Design and application of soy protein isolate films reinforced with Janus nanoparticles for active packaging of minced meat
Main Article Content
Keywords
Janus nanoparticles; Soy protein isolate; Active packaging; Minced meat
Abstract
This study aimed to develop active soy protein isolate (SPI)-based packaging films incorporating Janus nanoparticles (JNPs) to improve the shelf life of minced meat. JNPs were synthesized using carboxymethyl cellulose and beeswax-based hydrophobic carbon dots and incorporated into SPI films at 0.03, 0.05, and 0.1% (w/w). Films were characterized for microstructure, antibacterial and antioxidant activities, mechanical properties, color, release pattern, and UV-blocking properties. FTIR and FESEM confirmed the homogeneous dispersion of JNPs. SPI-JNP0.1% films exhibited improved tensile strength (15.2 MPa vs. 9.6 MPa in SPI film) and elastic modulus (175.7 MPa vs. 112.5 MPa in SPI film), with slightly reduced elongation (31.4% vs. 36.5% in SPI film). Release studies showed that JNPs diffusion into food simulants was concentration-dependent. Antibacterial tests revealed superior inhibition of Listeria monocytogenes. When applied to beef, SPI-JNP films (0.1%) reduced total mesophilic and psychrotrophic counts by 4.4 and 4.2 log₁₀ CFU/g after 9 days at 7 °C. These results highlight the potential of JNPs as functional nanofillers in biodegradable food packaging.
References
Adilah, Z. A. M., Jamilah, B., & Hanani, Z. A. N. (2018). Functional and antioxidant properties of protein-based films incorporated with mango kernel extract for active packaging. Food Hydrocolloids, 74, 207–218. https://doi.org/10.1016/j.foodhyd.2017.08.017
Ahmed, M. W., Haque, M. A., Mohibbullah, M., Khan, M. S. I., Islam, M. A., Mondal, M. H. T., & Ahmmed, R. (2022). A review on active packaging for quality and safety of foods: Current trends, applications, prospects and challenges. Food Packaging and Shelf Life, 33, 100913. https://doi.org/10.1016/j.fpsl.2022.100913
Akrami, S., Saki, M., Marashi Hossaeini, S. M., Sabahi, S., & Noori, S. M. A. (2023). Application of soy protein-based films and coatings on the shelf life of food products: A mini-review of recent publications with emphasis on nanotechnology. Journal of Food Measurement and Characterization, 17(2), 1393–1401. https://doi.org/10.1007/s11694-022-01708-4
Alizadeh, N., Moradi, M., Molaei, R., & Razavi, R. (2026). Bacterial nanocellulose films functionalized with Janus nanoparticles: Preparation and application in chicken meat preservation and safety. Scientific Reports, 7, 1–56. https://doi.org/10.1038/s41598-026-39029-x
Amiri, E., Aminzare, M., Azar, H. H., & Mehrasbi, M. R. (2019). Combined antioxidant and sensory effects of corn starch films with nanoemulsion of Zataria multiflora essential oil fortified with cinnamaldehyde on fresh ground beef patties. Meat Science, 153, 66–74. https://doi.org/10.1016/j.meatsci.2019.03.004
Amjadi, S., Nazari, M., Alizadeh, S. A., & Hamishehkar, H. (2020). Multifunctional betanin nanoliposomes-incorporated gelatin/chitosan nanofiber/ZnO nanoparticles nanocomposite film for fresh beef preservation. Meat Science, 167, 108161. https://doi.org/10.1016/j.meatsci.2020.108161
ASTM. (2012). Designation D882-12: Standard test method for tensile properties of thin plastic sheeting. Annual Book of ASTM Standards. American Society for Testing and Materials, Philadelphia.
