Chung, H.J., Cho, A. and Lim, S.T., 2012. Efffect of heat-moisture treatment for utilization of germinated brown rice in wheat noodle. LWT-Food Science Technology 47(2): 342?347. http:// dx.doi.org/10.1016/j.lwt.2012.01.029
Codex, 2009. General standard for food additives codex STAN 192-1995. Food and Agriculture Organization of the United Nation, World Health Organization. Available at: http://www.fao.org/ gsfaonline/docs/CXS_192e.pdf
Dia, V.P., Bringe, N.A. and Mejia, E.G., 2014. Peptides in pepsin– pancreatin hydrolysates from commercially available soy products that inhibit lipopolysaccharide-induced inflammation in macrophages. Food Chemistry 152(1): 423-431. http://dx.doi. org/10.1016/j.foodchem.2013.11.155
Duangmal, K. and Saetongtae, B., 2014. Protein modifcation using proteases and its applications. KKU Science Journal 42: 274?288.
El-Araby, M.M., El-Shatoury, E.H., Soliman, M.M. and Shaaban, H.F., 2020. Characterization and antimicrobial activity of lectins purifed from three Egyptian leguminous seeds. AMB Express 10: 90. http://dx.doi.org/10.1186/s13568-020-01024-4
Gasu, E.N., Ahor, H.S. and Borquaye, L.S., 2018. Peptide extract from Olivancillaria hiatula exhibits broad-spectrum antibacterial activity. BioMed Research International 2018: 1-11. http:// dx.doi.org/10.1155/2018/6010572
Guinane, C.M., Kent, R.M., Norberg, S., O’Connor, P.M., Cotter, P.D., Hill, C., et al. 2015. Generation of the antimicrobial peptide caseicin A from casein by hydrolysis with thermolysin enzymes. International Dairy Journal 49: 1-7. http://dx.doi. org/10.1016/j.idairyj.2015.04.001
Hossain, S., Ahmed, R., Bhowmick, S., Mamun, A.A. and Hashimoto, M., 2016. Proximate composition and fatty acid analysis of Lablab purpureus (L.) legume seed; implicates to both protein and essential fatty acid supplementation. SpringerPlus 5: 1-10. http://dx.doi.org/10.1186/s40064-016-3587-1
Inetianbor, J.E., Yakubu, J.M. and Ezeonu, S.C., 2015. Efffects of food additives and preservatives on man- A review. Asian Journal of Science and Technology 6(2): 1118-1135.
Intorasoot, S., 2013. Antimicrobial peptides: the natural proteins and the future application for treatment of infectious diseases. Bulletin Chiang Mai Associated Medical Sciences 46(1): 1-19.
Jaisankar, P. and Manivannan, K., 2018. Ef?fect of dif?ferent levels of nitrogen and phosphorus on growth and yield characters of bush bean (Dolichos lablab var. typicus). Plant Archives 18(2): 2194-2198. Available at: http://www.plantarchives.org/18-02/2194-2198%20(4275).pdf
Kala, B.K., Soris, P.T., Mohan, V.R. and Vadivel, V., 2010. Nutrient and chemical evaluation of raw seed of five varieties of Lablab purpureus (L.) sweet. Advances in Bioresearch 1(1): 44-53. Available at: http://www.soeagra.com/abr/vol1/44-53.pdf
Kuipers, A., Stapels, D.A.C., Weerwind, L.T., Ko, Y.P., Ruyken, M., Lee, J.C., et al. 2016. The Staphylococcus aureus polysaccharide capsule and Efb-dependent brinogen shield act in concert to protect against phagocytosis. Microbiology 162(7): 1185?1194. http://dx.doi.org/10.1099/mic.0.000293
Laokuldilok, T., Potivas, T., Kanha, N., Surawang, S., Seesuriyachan, P., Wangtueai, S., et al. 2017. Physicochemical, antioxidant, and antimicrobial properties of chitooligosaccharides produced using three diffferent enzyme treatments. Food Bioscience 18: 28–33. http://dx.doi.org/10.1016/j.fbio.2017.03.004
Lei, J., Sun, L., Huang, S., Zhu, C., Li, P., He, J., et al. 2019. The anti-microbial peptides and their potential clinical applications. American Journal of Translational Research 11(7): 3919-3931. Available at: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC6684887/.