Collins, T., & Margesin, R. (2019). Psychrophilic lifestyles: Mechanisms of adaptation and biotechnological tools. Applied Microbiology and Biotechnology, 103(7), 2857–2871. https://doi.org/10.1007/s00253-019-09659-5
Divsalar, E., Moradi, M., Tajik, H., Molaei, R., Conte, A., & Del Nobile, M. A. (2025). Improving the shelf life of fresh pasta filata cheese using lactobacilli postbiotics and carbon dots. Quality Assurance and Safety of Crops and Foods, 17(2), 106–123. https://doi.org/10.15586/qas.v17i2.1530
Esmaeili Koutamehr, M., Moradi, M., Tajik, H., Molaei, R., Khakbaz Heshmati, M., & Alizadeh, A. (2023). Sour whey-derived carbon dots: Synthesis, characterization, antioxidant activity, and antimicrobial performance against foodborne pathogens. LWT - Food Science and Technology, 184, 114978. https://doi.org/10.1016/j.lwt.2023.114978
Ezati, P., & Rhim, J.-W. (2021). Fabrication of quercetin-loaded biopolymer films as functional packaging materials. ACS Applied Polymer Materials, 3(4), 2131–2137. https://doi.org/10.1021/acsapm.1c00177
Ezati, P., Rhim, J.-W., Molaei, R., Priyadarshi, R., Roy, S., Min, S., Kim, Y. H., Lee, S. G., & Han, S. (2022). Preparation and characterization of B-, S-, and N-doped glucose carbon dots: Antibacterial, antifungal, and antioxidant activity. Sustainable Materials and Technologies, 32, 397. https://doi.org/10.1016/j.susmat.2022.e00397
Ezati, P., Roy, S., & Rhim, J.-W. (2022). Pectin/gelatin-based bioactive composite films reinforced with sulfur-functionalized carbon dots. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 636, 128123. https://doi.org/10.1016/j.colsurfa.2021.128123
Fan, S., Yang, Q., Zhu, C., Li, X., Richel, A., Fauconnier, M., Fang, F., Zhang, D., & Hou, C. (2025). Zein/chitosan Janus film incorporated with tannic acid and cinnamon essential oil co-loaded Pickering emulsion for sustained controlled release and pork preservation. International Journal of Biological Macromolecules, 286, 138429. https://doi.org/10.1016/j.ijbiomac.2024.138429
Fu, B., Liu, Q., Liu, M., Chen, X., Lin, H., Zheng, Z., Zhu, J., Dai, C., Dong, X., & Yang, D.-P. (2022). Carbon dots enhanced gelatin/chitosan bio-nanocomposite packaging film for perishable foods. Chinese Chemical Letters, 33(10), 4577–4582. https://doi.org/10.1016/j.cclet.2022.03.048
Ghorbani, M., Moradi, M., Tajik, H., Molaei, R., & Alizadeh, A. (2024). Carbon dots embedded bacterial cellulose membrane as active packaging: Toxicity, in vitro release, and application in minced beef packaging. Food Chemistry, 433, 137311. https://doi.org/10.1016/j.foodchem.2023.137311
Ghorbani, M., Tajik, H., Moradi, M., Molaei, R., & Alizadeh, A. (2022). One-pot microbial approach to synthesize carbon dots from baker’s yeast-derived compounds for the preparation of antimicrobial membrane. Journal of Environmental Chemical Engineering, 10(3), 107525. https://doi.org/10.1016/j.jece.2022.107525
Guerrero, P., Garrido, T., Leceta, I., & de la Caba, K. (2013). Films based on proteins and polysaccharides: Preparation and physical–chemical characterization. European Polymer Journal, 49(11), 3713–3721. https://doi.org/10.1016/j.eurpolymj.2013.08.014
Hadavifar, S., Abedi-Firoozjah, R., Bahramian, B., Jafari, N., Sadeghi, S. M., Majnouni, S., Ebrahimi, B., Ehsani, A., & Tavassoli, M. (2025). Multifunctional performance of chitosan/soy protein isolation-based films impregnated carbon dots/anthocyanin derived from purple hull pistachio for tracking and extending the shelf life of fish. Food Hydrocolloids, 159, 110678. https://doi.org/10.1016/j.foodhyd.2024.110678
Hosseiniyeh, N., Mohtarami, F., Almasi, H., & Azizi, S. (2024). Soy protein isolate film activated by black seed oil nanoemulsion as a novel packaging for shelf-life extension of bulk bread. Food Science & Nutrition, 12(3), 1706–1723. https://doi.org/10.1002/fsn3.3864