Li, M., Zhu, K.X., Guo, X.N., Brijs, K. and Zhou, H.M., 2014. Natural additives in wheat-based pasta and noodle products: opportunities for enhanced nutritional and functional properties. Comprehensive Reviews in Food Science and Food Safety 13(4): 347?357. http://dx.doi.org/10.1111/1541-4337.12066
Liu, Y.F., Chaiwanichsiri, S. and Laohasongkram, K., 2014. Physicochemical properties of flour recovered from broken rice noodles during production. International Journal of Food Science and Technology 49(7): 1722?1728. http://dx.doi. org/10.1111/ijfs.12481
Ministry of Public Health, 2016.
Notifcation of the Ministry of Public Health (No. 381) B.E 2559 (2016) Re: Food Additives (No.4). Bangkok, Thailand. Available at: http://www.fda.moph. go.th/sites/food/FoodAdditives/MOPH(No.381)B.E.2559(2016) Re_AdditiveNo.4.pdf.
Mirzapour-Kouhdasht, A., Moosavi-Nasab, M., Kim, Y.-M. and Eun, J.-B., 2020. Antioxidant mechanism, antibacterial activity, and functional characterization of peptide fractions obtained from barred mackerel gelatin with a focus on application in carbonated beverages. Food Chemistry 342: 128339. http://dx.doi. org/10.1016/j.foodchem.2020.128339
Patel, H., Vaghasiya, Y., Vyas, B.R.M. and Chanda, S., 2012. Antibiotic-resistant Staphylococcus aureus: a challenge to researchers and clinicians. Bacteriology Journal 2(2): 23?45. http://doi.org/10.3923/bj.2012.23.45
Phimolsiripol, Y., Siripatrawan, U., Teekachunhatean, S., Wangtueai, S., Seesuriyachan, P., Surawang, S., et al. 2017. Technological properties, in vitro starch digestibility and in vivo glycaemic index of bread containing crude malva nut gum. International Journal of Food Science and Technology 52(4): 1035-1041. http://dx.doi.org/10.1111/ijfs.13369
Qazi, I.M., Rakshit, S.K., Tran, T., Javidullah and Khan, M.Z., 2014. Efffect of blending selected tropical starches with rice flour on the cooking quality and texture of rice based noodles. Sarhad Journal of Agriculture 30(2): 257?263.
Rahman, S.A. and Akhter, S.M., 2018. Antibacterial and cytotoxic activity of seeds of white hyacinth bean (Lablab purpureus L. sweet “white”). Journal of Advanced Biotechnology and Experimental Therapeutics 1(2): 49–54. http://doi.org/10.5455/jabet.2018.d9
Ramos, Y., Gonzalez, A., Sosa-Acosta, P., Perez-Rivrsol, Y., Garcia, Y., Castellanos-Serra, L., et al. 2019. Sodium dodecyl sulfate free gel electrophoresis/electroelution sorting for peptide fractionation. Journal of Separation Science 42: 3712–3717. http://dx.doi.org/10.1002/jssc.201900495
Ratnayani, K., Panjaitan, I.W.S. and Puspawati, N.M., 2017. Screening potential antioxidant and antimicrobial activities of protein hydrolysates derived from germinated lablab bean, pigeon pea and kidney bean. Journal of Health Sciences and Medicine 1(1): 24–27. http://dx.doi.org/10.24843/JHSM.2017.v01.i01.p07
Reshmi, S.K, Aravinthan, K.M. and Devi, P.S., 2012. Antimicrobial activity of Basella alba fruit. International Journal of Pharmaceutical Sciences and Research 3(12): 4757?4761. http:// dx.doi.org/10.13040/IJPSR.0975-8232.3(12).4757-61
Saha, R.K., Tuhin, S.H.M., Jahan, N., Roy, A. and Roy, P., 2014. Antibacterial and antioxidant activities of a food lectin isolated from the seeds of Lablab purpureous. American Journal of Ethnomedicine 1(1): 8–17.
Saikhunthod, M. and Peerapattana, P., 2015. Wastes reduction in production process using green productivity: a case study in rice noodle factory. Journal of Applied Science 14(2): 40?57.