Hu, J., Li, D., Huai, Q., Geng, M., Sun, Z., Wang, M., Wang, S., Li, Y., & Zheng, H. (2023). Development and evaluation of soybean protein isolate–based antibacterial nanocomposite films containing nano-TiO2. Industrial Crops and Products, 197, 116620. https://doi.org/10.1016/j.indcrop.2023.116620
Huang, X., Zhou, X., Dai, Q., & Qin, Z. (2021). Antibacterial, antioxidation, UV-blocking, and biodegradable soy protein isolate food packaging film with Mangosteen peel extract and ZnO nanoparticles. Nanomaterials, 11(12). https://doi.org/10.3390/nano11123337
Jia, R., Jiang, H., Jin, M., Wang, X., & Huang, J. (2015). Silver/chitosan-based Janus particles: Synthesis, characterization, and assessment of antimicrobial activity in vivo and vitro. Food Research International, 78, 433–441. https://doi.org/10.1016/j.foodres.2015.08.035
Konwar, A., Gogoi, N., Majumdar, G., & Chowdhury, D. (2015). Green chitosan–carbon dots nanocomposite hydrogel film with superior properties. Carbohydrate Polymers, 115, 238–245. https://doi.org/10.1016/j.carbpol.2014.08.021
Koshy, R. R., Reghunadhan, A., Mary, S. K., Sadanandan, S., Jose, S., Thomas, S., & Pothen, L. A. (2022). AgNP anchored carbon dots and chitin nanowhisker embedded soy protein isolate films with freshness preservation for active packaging. Food Packaging and Shelf Life, 33, 100876. https://doi.org/10.1016/j.fpsl.2022.100876
Kumar, L., & Gaikwad, K. K. (2023). Carbon dots for food packaging applications. Sustainable Food Technology, 1(2), 185–199. https://doi.org/10.1039/D2FB00020B
Li, W., Li, X., Wang, Q., Pan, Y., Wang, T., Wang, H., Song, R., & Deng, H. (2014). Antibacterial activity of nanofibrous mats coated with lysozyme-layered silicate composites via electrospraying. Carbohydrate Polymers, 99, 218–225. https://doi.org/10.1016/j.carbpol.2013.07.055
Lin, F., Wang, Z., & Wu, F.-G. (2022). Carbon dots for killing microorganisms: An update since 2019. Pharmaceuticals, 15(10). https://doi.org/10.3390/ph15101236
Liu, Z., McClements, D. J., Shi, A., Zhi, L., Tian, Y., Jiao, B., Liu, H., & Wang, Q. (2022). Janus particles: A review of their applications in food and medicine. Critical Reviews in Food Science and Nutrition, 6, 1–12. https://doi.org/10.1080/10408398.2022.2067831
Ma, Z., Xing, Z., Zhao, Y., Zhang, H., Xin, Q., Liu, J., Qin, M., Ding, C., & Li, J. (2023). Lotus leaf inspired sustainable and multifunctional Janus film for food packaging. Chemical Engineering Journal, 457, 141279. https://doi.org/10.1016/j.cej.2023.141279
Madannejad, R., Shoaie, N., Jahanpeyma, F., Darvishi, M. H., Azimzadeh, M., & Javadi, H. (2019). Toxicity of carbon-based nanomaterials: Reviewing recent reports in medical and biological systems. Chemico-Biological Interactions, 307, 206–222. https://doi.org/10.1016/j.cbi.2019.04.036
Meerasri, J., Sukatta, U., Rugthaworn, P., Klinsukhon, K., Khacharat, L., Sakayaroj, S., Chollakup, R., & Sothornvit, R. (2024). Synergistic effects of thyme and oregano essential oil combinations for enhanced functional properties of sericin/pectin film. International Journal of Biological Macromolecules, 263, 130288. https://doi.org/10.1016/j.ijbiomac.2024.130288
Mirmoeini, S. S., Moradi, M., Tajik, H., Almasi, H., & Gama, F. M. (2023). Cellulose/Salep-based intelligent aerogel with red grape anthocyanins: Preparation, characterization, and application in beef. Food Chemistry, 425, 136493. https://doi.org/10.1016/j.foodchem.2023.136493
Mirtalebi, S. S., Almasi, H., & Alizadeh Khaledabad, M. (2019). Physical, morphological, antimicrobial, and release properties of novel MgO-bacterial cellulose nanohybrids prepared by in-situ and ex-situ methods. International Journal of Biological Macromolecules, 128, 848–857. https://doi.org/10.1016/j.ijbiomac.2019.02.007
Molaei, R., Razavi, R., Omer, A. K., Javid, A. L., Ezati, P., Nikfarjam, N., Lim, L.-T., & Moradi, M. (2025). Hydrophobic carbon dots: An overview of the synthesis, purification, cytotoxicity, and potential applications in food safety and analytical chemistry. Quality Assurance and Safety of Crops & Foods, 17(2), 136–164. https://doi.org/10.15586/qas.v17i2.1532
Mostafa, H., Airouyuwaa, J. O., Hamed, F., Wang, Y., & Maqsood, S. (2023). Structural, mechanical, antioxidant, and antibacterial properties of soy protein isolate (SPI)-based edible food packaging films as influenced by nanocellulose (NC) and green extracted phenolic compounds from date palm leaves. Food Packaging and Shelf Life, 38, 101124. https://doi.org/10.1016/j.fpsl.2023.101124
Nikfarjam, N., Razavi, R., Moradi, M., & Molaei, R. (2025). Green synthesis of carbon dots from onion juice and ex-situ embedding for antimicrobial/ultraviolet protective nanocellulose films. Food Safety and Packaging, 1(1), 24–32. https://doi.org/10.30466/fsp.2025.56060.1006
Nwabor, O. F., Singh, S., Paosen, S., Vongkamjan, K., & Voravuthikunchai, S. P. (2020). Enhancement of food shelf life with polyvinyl alcohol-chitosan nanocomposite films from bioactive Eucalyptus leaf extracts. Food Bioscience, 36, 100609. https://doi.org/10.1016/j.fbio.2020.100609
Oliveira, A. V., da Silva, A. P. M., Barros, M. O., de Sá M. Souza Filho, M., Rosa, M. F., & Azeredo, H. M. C. (2018). Nanocomposite films from mango kernel or corn starch with starch nanocrystals. Starch - Stärke, 70(11–12), 1800028. https://doi.org/10.1002/star.201800028
Rani, P., Yu, X., Liu, H., Li, K., He, Y., Tian, H., & Kumar, R. (2021). Material, antibacterial, and anticancer properties of natural polyphenols incorporated soy protein isolate: A review. European Polymer Journal, 152, 110494. https://doi.org/10.1016/j.eurpolymj.2021.110494
Rani, S., Kumar, K. D., Mandal, S., & Kumar, R. (2020). Functionalized carbon dot nanoparticles reinforced soy protein isolate biopolymeric film. Journal of Polymer Research, 27(10), 1–10. https://doi.org/10.1007/s10965-020-02276-1
Razavi, R., Molaei, R., Moradi, M., Tajik, H., Ezati, P., & Yordshahi, A. S. (2020). Biosynthesis of metallic nanoparticles using mulberry fruit (Morus alba L.) extract for the preparation of antimicrobial nanocellulose film. Applied Nanoscience, 10(2), 465–476. https://doi.org/10.1007/s13204-019-01137-8
Razavi, R., Tajik, H., Molaei, R., McClements, D. J., & Moradi, M. (2024). Janus nanoparticles synthesized from hydrophobic carbon dots and carboxymethyl cellulose: Novel antimicrobial additives for fresh food applications. Food Bioscience, 62, 105171. https://doi.org/10.1016/j.fbio.2024.105171
Roila, R., Sordini, B., Esposto, S., Ranucci, D., Primavilla, S., Valiani, A., Taticchi, A., Branciari, R., & Servili, M. (2022). Effect of the application of a green preservative strategy on minced meat products: Antimicrobial efficacy of olive mill wastewater polyphenolic extract in improving beef burger shelf-life. Foods, 11(16). https://doi.org/10.3390/foods11162447
Roy, S., Ezati, P., & Rhim, J.-W. (2021). Gelatin/carrageenan-based functional films with carbon dots from enoki mushroom for active food packaging applications. ACS Applied Polymer Materials, 3(12), 6437–6445. https://doi.org/10.1021/acsapm.1c01175
Sattariazar, S., Arsalani, N., & Nejad Ebrahimi, S. (2023). Biological assessment of synthesized carbon dots from polyphenol enriched extract of pomegranate peel, incorporated with Mentha piperita essential oil. Materials Chemistry and Physics, 294, 126981. https://doi.org/10.1016/j.matchemphys.2022.126981
Sayadi, M., Mojaddar Langroodi, A., Amiri, S., & Radi, M. (2022). Effect of nanocomposite alginate-based film incorporated with cumin essential oil and TiO2 nanoparticles on chemical, microbial, and sensory properties of fresh meat/beef. Food Science & Nutrition, 10(5), 1401–1413. https://doi.org/10.1002/fsn3.2724
Semsari, E., Tajik, H., Molaei, R., & Moradi, M. (2024). Innovative aerosol technology using lemon peel-derived carbon dots for improving shelf life of beef. Applied Food Research, 4, 100499. https://doi.org/10.1016/j.afres.2024.100499
Shafipour Yordshahi, A., Moradi, M., Tajik, H., & Molaei, R. (2020). Design and preparation of antimicrobial meat wrapping nanopaper with bacterial cellulose and postbiotics of lactic acid bacteria. International Journal of Food Microbiology, 321, 108561. https://doi.org/10.1016/j.ijfoodmicro.2020.108561