Sukamto, M., Sudiyono, S., Galih, P.D. and Karim, F., 2019. Ef?fects of pepsin on the separation of the globulin 7s and 11s fraction from Lablab bean (Komak) seeds (Dolichos lablab) and its functional properties. Pakistan Journal of Nutrition 18(8): 783?790. http:// dx.doi.org/10.3923/pjn.2019.783.790
Surin, S., Seesuriyachan, P., Thakeow, P., You, S.G. and Phimolsiripol, Y., 2018. Antioxidant and antimicrobial properties of polysaccharides from rice brans. Chiang Mai Journal of Science 45: 1372–1382.
Thomas, R., Yeoh, T.K., Wan-Nadiah, W.A. and Rajeev, B., 2014. Quality evaluation of flat rice noodles (Kway Teow) prepared from Bario and Basmati rice. Sains Malaysiana 43(3): 339?347.
Tiboonbun, W., Sungsri-in, M. and Moongngarm, A., 2011. Ef?fect of replacement of unripe banana flour for rice flour on physical properties and resistant starch content of rice noodle. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering 5: 558-5561. http://dx. doi.org/10.5281/zenodo.1083547
Tsutsumi, R. and Tsutsumi, Y.M., 2014. Peptides and proteins in whey and their bene?ts for human health. Austin Journal of Nutrition and Food Science 1(1): 1-9.
Vipra, A., Desai, N.S., Junjappa, P.R., Roy, P., Poonacha, N., Ravinder, P., et al. 2013. Determining the minimum inhibitory concentration of bacteriophages: Potential advantages. Advances in Microbiology 3(2): 181-190. http://dx.doi. org/10.4236/aim.2013.32028
Wandee, Y., Uttapap, D., Puncha-arnon, S., Puttanlek, C., Rungsardthong, V. and Wetprasit, N., 2014. Enrichment of rice noodles with fibre-rich fractions derived from cassava pulp and pomelo peel. International Journal of Food Science and Technology 49(1): 2348?2355. http://dx.doi.org/10.1111/ijfs.12554
Wang, X., Ma, Z., Li, X., Liu, L., Yin, X., Zhang, K., et al. 2018. Food additives and technologies used in Chinese traditional staple foods. Chemical and Biological Technology in Agriculture 5: 1. http://dx.doi.org/10.1186/s40538-017-0113-9
Wang, J. and Vermerris, W., 2016. Antimicrobial nanomaterials derived from natural products. Materials 9(4): 1-19. http://dx. doi.org/10.3390/ma9040255
Wang, J., Zhao, M., Zhao, Q. and Jiang, Y., 2007. Antioxidant properties of papain hydrolysates of wheat gluten in different oxidation systems. Food Chemistry 101(4): 1658?1663. http://dx.doi. org/10.1016/j.foodchem.2006.04.024
Wangtueai, S., Phimolsiripol, Y., Vichasilp, C., Regenstein, J.M. and Schoenlechner, R., 2020. Optimization of gluten-free functional noodles formulation enriched with fish gelatin hydrolysates. LWT- Food Science and Technology 133: 109977. http://dx.doi. org/10.1016/j.lwt.2020.109977
Wong, J.H. and Ng, T.B., 2005. Lunatusin, a trysin-stable antimicrobial peptide from Lima beans (Phaseolus lunatus L.). Peptides 26(11): 2086?2092. http://dx.doi.org/10.1016/j. peptides.2005.03.004
Zhang, X.P., Li, X.J., Yang, M.J., Yang, X. and Zhao, X., 2020. Ef?fect of antioxidant extracted from bamboo leaves on the quality of box-packaged sturgeon fillets stored at 4°C. Quality Assurance and Safety of Crops and Foods 12(2): 73–80. http://dx.doi. org/10.15586/qas.v12i2.690
Zhang, Z., Feng, L., Xu, H., Liu, C., Shah, N.P. and Wei, H., 2016. Detection of viable enterotoxin-producing Bacillus cereus and analysis of toxigenicity from ready-to-eat foods and infant formula milk powder by multiplex PCR. Journal of Dairy Science 99(2): 1047?1055. http://doi.org/10.3168/jds.2015