Shang, W., Ye, M., Cai, T., Zhao, L., Zhang, Y., Liu, D., & Liu, S. (2018). Tuning of the hydrophilicity and hydrophobicity of nitrogen doped carbon dots: A facile approach towards high efficient lubricant nanoadditives. Journal of Molecular Liquids, 266, 65–74. https://doi.org/10.1016/j.molliq.2018.06.042
Sul, Y., Ezati, P., & Rhim, J.-W. (2023). Preparation of chitosan/gelatin-based functional films integrated with carbon dots from banana peel for active packaging application. International Journal of Biological Macromolecules, 246, 125600. https://doi.org/10.1016/j.ijbiomac.2023.125600
Tripathi, S., Kumar, L., Deshmukh, R. K., & Gaikwad, K. K. (2024). Ultraviolet blocking films for food packaging applications. Food and Bioprocess Technology, 17(6), 1563–1582. https://doi.org/10.1007/s11947-023-03221-y
Wang, Z., Chen, Y., Zhang, N., Zhang, R. X., He, R., Ju, X., & Mamadalieva, N. Z. (2023). Plant protein nanogel–based patchy Janus particles with tunable anisotropy for perishable food preservation. Food Frontiers, 4(2), 795–806. https://doi.org/10.1002/fft2.219
Wasilewska, A., Bielicka, M., Klekotka, U., & Kalska-Szostko, B. (2023). Nanoparticle applications in food – A review. Food & Function, 14(6), 2544–2567. https://doi.org/10.1039/D2FO02180C
Xue, J., Ma, C., Yang, S., Guo, S., Yin, X., Fan, J., Li, X., Wang, M., & Teng, G. (2024). Janus hydrogel loaded with a CO2-generating chemical reaction system: Construction, characterization, and application in fruit and vegetable preservation. Food Chemistry, 458, 140271. https://doi.org/10.1016/j.foodchem.2024.140271
Yang, J., Zhang, X., Ma, Y.-H., Gao, G., Chen, X., Jia, H.-R., Li, Y.-H., Chen, Z., & Wu, F.-G. (2016). Carbon dot-based platform for simultaneous bacterial distinguishment and antibacterial applications. ACS Applied Materials & Interfaces, 8(47), 32170–32181. https://doi.org/10.1021/acsami.6b10398
Yavari Maroufi, L., Shahabi, N., Fallah, A. A., Mahmoudi, E., Al-Musawi, M. H., & Ghorbani, M. (2023). Soy protein isolate/kappa-carrageenan/cellulose nanofibrils composite film incorporated with zenian essential oil-loaded MOFs for food packaging. International Journal of Biological Macromolecules, 250, 126176. https://doi.org/10.1016/j.ijbiomac.2023.126176
Yong, Y., Wang, S., Li, L., Li, R., Ahmad, H. N., Munawar, N., & Zhu, J. (2023). A curcumin-crosslinked bilayer film of soy protein isolate and chitosan with enhanced antibacterial property for beef preservation and freshness monitoring. International Journal of Biological Macromolecules, 247, 125778. https://doi.org/10.1016/j.ijbiomac.2023.125778
Yuan, L., Liu, R., Zhou, Y., Zhang, R., Chen, S., Yang, Q., Gu, Y., Han, L., & Yan, B. (2024). Janus biopolymer nanocomposite coating with excellent antibacterial and water/oxygen barrier performance for fruit preservation. Food Hydrocolloids, 149, 109528. https://doi.org/10.1016/j.foodhyd.2023.109528
Zhao, L., Zhang, M., Mujumdar, A. S., & Wang, H. (2022a). Application of carbon dots in food preservation: A critical review for packaging enhancers and food preservatives. Critical Reviews in Food Science and Nutrition, 7, 1–19. https://doi.org/10.1080/10408398.2022.2039896
Zhao, L., Zhang, M., Wang, H., & Devahastin, S. (2022b). Effect of addition of carbon dots to the frying oils on oxidative stabilities and quality changes of fried meatballs during refrigerated storage. Meat Science, 185, 108715. https://doi.org/10.1016/j.meatsci.2021.108715
Zhu, Z., Meng, L., Gao, Z., Liu, R., Guo, X., Wang, H., & Kong, B. (2024). Development of chitosan/polycaprolactone-thymol Janus films with directional transport and antibacterial properties for meat preservation. International Journal of Biological Macromolecules, 268, 131669. https://doi.org/10.1016/j.ijbiomac.2024.131669
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